STUDY THE EFFECT OF ETHANOLIC EXTRACT OF CERATOINA SILIQUA, GLIMEPHAN AND METFORMIN ON SEMEN FLUID QUALITY IN DIABETIC MALE GUINEA PIG INDUCED BY ALLOXAN

This study was carried out in an animal house of the Collage of VeterinaryMedicine/ University of Basrah, An attempt has been done to induce diabetic byalloxan in male guinea pigs and investigation of the effects of diabetic on semen fluidquality. Moreover, the present study aimed to evaluate the ameliorating effect ofethanolic extract of Ceratonia siliqua, metformin and Glimphan in experimentallyinduced in diabetic male guinea pigs. The study was applied on (30) adult male guineapigs, their weight ranged between (800-1000g) and aged between 6-6.5 months. Themale guinea pigs were divided randomly into five groups, each group consist of sixguinea pig as the following:Group1:- Male guinea pigs at (Negative controls) administrated normalsaline3ml orally for 30 days.Group2:- Male guinea pigs at(Positive control) given alloxan (150mg/kg B.W.I.P) for three days and remain for 30 days.Group3:- Male guinea pigs given alloxan (150mg/kg B.W. I.P.) for three days,then treated with Glimphan drug ( 0.1mg/kg orally administration) for 30 days.Group4:- Male guinea pigs given alloxan (150mg/kg I.P.) for three days, thentreated with Metformin drug(15mg/kg orally administration) for 30 days. Group5:- Male guinea pigs given alloxan 150mg/kg I.P. for three days, thentreated with ethanolic extract of Ceratonia siliqua fruit (500mg/kg B.W. orallyadministration) for 30days.At the end of experimental period, the blood samples were collected from heart bycardiac puncture, the serum was isolated to be used for the analysis of biochemicalparameters such as glucose concentration, FSH, LH and testosterone concentrations.The results showed a significant (P≤0.05) increase in glucose concentration of theserum diabetic male guinea pig group as compared with (-ve) control group, whereasthe results were revealed a significant(P≤0.05)decrease of glucose concentrationguinea pigs treated with ethanolic extract of Ceratonia siliqua. The results obtained asignificant decrease (P≤0.05) in body weight, FSH, LH and testosteroneconcentrations in serum and semen, sperm motility, sperm concentration, total spermcell, live-dead sperm and significant (P≤0.05) increase in sperm abnormalities. Whileguinea pigs that are treated with ethanolic extract of Ceratonia siliqua the showed asignificant (P≤0.05) increase in body weight. In addition, this extract improved thereproductive system by significantly increasing FSH, LH and testosteroneconcentrations in serum and semen, sperm motility, sperm concentration, total spermcell, live-dead sperm and significant (P≤0.05) decrease in sperm abnormalities of thediabetic male guinea pigs treated with extract as compared with (-ve) control andanother treated groups.Histological examination showed many pathological changes in pancreas and testisin diabetic group but in those that are treated with ethanolic extract of Ceratoniasiliqua, the histological changes were near the normal status. It is concluded thatgood anti-diabetic activity, hypoglycemia effect and spermatogenic activities. Basedon these results, we suggested the possible utilization of Ceratonia siliqua as atherapy to prevent diabetic and improved the performance of male reproductivesystem as compared with other treated such as glimephan and metformin drugs