Relevance. The studies are aimed at assessing the intravarietal allelic diversity of genes for resistance to fusariosis and cladosporiosis of tomato cultivars bred at the Michurinsky State Agrarian University using molecular markers.Methods. The biological objects of the study are tomato varieties bred at the Michurinsky State Agrarian University. A total of 10 varieties were analyzed. The assessment of intravarietal polymorphism was determined on a sample of 10 plants of each variety. DNA extraction was performed using a Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, USA) for DNA extraction according to the manufacturer's protocol. The P7 DNA marker was used to identify the cladosporosis resistance gene Cf-19. The presence of the fusarium wilt resistance gene I-2 was determined using the marker I-2/5. Amplification results were visualized by electrophoresis in 2% agarose gel.Results. 10 plants of 10 varieties of Michurinsky GAU selection tomato were analyzed using molecular markers of genes for resistance to cladosporiosis and fusariosis. An assessment of intravarietal polymorphism was carried out. In most of the varieties studied, the genes analyzed are identified in a heterozygous state. Three varieties (Vivat, Carotinka, Krasavets) containing only alleles of susceptibility to the causative agent of the disease in the genotype I-2 identified by the gene. Individual samples were homozygous for the fusariosis resistance allele. Analysis of the gene for Cf-19 resistance to cladosporiosis showed that most of the varieties studied are heterozygous forms. In some varieties, all ten plants were heterozygous. These include Krasavets, Vivat, Orlik, Bui Tour. The remaining samples had different allelic compositions. The pathogen resistance allele has been identified in plants of the Nepryadva and Chernysh varieties. The dominant allele in the homozygous state was detected in the Japanese variety. At the same time, all the analyzed plants of this variety were monotonous.Conclusion. It has been established, but a significant part of the varieties of tomato selection of Michurinsky GAU is polymorphic in the genes for resistance to cladosporiosis and fusariosis. In this case, a significant part of the varieties can be used in selection during preliminary molecular analysis. According to the gene for resistance to fusariosis, the following varieties are identified sources of the resistance allele: Sokol, Nepryadva, Yaponchik, Orlik, Zolotnichok, Chernysh. In them, the gene is I-2 represented in a heterozygous state. Intraortic polymorphism was also noted for the Cf-19 gene. Most samples have two alleles. The exception is the Japanese variety, which is the dominant homozygote for this gene.

The morphometric parameters of the internal structure of seeds in seven representatives of the common chervil (Anthriscus cerefolium (L.) Hoffm.) were studied. The variability of the linear dimensions of the seed, endosperm and embryo, the values of the indices IZ/E, IZ/S and IE/S are shown. The analysis of correlation connections between them is given. The studies were carried out at VNIIO, a branch of the FGBNU FSVC. Seeds of the variety Aromatny (standard) had a length of 7.12±0.146 mm. Significantly smaller seeds had two accessions, variety Ogorodnik (6.56±0.158 mm) and No. 21, which is 8.0 and 27.0% less than standard. Seeds of a significantly larger size, including No. 24 and No. 22, respectively, by 3.0-18.0% higher than control. The endosperm length of the standard was 5.20±0.121 mm. The smallest size of the endosperm was noted in sample No. 21, which is 7.1% less than the control. Three samples significantly exceeded the standard (by 7.5-16.5%) in terms of the length of the endosperm, including samples No. 20 and variety Ogorodnik. The length of the embryo in the Aromatny variety is 1.54±0.119 mm. Samples of variety Ogorodnik and No. 21 had an embryo significantly less than the standard by 14.3 and 10.4%. Significantly larger embryos were found in samples No. 24 and No. 22, which is 17.5-44.2% higher than control. The coefficient of variation (V, %) for the parameter seed length varied from 9.17% to 12.3%, for the endosperm length from 8.35% to 14.6%, and for the embryo from 20.1 to 34.8%. The IZ/S index varied from 0.195 to 0.266. The IE/C index varied from 0.707 to 0.930. A close relationship (0.675-0.863) was noted between the length of the seed and the length of the endosperm, with downward deviations to a value of 0.265 in sample No. 21 and Dietichesky variety. The presented information on the example of chervil seeds indicates a significant variability of morphometric parameters. The data obtained can be used for an in-depth characterization of seed quality, substantiation of priming technology.

Introduction. The use of new covering materials is an important modern agrotechnique for cultivating of bell pepper in the open ground, which allows to minimize chemical treatments, increasing the yield, quality and preservation quality of vegetable products.The purpose of this work is to study the effect of various mulching materials (black polyethylene film and spandbond «Agrotex 60 perforated mulch») on the yield of bell pepper and also on biochemical composition of fruits and on their preservation quality in relation to the ripeness phase.Methods. The objects of the study were the fruits of two bell pepper hybrids of domestic and foreign breeding (respectively Nathalie F1 and Anetta F1), grown in the open ground using mulching materials. The experiments were laid according to standard methods adopted in vegetable growing. Storage was carried out in accordance with the methodological guidelines for conducting research on the storage of vegetables.Results. The use of mulching materials contributed to an increase in total yield and the yield of standard products in the crop structure, and also positively affected the accumulation of solids, vitamin C, monosaccharides, disaccharides and as a whole – the sum of sugars. The domestic hybrid Natalie F1 (74.9%), grown using black film as a mulching material, as compared to foreign hybrid Anetta F1 (71.3%), was characterized by better preservation quality. Significant differences in the chemical composition of fruits depending on the ripeness phase were revealed. Fruits laid for storage in full biological ripeness (red) were characterized by an increased amount of weight loss on all storage options. A positive effect on the storage persistence of bell pepper fruits with use of mulching materials on all variants of the experiment was also established.

Relevance. Mash (Vigna radiate (L.) R.Wilczek) is a very ancient Asian legume plant, which, thanks to a number of advantages, may become an important vegetable crop in Russia. Young fruits and seeds of Vigna radiate are rich in protein, vitamins A, B, C, PP, iron, folic acid, and taste like green peas. A lot of essential amino acids and a low glycemic index make it possible to use even mature mash seeds for the preparation of dietary food. To preserve the harvest, it is important to know the diseases of the crop.Methods. For a number of years, a sample of the radiant vigna VR-2014 (Vietnam) has been studied on a natural infectious background in the soil and climatic conditions of the Belgorod region (Russia). Field tests and mycological studies made it possible to determine the species composition of phytopathogenic microscopic fungi, describe the symptoms and compare the main elements of accounting for mycoses.Results. The length of the growing season of the radiant vigna did not exceed 83 days, the appearance of seedlings was noted on 10-12 days from sowing, flowers – on 68-73 days. The height of the plants did not exceed 40 cm. Ascochytosis (the causative agent of Ascochyta boltschauseri) led to losses of 82% of seed productivity, with an average prevalence of 40% and the development of the disease of 2.5 points. Ascochitosis reduced the values of all elements of seed productivity: the length and number of fruits, the mass of 1000 seeds, but most of all (by 5 times) the weight of fruits and seeds from the plant decreased. Peronosporosis (the causative agent of Peronospora fabae) led to losses of seed productivity by 16%, root rot (the causative agent of Bipolaris australensis) and fomosis (the causative agent of Phoma diversispora) – by 17% and 11%, respectively. Spotting was the leading symptom of mycoses of Vigna radiate, and the most harmful phytopathogenic fungi were representatives of the ground-air group of pathogens.

Relevance. Obtaining modern cucumber hybrids is impossible without the constant involvement of genetically new sources in the breeding process. One of the most important tasks in the issue of creating new heterotic cucumber hybrids is to obtain, and in the future, selection for hybrid crossings, parental lines with the most important economically valuable traits. Research is aimed at obtaining new short-fruited cucumber lines with a smooth type of fruit in order to obtain hybrids with their help that combine resistance to powdery mildew for the “pm” and “pmh” genes, tolerance to the zucchini yellow mosaic virus for the “zymv” genes in combined with high yields, the possibility of growing them not only in unheated film greenhouses, but also in glazed greenhouses.Methods. This article presents two schemes for obtaining parental forms using the example of creating four cucumber lines with a short, smooth fruit. Their comparative characteristics and description of new hybrids created with the participation of the studied linear material are carried out. Materials and methods. The main studies were carried out in 2013-2020 on the basis of the Pumpkin Cultures Laboratory of the Krymsk Breeding Center "Gavrish" of the Research Institute of Vegetable Breeding (Krymsk, Krasnodar Territory). The research material is F1 hybrids and selection samples of parthenocarpic cucumber with a smooth type of fruit of various origins (34 samples in total) from the NIISOK collection. The work used traditional methods for assessing selection-significant traits and modern methods of molecular labeling. The purpose of the work is to compare two schemes for obtaining short-fruited cucumber forms, to evaluate the created lines and hybrid combinations with their participation in terms of a complex of economically valuable traits.Results. The result of the work was the creation of new hybrid combinations based on the obtained lines, two of which are included in the State Register of Breeding Achievements. These are F1 Ministar (F1 1600/16) and F1 Promini (F1 1995/16).

Scientific relevance. Plant viruses cause a significant economic loss to potato production, especially if plants are infected at early growth stages and infections are mixed. Viral diseases reduce both yield and quality of harvested crops. Detection and identification of plant viruses are key important to prevent their spreading and to achieve potential yield predetermined by characteristics of varieties.Research methods. Seven potato varieties, bred in Russia and overseas, were used in the field experiment: Smak, Avgustin, Yantar, Laperla, Labella, Red Lady, Sante, Belmonda. Viral infection rate was measured by the percent of plants with symptoms to the total number of plants. In addition to infection frequency, a disease rate was described after visual estimation. Total RNA was isolated from the collected leaves according to Bekesiova I. et al. 1999 [13]. Qualitative and quantitative estimation of plant viruses in the samples were conducted by single-step real-time RT-PCR with fluorescent detection with the Applied Biosystems QuantStudio 5 and commercial kits “Potato Virus X, Y, M, L, S, A, PSTVd-RT” (Syntol Company) according to the official protocol of the kits.Results. As a result of our research, symptoms of mixed viral infection were described for potato varieties depending on concentrations and proportions of these viruses in a plant. Mixed viral infection in the potato field in Primorsky Krai comprised PVY, PVX, PVA, PVS, PVM, also PLRV and PSTVd.

Relevance. Potatoes belong to crops that place high demands on the level of mineral nutrition. He needs nitrogen especially badly. Timely introduction of calculated doses of nitrogen fertilizers based on the results of photometric diagnostics of plants allows rational use of fertilizers and increase potato yields.Material and methods. The studies were carried out in the conditions of the Central region of the Non-Chernozem zone on sod-podzolic medium loamy soils with a high content of mobile forms of phosphorus and potassium and a low humus content (1,9%). The object of research is an early-ripening potato variety Bullfinch. To recalculate the dose of nitrogen fertilizing, photometric diagnostics of crops was carried out with an optical sensor GreenSeeker RT200. Additionally, before and after fertilizing, a manual N-tester Yara was examined. Before the main harvest, a control digging of tubers was carried out to determine the structure of the crop with division into 4 fractions.Results. It was found that the introduction of nitrogen fertilizers into the top dressing during the closing phase of the tops in the form of ammonium nitrate has a generally favorable effect on the growth and development of potatoes. This is confirmed by the results of photometric diagnostics performed by the Yara N-tester during the withering phase of the tops. When applying top dressing with a fixed dose, the N-tester readings were 21,7% higher than in the control, and when applying fertilizers with a differentiated dose by 25,6%. The yield of potato tubers increased when applying a fixed dose of top dressing by 10,8%, differentiated by 14,5% compared to the control.

Vegetable peas are by far the most widely used among the main vegetable legumes. Due to its high nutritional value, it has an important food value and is cultivated almost everywhere. High nutritional qualities of vegetable peas are determined by the content of protein, carbohydrates, dietary fiber, vitamins, as well as macro– and microelements. Pea protein is popular due to its affordable price compared to animal protein. The value of pea protein is determined by its amino acid composition and its high balance, especially valuable amino acids that are not synthesized in animals and humans.The article discusses the nutritional value of vegetable peas (green beans; fresh, frozen and canned vegetable peas; dry seeds); the content of water-soluble protein, amino acid composition, the content of macro- and microelements in the seeds of vegetable peas of FSBSI FSVC selection varieties; the quality of green peas as raw materials for canning, depending on the type of seeds and the structure of starch grains; requirements for the quality of raw vegetable peas for canning; seedlings and microgreens as useful and nutritious products for fresh consumption; medicinal properties of vegetable peas; varieties of vegetable peas for various uses.

Relevance. The genus Allium L. attracts the attention of researchers due to the presence of valuable medicinal, nutritional and decorative properties, adaptive capabilities, resistance to pests and diseases, ecological plasticity in its representatives, which contributes to the competitiveness of species and the manifestation of a high degree of adaptation outside natural areas and wide geographical distribution. Allium resources in Russia are a potential source of genes for expanding the genetic base of agricultural crops.The aim of the work was to establish the yield and change in the quality of green leaves of Allium species introduced into the Moscow region during short-term storage, depending on the type of packaging, storage period and temperature. 4-5-year-old plants of 4 species (A. altyncolicum, A. chyatophorum, A. nutans and A. turkestanicum) were grown on the collection plot of perennial onions of All-Russian Research Institute of Vegetable Growing – branch of the FSBSI FVSC.Results. In the conditions of the Moscow region, the yield per leaf generation varied from 2.7 kg/m2 (A. altyncolicum) to 4.9 kg/m2 (A. cyathophorum). The maximum yield of marketable products was noted in hermetically sealed plastic bags when stored for 10 days at a temperature of +6...8oС in a refrigerator with controlled conditions: A. turkestanicum – 70.0%, A. cyathophorum – 75.5%, A. altyncolicum – 84.9%, A. nutans – 92.9%. However, an increase in the dry matter content (10.1-15.5%) was found during storage in an open polymer box in all tested species. The maximum content of vitamin C during storage in hermetically sealed bags with a density of 100 μm was found in A. altyncolicum (37.1 mg%) and A. nutans (42 mg%). In other species, the maximum value of this indicator was noted before storage. A slight increase in the amount of sugars after storage for 10 days was noted in species with a linear leaf shape when stored in a polymer box.

The priority direction in melon breeding is the creation of new competitively capable varieties that combine resistance to adverse environmental factors with valuable economic characteristics.Materials and methods. The object of the study is melon varieties 599 and 595. The purpose of this study is to study the influence of weather conditions on the yield and biochemical composition of the studied melon varieties in the conditions of the Volgograd Volga region. The research was carried out at the Bykovskaya melon breeding experimental station for three years according to the developed methods, the Osen variety was used as a standard.Results. The article presents the results of the influence of weather conditions on yield, fruit weight, and also presents an analysis of the biochemical composition of melon fruits. A complete description of the studied melon varieties is given. In terms of yield, the high indicators for cultivars 599 and 595 were in 2020 with the totals of average monthly active temperatures of 114°C and precipitation totals of 178 mm, and amounted to 19.0 t/ha and 21.3 t/ha. According to the content of dry matter in the fruit juice, according to the results of studies, it was seen that with the greatest amount of moisture, the indicators were higher in 2018 with a precipitation rate of 296 mm in cultivars 599 – 13.6%, in 595 – 13.2%, than in 2019 with a norm of 272.6 mm 599 – 12.0% in 595 – 11.6%. Thus, we can conclude that these melon varieties meet the modern requirements of the industrial melon industry.