Priority directions in tomato breeding for protected ground remain stable productivity ness and quality of fruit, early ripeness, resistant to the most harmful diseases. Creating such varieties is a required component of ecological agriculture. Recently, the demand of appropriate species increases and hybrids of tomato with different colouring of the fruit, which is determined by the contents of xanthophylls and various carotenoids (lycopene, β-carotene, lutein, etc.) with antioxidant properties. According to the state program of research for 2000-2010, the staff of the laboratory of gamete and molecular methods of selection of Federal Scientific Vegetable Center created orange early crop variety Rufina for greenhouses. The article describes the brief history of its creation and characterization on major valuable features. Tomato cultivar Rufina is a source of economically useful traits: early ripeness, resistant to abiotic and biotic stresses, yield, palatability and nutritional value of fruits. Therefore, at present it is used when creating new forms of tomato, adapted to the conditions of various modern technologies protected ground - low-volume cultivation and multi-level narrow column hydroponics (MUG). A perspective starting material was received. These are five productive selection forms for the low-volume technology, haracterized by early ripeness (the beginning of harvesting on the 50-70 day of sowing), the weight of the fruit from 90 to 130 g, resistance to apical rot. For a MUG - two low forms with orange fruits weighing more than 30 grams, created as a result of hybridization with determinants of dwarfish redplant varieties Natasha.

Relevance. Maintaining the phytosanitary condition of vegetable and melon crops is an important component in the technologies of cultivation of new and promising varieties and hybrids, since losses from diseases of various etiologies reach 30% or more. This determines the need for immunological studies of the resistance of vegetable and melon crops to phytopathogens of various etiologies, where the basis of the strategy for creating new varieties and hybrids is the joint work of a breeder and a phytopathologist. To do this, it is important to conduct an annual study of the species composition in order to identify new harmful objects that have a negative impact on the profitability of production, as well as to develop measures to protect vegetable crops from the most harmful and economically significant pests and diseases.Methodology and Results. The extensive experimental network of the Federal Scientific Vegetable Center (FSBSI FSVC) in various ecological and geographical zones allows testing varieties and hybrids of vegetable and melon crops in various soil and climatic conditions, including evaluating them for the most harmful phytopathogens in these regions. This review article briefly highlights the main stages of the history of development and current trends in phytopathological research in Russia and our center. The most significant achievements of scientists of the FSBSI FSVC are presented, aimed at a comprehensive study of the current state of populations of pathogens on vegetable and melon crops, resistance factors controlling the interaction of organisms in pathosystems, including genetic ones, optimization and development of new methodological approaches in breeding for immunity and scientific justification of recommended modern protection systems.

Relevance. New technologies Plenty-type in greenhouse vegetable production is wide spread in some countries of Europe, America, South-East Asia and support with main investors of these countries. Project “Vertical farming” was started in FSBSI “Federal Scientific Vegetable Center” at 2010. The goal of our study is the results of the testing plants Apiaceae family at multy circle hydroponic installation using the natural regulators from glycosides class.Materials and methods. Objects of study: plants Apiaceae family – Coriandrum sativum L. (variety Jubilar), Anethum graveolens L. (variety Rusich), Apium graveolens L. (variety Aeliksir); flavonoid glycoside linarozid from plants Linaria vulgaris Mill. L., steroid glycoside moldstim from seeds Capsicum annuum L.. Methods of study: seed treatment with 0,001% water solutions of glycosides, cultivation of plants Apiaceae family at the multi circle hydroponic construction.Results. First experiment by cultivation of plants Apiaceae family at multi circle hydroponic construction was successful and show, that plants Apiaceae family can cultivate at vertical installations Plenty-type. Reaction on the seed treatment with water solutions of glycosides is depended on the species of plants. Seed treatment with glycosides increased height of plants and weight of leaves of Coriandrum sativum L. Anethum graveolens L. increased germination of seeds after seed treatment with water solution of linarozide. Apium graveolens L. increased germination of seeds, height of plants and weight of leaves after seed treatment with water solutions of glycosides. But water solution of moldstim was more effective in 2020, and water solution of linarozide was more effective in 2021.

Relevance. It was to study the variability of the degree of defeat of promising varieties of table carrots by diseases of various etiologies during storage for several years, to determine the level of their polymorphism based on resistance to various types of pathogens and the effectiveness of selection based on an individual immunological assessment of uterine root crops.Materials and methods. The experimental part of the work was performed in 2017-2020 at the FSBSI "Federal Scientific Vegetable Center" with carrot varietal populations of Margosha and Minor. The identification of the species composition of pathogens, the degree of damage, and the level of resistance of variety specimens were carried out in accordance with appropriate methods and determinants. For the immunological assessment of the resistance of carrot cultivars in vitro, the most virulent isolates of different types of fungi were used from the collection of the Laboratory of Immunity and Plant Protection of the Federal Center for Scientific Research.Results. It was established that in the populations of Margosha and Minor under natural storage conditions, the ratio and degree of spread of economically significant diseases differ significantly in different years. Based on an in vitro immunological assessment, a high polymorphism of the individual resistance of genotypes within both populations to the majority of micromycetes was revealed and the most resistant forms to the most dangerous types of pathogens were identified. Immunological analysis of the resulting offspring confirmed the high efficiency of group selection based on a comprehensive assessment of the stability of individual uterine root crops in vivo and in vitro.

Relevance. Large differences in the climatic conditions by the region Altai by years determine the prevalence and harmfulness of diseases, therefore, monitoring by the dynamics of disease development and assessing their harmfulness are relevant and necessary measures. An important role in modern plant protection technologies against diseases play biofungicides. The main advantages of biofungicides as compared with chemical plant protection products are safety for humans and the environment, short waiting times and the possibility of use throughout the growing season, the absence by risks of the emergence of resistant pathogens, the creation of conditions for the natural self–regulation of agrocenoses.Materials and Methods. The research was carried out in 2018-2020 on the fields of the West Siberian Vegetable Experimental Station of the branch of the West-Siberian vegetable experimental station – branch of the Federal State Budgetary Scientific Institution «Federal Scientific Vegetable Center». In the experiment was used the F1 hybrid Nezhinsky of Siberia. Accounting of the phytosanitary condition of crops were carried out in three periods with the determination by the degree of spread and the degree of development of the disease. Studied were the doses and timing of the use of biological preparations Phytolavin and Phytoplasmin in the fight against cucumber bacteriosis during the growing season. The repeatability of the experience was 4-fold, the placement of plots in 4 tiers, the area of the plot was 10 m2 , the accounting area was 3 m2 . The total area were 0.05 hectares.Results. The applicationof drugs Phytolavin (2 l/ha), Phytoplasmin (3 l/ha), promotes to reduce the pace of development of development of bacterial spotting of cucumber in the open ground. The least development of the disease was noted by using the drugs Phytolavin 2 l/ha, Phytoplasmin 3 l/ha, Phytolavin 2 l/ha, with alternation and an interval of 14 days. By the influence of bactericidal treatments against bacterial spotting of cucumber, a higher weight of seed fruits was obtained in all variants compared to the control. The best result – 11.96% to the control was obtained on the variant by using the preparations Phytolavin 2 l/ha, Phytoplasmin 3 l/ha with alternation.

Relevance. Doubled haploids (DH-plants) are excellent material for genetic research and breeding due to their complete homozygosity. The genus Raphanus from the Brassicaceae family is the toughest to produce doubled haploid plants through isolated microspore culture in vitro (IMC). The study of the causes of disturbed root formation and the development of elements of this stage of technology will significantly increase the effectiveness of the IMC technology for European radish.Methods. The study included three varieties from the collection of the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC): Teplichny Gribovsky, Rozovo-krasniy s belim konchikom and Rhodes. The experiments used a standard protocol for obtaining DH plants using IMC technology in a standard form and with a modification of the rooting stage. The solid MS medium (with agar 7g/L): MS without hormones, MS medium supplemented with IAA at concentrations of 0.5; 1 and 2 mg / L and liquid MSm medium supplemented with 0.1 mg / L kinetin were used for rooting of regenerated plants. All media were supplemented with 20 g/L sucrose. We used three types of techniques for transplanting plant explants onto a solid hormonefree MS medium: planting micro-shoots with their basal part immersed by 2-3 mm into the medium; planting in a well made in a nutrient medium using tweezers under sterile conditions; and landing on the surface of the medium without embedment.Results. In this work, we studied the features of the stage of rooting of regenerated European radish plants in vitro conditions. The transplant technique has been proven to be important for the successful establishment of radish micro-shoots. Plant explants must be planted strictly on the surface of a solid hormone-free nutrient medium MS, without embedment. The use of tubes with bridges made of filter paper and MSm liquid medium with the addition of 0.1 mg/L kinetin for the induction of root formation also showed high efficiency. For plants prone to the formation of root-like structures (RLS) with secondary tumors (ST), multiple dissection of abnormal formations with successive transplants is necessary. Modification at the rooting stage of micro-shoots growing has increased the percentage of successfully adapted DH plants in vivo conditions from 0-14% to 95-98%.

Relevance. Fermentation of cabbage is a common way of processing, which allows to preserve and strengthen its useful qualities. To date, breeders have proposed a significant number of varieties and hybrids of cabbage, each of which has its own individual biochemical characteristics. Traditional sauerkraut is carried out after harvest. Long storage can affect the change in the content of organic acids and sugars in cabbage.Methods. The aim of the work was to study the parameters of the fermentation process of eight varieties and hybrids of cabbage by selection in the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center by the intensity of sugar reduction, accumulation of organic acids, organoleptic parameters and activity of lactic acid microorganisms. Fermentation was carried out after a 5-month period of storage of heads.Results. The results showed that the storage period did not affect the fermentation result of most of the studied varieties and hybrids of cabbage. There are varietal differences in the initial content of mono-and disaccharides, organic acids, which affected the fermentation process. The increased content of malic acid in the variety population Podarok, F1 Severyanka contributed to the slowdown of the first stage of fermentation. Varietal features of the Parus population contributed to the slowdown of the first and second stages of fermentation, which was manifested in a decrease in the rate of destruction of sugars. Variety Zymovka 1474 in the process of processing by fermentation accumulates a significant amount of succinic acid, which can characterize its special difference among the studied varieties and hybrids. The best organoleptic characteristics were distinguished by hybrids of fermented cabbage F1 Likova, F1 Natali, F1 Mechta and F1 Snezhinka. The results of the study suggest that the processing of cabbage by fermentation should take into account its individual varietal biochemical features. This will not only preserve the valuable nutritional properties of the cabbage itself, but also strengthen them by optimizing the natural process of lactic acid fermentation. The result will be the best organoleptic effect achieved by an optimal combination of lactic and citric acids and mono-sugars.

The traditional production of linear material  and hybrids in the selection of red beet is a time-consuming,  long-term process due to the two -  year cycle of plant development, self-and cross-incompatibility,  inbred depression. Significantly  reduce the life cycle of the plant allows the vernalization  at different stages  of  development, including during  the  "steckling". Cultivation  of plants with the use of steckling culture in the selection of red beet has not yet found wide application and has not been studied enough, in this regard, in 2009-2018 on the basis of the Federal  State Budgetary  Scientific Institution “Federal Scientific Vegetable Center”. The material of the study was inbred offspring I1-5, which were studied in several series of independent  experiments using different schemes of growing stecklings and roots (one-year and biennial  cycles).  It is shown that the use of culture of steckling and protected ground for breeding schemes to create lines of red beet is advisable, as it accelerates the  process of  evaluating the  heterogeneity of inbred progenies for earliness, index, root, the manifestation of cytoplasmic  sterility, and also allows you to get a wider range of different  inbred forms due to the high variability  in the offspring. The probability of a valuable fully-sterile ms-forms  higher in the group of early maturing plants with a large mass stecklings and promising self-compatibility  mf-lines - among small fractions of mid and late inbred offspring. The continuous use of steckling culture should be recommended only for the preliminary evaluation  of breeding  material on sign of cytoplasmic sterility, followed by the reproduction of selected promising inbred offspring in the open field ("halves method"),  and at later stages of  the breeding process as a  rapid assessment of crossing combinations on sign of cytoplasmic sterility when checking mf-forms on the fixing ability. The alternation  of the culture of stecklings with the traditional biennial cycle of culture in conjunction with the techniques of recurrent selection and use of different backgrounds  (field, greenhouse),  can improve the performance of the selection and get aligned according to the main economic-important  signs of promising inbred offspring in a shorter period of time.

Relevance. Vegetable beans (Phaseolus vulgaris L.) – a valuable high-protein culture, which has multilateral use in the national economy, is recommended for dietary nutrition. The protein content in green beans and seeds varies from 18 to 30%, which in terms of amino acid composition is at the level of protein in milk and meat.The aim of the research is to create a new indeterminate medium-late resistant to major diseases varieties for farms growing that provide fresh produce (green beans) with a long fruiting period, without fiber and parchment in the leaflets of the green bean.Methods. Studies were carried out from 2008 to 2018 on breeding fields of the Federal Scientific Vegetable Center (Moscow Region, Odintsovo District). Scientific work was started in 2013 with an individual selection from collection sample No. 90K (Republic of Crimea). Since 2015, the constant form was propagated with a two-year test of offspring. All the necessary phenological, biometric and phytopathological observations and counts were performed according to the methods generally accepted for this culture.Results. The article presents data on a new variety of vegetable beans Malume of indeterminate (curly) type of growth. As a result of competitive variety testing in the conditions of the Central Non-chernozem Zone since 2018 to 2019, against a natural infectious background, the new variety significantly exceeded the Fatima standard for the yield of green beans and seeds, was characterized by a high content of mono-, the amount of sugars, starch, and was resistant to major diseases. At the end of 2019 year transferred to the State Variety Test.