MOLECULAR AND SEROLOGICAL IDENTIFICATION OF NEWCASTLE DISEASE VIRUS PROPAGATED IN EMBRYONATED CHICKEN EGGS
2020
Firas T. Mansour Al-Mubarak | Ammal F. Ghanim | Hazim T. Thwiny | Ibraheem K. Younus
The aim of this study is to propagate the non-virulent Newcastle disease virus in thelaboratory, determination the cytopathic effects in the inoculated chicken embryos, andconfirmation of virus growth by serological and molecular techniques by performinghaemagglutination and reverse transcriptase polymerase chain reaction (RT-PCR) tests,respectively. LaSota virus strain which is a live vaccine was used for this purpose. Nine-dayoldembryonated chicken eggs were inoculated with the virus and further incubated for 48hours; and the allantoic fluid was collected for further processing. Petechial haemorrhages andcongestions were observed in the inoculated embryos while in the un-inoculated eggs; theembryos were normal and did not show any lesion. Virus growth in the allantoic fluid wasconfirmed by performing haemagglutination and RT-PCR tests. These results support theisolation of other viruses in our laboratories, which will contribute to perform otherexperiments such as studying virus characteristics and observation of its pathological effects onthe embryos, preparation of viral antigens, sequencing of viral genome, and possiblydiscovering new viruses.
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