Content of fusarium-mycotoxins in some spring barley varieties (artificial and natural infection).

A quantitative immunochemical technique ELISA was used to determine DON content in samples of spring barley varieties inoculated by Fusarium culmorum in 2000 (12 varieties) and 2001 (19 varieties). DON content in the samples from 2000 crop ranged from 5.1 (Forum) to 19.1 ppm (Jersey) with average 9.7 ppm while the range of DON content in 2001 crop was from 1.3 (Madeira) to 8.2 ppm (CI.4196) with average 3.8 ppm. DON content was also determined in another set of 32 samples of spring barley varieties from fields in 24 districts of the CR from 2001 crop and a mycological examination was made using the method of mycologically monitored grains to identify the species of fungal pathogen. ELISA detected DON in all samples of this set; its content ranged from 0.03 to 3.77 ppm, average content was 0.4 ppm, the limit value was exceeded in one sample only. Fusarium graminearum was a prevailing toxicogenic species in spring barley in 2001; its capture coincided with higher levels of DON. It was stated that ELISA and GC were compatible methods for DON determination.A quantitative immunochemical technique ELISA was used to determine DON content in samples of spring barley varieties inoculated by Fusarium culmorum in 2000 (12 varieties) and 2001 (19 varieties). DON content in the samples from 2000 crop ranged from 5.1 (Forum) to 19.1 ppm (Jersey) with average 9.7 ppm while the range of DON content in 2001 crop was from 1.3 (Madeira) to 8.2 ppm (CI.4196) with average 3.8 ppm. DON content was also determined in another set of 32 samples of spring barley varieties from fields in 24 districts of the CR from 2001 crop and a mycological examination was made using the method of mycologically monitored grains to identify the species of fungal pathogen. ELISA detected DON in all samples of this set; its content ranged from 0.03 to 3.77 ppm, average content was 0.4 ppm, the limit value was exceeded in one sample only. Fusarium graminearum was a prevailing toxicogenic species in spring barley in 2001; its capture coincided with higher levels of DON. It was stated that ELISA and GC were compatible methods for DON determination.