Relevance. From an agrotechnical point of view, beans are a valuable crop capable of greening agriculture. Legumes have the ability to enter into symbiotic relationships with nodule nitrogen-fixing bacteria and assimilate atmospheric nitrogen. The creation of highly efficient plant-microbial systems in agrocenoses by breeding new varieties of legumes with a high symbiotic potential is a promising direction that makes it possible to control the efficiency of plant biomass accumulation and the quality of agricultural products. There is a need to evaluate varieties for adaptability and nodule-forming ability to implement breeding programs to create highly productive and high-quality varieties that can realize the genetic potential of productivity in the sharply continental climate.Materials and methods. On the basis of the Federal State Budgetary Educational Institution of Higher Education Novosibirsk State Agrarian University in 2018-2020, an assessment was made of green bean varieties of different ecological and geographical origin with a bush type of growth. Experimental fields are located in the village of Michurinsky (left bank of Novosibirsk) and the educational and production farm "Garden of Michurintsev" (right bank of Novosibirsk). The study of samples in terms of adaptability parameters, as well as the ability to form nodules, was performed in accordance with generally accepted methods. We took into account the characteristics that affect the productivity of the crop - the number and weight of beans per plant, the weight of one bean, and the yield. The aim of the work is to evaluate the varieties of vegetable beans for adaptability and nodule-forming ability in the conditions of the forest-steppe of the Siberian region.Results. When forming the symbiotic apparatus, the growth conditions were taken into account. It has been established that high rates of biomass are observed on the territory of the village of Michurinsky. Varieties Morena and Maguyra are characterized by intensive growth in the experimental field of the village of Michurinsky, varieties Zoluyshka and Solnyshko – the educational and production farm "Garden of Michurintsev". The total number of nodules per plant varied from 29 (Kormilitsa) to 66. (Zoluyshka), active nodules – from 9 (Kormilitsa) to 49 (Zoluyshka). It is noted that the varieties Solnyshko and Zoluyshka have high rates of general adaptive ability and breeding value of the genotype. A strong relationship has been established between the characteristics of leaf mass and the number of active nodules, the number of active nodules and the leaf surface area; average - between the number of active nodules and plant biomass.

Relevance. In the arsenal of agents with antibacterial activity, a significant place belongs to preparations based on the raw materials of two types of Macleia - Macleaya x kevensis Turill. and Macleaya cordata (Willd.) R.Br. used both in medical and veterinary practice. It is possible to provide the domestic pharmaceutical industry with these medicinal raw materials in full through the development of methods for increasing yields and adapting Maclea to unstable environmental weather conditions.Material and methods. Experiments to study the effect of the use of humates and microfertilizers on these indicators were laid in the fields of the North Caucasus branch of VILAR in 2019-2021. We studied the morphological and economically valuable traits of two species of Maclea, evaluated the growth rates of plants, the yield of raw materials, the content of alkaloids and their collection per hectare under optimal and dry weather conditions.Results. Differences between the two types of Maclea are established in terms of morphological and economically valuable traits. Maclea heart-shaped palmate-lobed leaves are light green in color, the roots are vertical, round-cylindrical, reaching a depth of 50 cm or more, plant height is 300-350 cm. at a depth of 25-30 cm, plant height 200-250 cm. % versus 0.162-0.164% for the heart-shaped Maclea. The dependence of the growth, development and productivity of two types of Maclea on meteorological conditions has been established. Under the conditions of insufficient water supply and high temperatures, the loss of yield in the first year of vegetation in Macleaya cordata is 14%, in the second year - 6%, in Macleaya x kevensis - 21 and 12%, respectively. Foliar treatments with the Normat L complex with Ferovit in the first year of vegetation contributed to a decrease in crop losses and an increase in the yield of alkaloids per 1 hectare in both types of makley, in the second year, with these treatments, an increase in yield by 7-11% was observed, the collection of alkaloids per hectare increased by 14- 18%.

Relevance. The study is aimed at evaluating the biological effectiveness of insecticides to reduce the number of tobacco thrips and gourd aphids in a film greenhouse on gourds. These pests cause significant damage to plants and can carry a viral infection. In greenhouse conditions, tobacco thrips can produce up to 7-8 generations, and melon aphid – up to 16 generations per season. The recommended pest control products have a limited protective duration in protected ground conditions, and in most cases promote the development of resistance in insects.Results. The article presents the results on the use of the insectoacaricide MatrinBio, BP against melon aphids and tobacco thrips, and the biological effectiveness in reducing the number of pests is determined. A single treatment with a bioinsecticide kills up to 90% of tobacco thrips insects. Efficiency against melon aphids is 27% lower. It is noted that the period of the protective action of MatrinBio, BP lasts up to seven days, then decreases. On the fourteenth day, the biological effectiveness against pests is; for thrips – 72%, and for aphids – 40%. For comparison, bioinsecticide Fitoverm, EC, was chosen as a standard, which showed worse results in reducing the number of tobacco thrips (less by 20...25%), but had the best protective effect against melon aphids - by 19...21%. Re-treatment with drugs seven days after the first one significantly reduced the number of pests. To prevent the emergence of resistance in pests in protected ground conditions, it is recommended to include MatrinBio, BP in the integrated protection system and alternate with insectoacaricides from other classes or use in various combinations.

Relevance. The main source of the natural pigment betanin is table beet, known for its medicinal and antioxidant properties, earliness and long shelf life, rich in bioactive compounds, minerals and vitamins. This research was induced by the lack of information required by breeders to increase betanin content in beet. Metabolite profiling is an effective way to assess the interplay between individual metabolites and betanin content in table beet.Materials and methods. The material was selected from the the N.I. Vavilov Institute of plant industry (VIR) collection. Biochemical analysis was based on VIR’s guidelines, and metabolite profiling on gas chromatography, coupled with mass spectrometry.Results. 17 free amino acids were found in the beet root extract. The greatest number of positive correlations with other amino acids (r˃0.72) was found in tyrosine, alanine and phenylalanine. A significant (r = –0.66) negative correlation was observed between betanin and succinic acid, credibly associated with betalamic acid. Sucrose predominated among carbohydrates (95%). Sucrose and maltose showed a weak positive correlation with betanin. Unsaturated oleic and saturated palmitic acids dominated among fatty acids (52% and 20% of total fatty acids, respectively). Earlier-ripening and cold-resistant table beet accessions showed a predominance of unsaturated fatty acids and lower betanin content. The disclosed interactions are important for betanin-rich red beet breeding.

The aim. Today we are witnessing the actualization of research on plant sources for the pharmaceutical and food industries. It is caused by a significant increase in demand for these types of resources. Therefore, a variety of case studies aimed at identifying the characteristics of plant resources, are becoming more actual too. In accordance with this need, the present work aims to identify a relationship between the content of flavonoids in the raw material of such important source of plant raw material as Epilobium angustifolium and soil and climatic conditions and group of raw materials.Methods. At the first stage of this work were applied cartographic, geographical methods and methods of geobotanical description of phytocenoses to determine the most suitable locations for the purposes of the study. At the second stage were applied the method of spectrophotometry to determine the amount of flavonoids in terms of rutin and the method of correlation analysis.Results. As a result of the application of these methods were obtained data on the content of flavonoids in the Epilobium angustifolium raw materials, collected in various soil and climatic conditions, as well as in various morphological groups of raw materials. These data were compared by the method of correlation analysis and after that a positive correlation between the content of flavonoids in the leaves and inflorescences of Epilobium angustifolium was found.Conclusion. It was determined, that the content of flavonoids fluctuation, depending on the Epilobium angustifolium place of growth, was 2.05% in the leaves and 2.5% in the inflorescences of this plant. An average positive correlation of 0.65 was found between the content of flavonoids in the leaf and inflorescences of Epilobium angustifolium. However, the correlation between the content of flavonoids and the latitude of Epilobium angustifolium growth wasn’t identified.

Relevance. Onions in Russia in 2021: Cultivation area – 56.3 thousand hectares; Productivity – 28.6 t/ha; Production – 1608.6 thousand tons; Import from the volume of consumption – 10.7%. Modern varieties and hybrids and plant protection products make it possible to obtain high yields in the conditions of the Non-chernozem zone. The aim of the research is to optimize the parameters and terms of technological operations for the cultivation of onion in an annual crop on alluvial meadow soils of the Non-Chernozem Zone.Materials and methods. The nutrition system of onion plants was tested through distributed nitrogen application during the growing season: Option 1 – (N160P160K160) presowing – (Ca40N10 + K40N20 + K40N20) during the growing season; Option 2 – (N110P110K110) presowing + (Ca40N20 + K40N40 + K40N40) during the growing season. Experience in the use of the microbiological preparation Bisolbi-Plant (Bacillus pumilis) BIS88 together with the 1% humic preparation "Rostok" at a rate of one liter per hectare each to stimulate active growth.Results. At the end of the growing season in the second variant of the nitrogen experiment, the growing season was extended by 7-10 days. The excess yield under the second option for 2021-22 years of research amounted to more than 20.0 t/ha and is significant. The difference in the options for applying the Bisolbi-Plant preparation in 2.8 t/ha with HCP05 – 8.2 t/ha was not significant. The advantage of the variant with the biological preparation is higher plant density by 71.0 thousand units/ha and the absence of signs of bacteriosis. Thus, the distributed application of nitrogen (N110P110K110) presowing + (Ca40N20 + K40N40 + K40N40) during the growing season with irrigation water was significantly more effective than (N160P160K160) presowing + (Ca40N20 + K40N20 + K40N20) during the growing season. There was a tendency to increase the yield and quality of products with the double application of Bisolbi-Plant (BIS88) at a rate of (1.0 + 1.0) l/ha.

Vegetables play a key role in food security and nutrition as the food system shifts from food quantity to dietary quality and health benefits. One of the main reasons for the low production and declining average yields of most vegetable crops is climate change. Important limiting factors in maintaining and increasing vegetable crop yields are rising temperatures, reduced water availability for irrigation, flooding and salinity. Under changing climatic conditions, crop failures, declining quality and increasing pest and disease problems are becoming commonplace and making vegetable production unprofitable. Since many physiological processes and enzyme activity are temperature dependent, they will be greatly affected. Drought and salinity are two important effects of rising temperatures that reduce vegetable crop yields. These impacts of climate change also affect the emergence of pests and diseases, host-pathogen interactions, distribution and ecology of insects, timing of emergence, migration to new locations, and their ability to overwinter, all of which become a major barrier to vegetable production. To mitigate the adverse effects of climate change on the productivity and quality of vegetable crops, sound adaptation strategies need to be developed. Emphasis should be placed on the development of production systems that improve water efficiency and are adapted to hot and dry conditions. Technological practices, such as mulching with crop residues and plastic materials, help maintain soil moisture. Excessive soil moisture due to heavy rains becomes a serious problem that can be solved by growing crops in raised beds. An effective way to solve these problems is to create genotypes that are resistant to high temperatures, moisture, salinity and resistance to climatic conditions, using traditional and non-traditional breeding methods, genomics, biotechnology, etc.

Relevance. One of the most important tasks of agricultural production is to provide the population of the regions with vegetables at the expense of local production. Despite the social significance of the vegetable growing industry, its development in the Central Ciscaucasia at the present stage allows only half to meet the demand of the population. For the Chechen Republic, where the area of arable land is limited, the daikon (Raphanus sativus L. subsp. acanthiformis (Blanch.) Stankev.) Has a certain perspective, especially for cultivation in the summer-autumn period, after early harvesting of vegetables and field crops.Results. Under the conditions of the Chechen Republic, the maximum yield of root crops (29.0 t/ha) and marketability of 65% of the early maturing variety Sasha was obtained with a sowing time of 31.07 and a scheme of 35x10 cm. The highest yield of root crops of varieties Dubinushka, Dragon, Favorit was formed at a sowing time of 17.07 (58.1, 62.3 and 57.3 t/ha, respectively), and variety Moskovsky Bogatyr – at a sowing time of 3.07 (72.3 t/ha) at sowing scheme 70x10 cm.

Relevance and methodology. This article presents the results of a study on the effect of growth regulators and water-soluble fertilizers on laboratory and field germination of pumpkin seeds of various types. The results of observations of the growth and development of seedlings and roots are presented. The research was carried out by laboratory and field experience. It is established that based on the analysis of the data obtained, it can be concluded that growth regulators have a significant effect on the laboratory and field germination of pumpkin seeds of various species. Treatment of pumpkin seeds with growth regulators and water-soluble fertilizers is one of the promising methods of preparation for sowing. Not only field germination depends on high-quality seeds, but also the density of standing plants, as well as growth, development and disease damage. The variety of active substances in the studied growth regulators have a positive effect on the embryo, activating the accelerated process of nutrient absorption, providing increased germination energy and seed growth strength. There are many requirements for seed material. Germination is the main sowing quality of seeds, it is the ability to give seedlings (in the laboratory) or (in the field) shoots for a certain period of time. Germination also depends on the conditions of storage and germination of seeds. Another important property is the germination energy, the seeds should sprout in a certain time. The higher the germination energy, the seeds are considered better.Results. The use of various types of growth regulators in the technology of pumpkin cultivation, as studies show, is one of the promising agrotechnical measures. The best result when soaking pumpkin seeds of large-fruited pumpkin, in laboratory conditions, was achieved in variants with the use of growth regulators Vigor Forte- 98% and Phytozont – 95%. When soaking nutmeg pumpkin seeds, the best option was also shown by Vigor Forte and Phytozont – 95%.

Relevance. Population dynamics (population waves) is an intrinsic quality of population systems. These fluctuations can be induced by various factors, e.g. environmental conditions and resource availability, elements of a food chain from the same or a higher level (competitors, parasites, predators), and diseases. Few researchers have addressed the issue of the population dynamics of Henosepilachna vigintioctomaculata in the Russian Far East. This paper investigates the reasons why the potato ladybird beetle became a pest of introduced cultivated plants.Materials and methods. To collect data on imagines, all research sites were examined at intervals of eight days during the entire period of the study. The examination of the research sites was conducted within one day. All the imagines taken from host plants were marked at the first encounter by puncturing their elytra with a sewing needle (0.4 mm in diameter) according to Hirano. The next generation of beetles was marked with nail polish (in two-three days after emergence) to avoid traumatizing the insects. At the second encounter, these beetles were also marked by puncturing their elytra with a needle. The methods implemented did not have an adverse effect on the survivability of the studied imagines. The beetles were released to the same plants where they had been taken from. The research data were analyzed by Jolly’s method.Results. Overwintered imagines appeared in the fields and began ovipositing at the end of May. The first generation of imagines appeared at the end of June – the begging of July. The second generation of imagines could be observed at the end of July – the beginning of August. A part of the imagines migrated to overwintering sites without ovipositing approximately at the end of August. The overwintered beetles were observed to emerge on the Mongolian oak Q uercus mongolica, the greater celandine Chelidonium majus, and the bird cherry Prunus padus. A typical primary biotope of the potato ladybird beetle was a potato field at the stage of vegetative growth (5-7 leaves) surrounded by the couch grass Elymus repens, the common dandelion Taraxacum officinale, and the perennial sow-thistle Sonchus arvensis. Marking the overwintered and new generations of imagines showed that the presence of the potato ladybird beetle in an ecosystem depended on agricultural and agrometeorological conditions.