Nine Penicillium digitatum isolates were isolated from citrus fruits and tested for their pathogenicity on Navel orange fruit (Citrus sinensis L.). In this regard, the isolates exhibited three different levels of virulence. The isolates were identified morphologically using malt extract agar (MEA) and Czapek yeast extract agar (CYA) for colony characteristics, and MEA for microscopic features. Each one of the isolates has the unique features of P. digitatum, especially the largest conidia (6.1-11.9 µm long X 3.2-8 µm width), and phialides (10.1-21.4 µm long X 4-5.1 µm width). In addition, the isolates were identified on a molecular basis using ITS1 and ITS4 primers to confirm morphological identification. Phylogenetic analysis revealed that there was high variance among the isolates, and there were different relations between some of our isolates and other P. digitatum registered strains originating from different countries all over the world. The sequences were submitted to respective GenBank nucleotide databases with accession No. OR198852, OR198853, OR198854, OR198855, OR198856, OR198857, OR198858, OR198859 and OR198860. We also isolated a new P. digitatum (OB15: OR198859) strain with high virulence and rapid sporulation

Nine Penicillium digitatum isolates were isolated from citrus fruits and tested for their pathogenicity on Navel orange fruit (Citrus sinensis L.). In this regard, the isolates exhibited three different levels of virulence. The isolates were identified morphologically using malt extract agar (MEA) and Czapek yeast extract agar (CYA) for colony characteristics, and MEA for microscopic features. Each one of the isolates has the unique features of P. digitatum, especially the largest conidia (6.1-11.9 µm long X 3.2-8 µm width), and phialides (10.1-21.4 µm long X 4-5.1 µm width). In addition, the isolates were identified on a molecular basis using ITS1 and ITS4 primers to confirm morphological identification. Phylogenetic analysis revealed that there was high variance among the isolates, and there were different relations between some of our isolates and other P. digitatum registered strains originating from different countries all over the world. The sequences were submitted to respective GenBank nucleotide databases with accession No. OR198852, OR198853, OR198854, OR198855, OR198856, OR198857, OR198858, OR198859 and OR198860. We also isolated a new P. digitatum (OB15: OR198859) strain with high virulence and rapid sporulation

The experiment was carried out on three different vegetable crops, Cucumber Cucumis sativus L. (Cucurbitaceae), eggplant Solanum melongena L. (Solanaceae) and okra Abelmoschus esculentus L. (Malvaceae), during summer 2021, to investigate the effect of vegetation type on spider density and diversity. For this study, the pitfall trap method was used to collect spiders from the soil surface to ensure a comprehensive representation of all spider guilds. During this study, 20 species belonging to 6 families were collected. The results showed that a total of 374, 285 and 194 individuals belonging to 18, 17 and 15 species were recorded in okra, cucumber and eggplant fields respectively. Species diversity was greater in okra cultivation. Results also indicated that the Lycosidae family was the most abundant and dominant, representing 71.75%, followed by the Linyphiidae family of 90 individuals representing 10.55% of the whole obtained population. The most dominant species were Wadicosa fidelis 152, 98 and 67 individuals and Pardosa injucunda 111, 69 and 52 individuals in okra, cucumber and eggplant respectively. It is inferred from this study that the type of plant can have an impact on spider communities.

The experiment was carried out on three different vegetable crops, Cucumber Cucumis sativus L. (Cucurbitaceae), eggplant Solanum melongena L. (Solanaceae) and okra Abelmoschus esculentus L. (Malvaceae), during summer 2021, to investigate the effect of vegetation type on spider density and diversity. For this study, the pitfall trap method was used to collect spiders from the soil surface to ensure a comprehensive representation of all spider guilds. During this study, 20 species belonging to 6 families were collected. The results showed that a total of 374, 285 and 194 individuals belonging to 18, 17 and 15 species were recorded in okra, cucumber and eggplant fields respectively. Species diversity was greater in okra cultivation. Results also indicated that the Lycosidae family was the most abundant and dominant, representing 71.75%, followed by the Linyphiidae family of 90 individuals representing 10.55% of the whole obtained population. The most dominant species were Wadicosa fidelis 152, 98 and 67 individuals and Pardosa injucunda 111, 69 and 52 individuals in okra, cucumber and eggplant respectively. It is inferred from this study that the type of plant can have an impact on spider communities.

A study was carried out in a mango orchard on reclaimed land for two seasons in 2019 and 2020. The experiment was implemented on fifteen years old mango trees “Fagri Kalan” budded on seedling mango rootstock and planted at 4 × 4 m. This experiment involved two soil growth stimulants (K humate, magnetite) and foliar application with cobalt. The experiment involved two factors, the first one was soil application with K humate by two levels (50 and 100 g/tree/year) and magnetite by two levels (250 and 500 g/tree /year) plus control, whereas the second factor was a foliar application with Co as cobalt sulfate by three levels (0, 15, and 30 ppm Co). The experiment was carried out in a factorial experiment in split plot design with three replicates whereas each replicate was represented by two trees. Result indicated that soil application with K humate at 100 g/tree followed by magnetite at 250 g/tree or foliar application with Co at 15 ppm alone or the combination between (K humate at 100 g/tree and cobalt at 15 ppm) gave the highest values of yield/tree, fruit weight, TSS, reducing sugars, leaf N, P, K, and Fe content of “Fagri Kalan” mango trees grown under salinity conditions.

A study was carried out in a mango orchard on reclaimed land for two seasons in 2019 and 2020. The experiment was implemented on fifteen years old mango trees “Fagri Kalan” budded on seedling mango rootstock and planted at 4 × 4 m. This experiment involved two soil growth stimulants (K humate, magnetite) and foliar application with cobalt. The experiment involved two factors, the first one was soil application with K humate by two levels (50 and 100 g/tree/year) and magnetite by two levels (250 and 500 g/tree /year) plus control, whereas the second factor was a foliar application with Co as cobalt sulfate by three levels (0, 15, and 30 ppm Co). The experiment was carried out in a factorial experiment in split plot design with three replicates whereas each replicate was represented by two trees. Result indicated that soil application with K humate at 100 g/tree followed by magnetite at 250 g/tree or foliar application with Co at 15 ppm alone or the combination between (K humate at 100 g/tree and cobalt at 15 ppm) gave the highest values of yield/tree, fruit weight, TSS, reducing sugars, leaf N, P, K, and Fe content of “Fagri Kalan” mango trees grown under salinity conditions.

This study examined the macro and trace element contents as well as the cytotoxicity of Laurencia obtusa, Ulva lactuca, and Padina pavonica algae collected from the Mediterranean coast of Egypt (Alexandria coast). The results indicated that the seaweed samples contained high concentrations of Ca, Cl, Mg, K, Na, P and Fe. The Cu and Zn contents of the algae were in the ranges of 0.39-0.95 mg/100 g dw and 0.11-0.49 mg/100 g dw respectively. Fresh red algae contained the highest level of Zn and Cu, whereas the iodine level was 1.52, 1.27 and 1.01 mg/100g in fresh each of Padina pavonica, Laurencia obtuse and Ulva lactuca respectively. Fresh red, fresh green, dry green, dry red, in addition to fresh and dried brown algae had maximum lead (Pb) levels of 0.16, 0.14, 0.13, 0.12, 0.04 and 0.02 g/100 g respectively. Fresh red algae contained the highest Cd and Mn levels, whereas dried brown algae exhibited the lowest Cd and Mn concentrations. Different extracts of fresh and dried brown algae exhibited significant larvicidal activity against brine shrimp. LC50 values indicated that dry samples were more cytotoxic than fresh samples.

This study examined the macro and trace element contents as well as the cytotoxicity of Laurencia obtusa, Ulva lactuca, and Padina pavonica algae collected from the Mediterranean coast of Egypt (Alexandria coast). The results indicated that the seaweed samples contained high concentrations of Ca, Cl, Mg, K, Na, P and Fe. The Cu and Zn contents of the algae were in the ranges of 0.39-0.95 mg/100 g dw and 0.11-0.49 mg/100 g dw respectively. Fresh red algae contained the highest level of Zn and Cu, whereas the iodine level was 1.52, 1.27 and 1.01 mg/100g in fresh each of Padina pavonica, Laurencia obtuse and Ulva lactuca respectively. Fresh red, fresh green, dry green, dry red, in addition to fresh and dried brown algae had maximum lead (Pb) levels of 0.16, 0.14, 0.13, 0.12, 0.04 and 0.02 g/100 g respectively. Fresh red algae contained the highest Cd and Mn levels, whereas dried brown algae exhibited the lowest Cd and Mn concentrations. Different extracts of fresh and dried brown algae exhibited significant larvicidal activity against brine shrimp. LC50 values indicated that dry samples were more cytotoxic than fresh samples.

The study aimed to evaluate the performance of 15 exotic indeterminate genotypes of tomato (Solanum lycopersicum L.) to determine their suitability for cultivation under greenhouse conditions. The study material was obtained from two globally known gene banks, namely, the Center for Genetic Resources of the Netherlands and the U.S. National Plant Germplasm System (GRIN-Global) of the United States Department of Agriculture. One locally registered hybrid, Asya, was used as the control. The experimental layout was a complete randomized block design with three replications. The data collected were vegetative, flowering, and fruit characteristics as well as the number of fruits and both early and total yield per plant. High significant differences were observed among the exotic genotypes and control for all the studied attributes. The mean control values of fruit weight, firmness and pericarp thickness were significantly high, and several of the exotic genotypes exceeded the control values for vegetative, flowering, and yield characteristics under study. The genotypes coded as G.21 (Allround), G.18 (Alicante), G.6 (Marsol), G.7 (Harzer Kind) and G.3 (Robar) are promising for their overall performance in the total yield per plant and can be recommended for further exploitation to produce hybrids.

The study aimed to evaluate the performance of 15 exotic indeterminate genotypes of tomato (Solanum lycopersicum L.) to determine their suitability for cultivation under greenhouse conditions. The study material was obtained from two globally known gene banks, namely, the Center for Genetic Resources of the Netherlands and the U.S. National Plant Germplasm System (GRIN-Global) of the United States Department of Agriculture. One locally registered hybrid, Asya, was used as the control. The experimental layout was a complete randomized block design with three replications. The data collected were vegetative, flowering, and fruit characteristics as well as the number of fruits and both early and total yield per plant. High significant differences were observed among the exotic genotypes and control for all the studied attributes. The mean control values of fruit weight, firmness and pericarp thickness were significantly high, and several of the exotic genotypes exceeded the control values for vegetative, flowering, and yield characteristics under study. The genotypes coded as G.21 (Allround), G.18 (Alicante), G.6 (Marsol), G.7 (Harzer Kind) and G.3 (Robar) are promising for their overall performance in the total yield per plant and can be recommended for further exploitation to produce hybrids.

The experiments were conducted during seasons (summer, au-tumn, winter, and spring) 2020–2021 to study the activities of invertase, adenosine triphosphatase (ATPase), and alkaline phosphatase (AlkP) as well as the determination of total proteins. These activities were determined in the bodies of adult honeybee worker, Apis mellifera, (house and forager bees) after feeding on three different diet groups (A) pollen cake, (B) supplementary diet and (C) control group during the four seasons. Group (A) received 50 g pollen cake + 250 ml honey syrup (2 honey: 1 water)/colony/three-day intervals, group (B) received 50 g Brewer’s yeast chickpea cake fortified with 4.2% pollen + 250 ml sugar syrup (2 sugar: 1 water) while group (C) received 250 ml sugar syrup (1 sugar: 1 water) only. Results indicated that the highest increase in total proteins was found in group B during the autumn season with house bees. Invertase activity was high in group A during the summer season with forager bees. The highest ATPase activity was observed in group B during the spring season with house bees, whereas the highest AlkP activities were found in group A during the autumn season with house bees.

The experiments were conducted during seasons (summer, au-tumn, winter, and spring) 2020–2021 to study the activities of invertase, adenosine triphosphatase (ATPase), and alkaline phosphatase (AlkP) as well as the determination of total proteins. These activities were determined in the bodies of adult honeybee worker, Apis mellifera, (house and forager bees) after feeding on three different diet groups (A) pollen cake, (B) supplementary diet and (C) control group during the four seasons. Group (A) received 50 g pollen cake + 250 ml honey syrup (2 honey: 1 water)/colony/three-day intervals, group (B) received 50 g Brewer’s yeast chickpea cake fortified with 4.2% pollen + 250 ml sugar syrup (2 sugar: 1 water) while group (C) received 250 ml sugar syrup (1 sugar: 1 water) only. Results indicated that the highest increase in total proteins was found in group B during the autumn season with house bees. Invertase activity was high in group A during the summer season with forager bees. The highest ATPase activity was observed in group B during the spring season with house bees, whereas the highest AlkP activities were found in group A during the autumn season with house bees.

The efficiency of three different types of pollen-collection traps namely, Front Drawer Pollen Traps (Type, 1), Bottom Pollen Traps (Type,2) and Plastic Slide Traps (Type,3) were evaluated. The data indicated that in type 2, which had Bottom Pollen Traps, a significantly high amount of bee pollen was collected (383.30g/colony) followed by type 1, (179.37g/colony) while type 3 had the lowest amount of collected bee pollen (123.80 g/colony). The highest amount of bee pollen collected during summer was from type (2) during 20 -29 July, followed by that from the same group during the periods 1-10 July and 9-18 August, and then type (1) during the peri-od 20-29 July. The results also revealed that the lowest amount of bee pollen collected by the traps was during the last week of August and September.

The efficiency of three different types of pollen-collection traps namely, Front Drawer Pollen Traps (Type, 1), Bottom Pollen Traps (Type,2) and Plastic Slide Traps (Type,3) were evaluated. The data indicated that in type 2, which had Bottom Pollen Traps, a significantly high amount of bee pollen was collected (383.30g/colony) followed by type 1, (179.37g/colony) while type 3 had the lowest amount of collected bee pollen (123.80 g/colony). The highest amount of bee pollen collected during summer was from type (2) during 20 -29 July, followed by that from the same group during the periods 1-10 July and 9-18 August, and then type (1) during the peri-od 20-29 July. The results also revealed that the lowest amount of bee pollen collected by the traps was during the last week of August and September.

Four agro-wastes were evaluated as substrates for bioethanol production. Seven of the pioneer isolates in the production of cellulase, xylanase and laccase enzymes were selected for soluble sugar and bioethanol production. The highest level of soluble sugar was produced on sugar beet pulp followed by corn cobs. The experimental design included using soybean okara and sesame husk as nitrogen sources added to the production medium. Extraordinary soluble sugar yields were obtained on sugar beet pulp or corn cobs in combination with soybean okara or sesame husk at the concentration of (0.3%) of either. Different concentrations of corn cobs or sugar beet (5, 10, 15, g/100 m medium) were mixed with optimal concentrations of nitrogen sources. Results showed that 10 g of sugar beet or corn cobs achieved the highest soluble sugar yield. The efficiency of four strains of S. cerevisiae for bioethanol production was investigated, S.cerevisiae (AUMC 14720) recorded the highest level of bioethanol on fermentable fungal broth after four days at 30°C.

Four agro-wastes were evaluated as substrates for bioethanol production. Seven of the pioneer isolates in the production of cellulase, xylanase and laccase enzymes were selected for soluble sugar and bioethanol production. The highest level of soluble sugar was produced on sugar beet pulp followed by corn cobs. The experimental design included using soybean okara and sesame husk as nitrogen sources added to the production medium. Extraordinary soluble sugar yields were obtained on sugar beet pulp or corn cobs in combination with soybean okara or sesame husk at the concentration of (0.3%) of either. Different concentrations of corn cobs or sugar beet (5, 10, 15, g/100 m medium) were mixed with optimal concentrations of nitrogen sources. Results showed that 10 g of sugar beet or corn cobs achieved the highest soluble sugar yield. The efficiency of four strains of S. cerevisiae for bioethanol production was investigated, S.cerevisiae (AUMC 14720) recorded the highest level of bioethanol on fermentable fungal broth after four days at 30°C.

In contrast to the global countries, ectomycorrhizae members are not common in Egypt, however, they are very important in the afforestation of poor lands. Their occurrence in Pinus sp., Clerodendrum sp., and Eucalyptus sp. may help to explain why these trees are the most extensively dominant around the world, especially in Egypt. The identification and characterization of numerous ectomycorrhizal fungi often involve large morphological descriptions of sporocarps alone, which consequently, in some instances, raise arguments about the accuracy of these studies. The present work was achieved to isolate, identify, and characterize three ectomycorrhizal fungi from sporocarps combining morphological and molecular techniques. The morphological characteristics of tested species were assessed and compared to standard taxonomic literature. ITS-rDNA was utilized for molecular analysis using the universal fungal primers ITS1. Identification of these species was confirmed by comparing the sequences of amplified genomes of these species with respective species sequences in GenBank, followed by blast analysis.

In contrast to the global countries, ectomycorrhizae members are not common in Egypt, however, they are very important in the afforestation of poor lands. Their occurrence in Pinus sp., Clerodendrum sp., and Eucalyptus sp. may help to explain why these trees are the most extensively dominant around the world, especially in Egypt. The identification and characterization of numerous ectomycorrhizal fungi often involve large morphological descriptions of sporocarps alone, which consequently, in some instances, raise arguments about the accuracy of these studies. The present work was achieved to isolate, identify, and characterize three ectomycorrhizal fungi from sporocarps combining morphological and molecular techniques. The morphological characteristics of tested species were assessed and compared to standard taxonomic literature. ITS-rDNA was utilized for molecular analysis using the universal fungal primers ITS1. Identification of these species was confirmed by comparing the sequences of amplified genomes of these species with respective species sequences in GenBank, followed by blast analysis.

The combined data from two growing seasons in a field experiment study by using two irrigation systems (surface and subsurface drip) in terms of additional water for sugar beet plants under saline conditions are used to estimate root and sugar yield, water use efficiency and root penetration power at the time of harvest. Water salinity levels of 6000 and 8000 ppm were applied to irrigate sugar beet from the time of planting on October 3 to the time of harvest on April 22 with three additions of water (limited 1750, moderate 2500 and optimum 3250 m3/fed). The results are summarized as follows: The heaviest root and highest sugar yield as well as highest water use efficiency were recorded when using subsurface irrigation system, 6000 ppm water salinity level, and moderate or optimum irrigation water (2500 or 3250 m3/fed). Oppositely, the highest penetration power values were obtained using drip irrigation systems with low water quantity (1750 m3/fed) and high-water salinity level (8000 ppm).

The combined data from two growing seasons in a field experiment study by using two irrigation systems (surface and subsurface drip) in terms of additional water for sugar beet plants under saline conditions are used to estimate root and sugar yield, water use efficiency and root penetration power at the time of harvest. Water salinity levels of 6000 and 8000 ppm were applied to irrigate sugar beet from the time of planting on October 3 to the time of harvest on April 22 with three additions of water (limited 1750, moderate 2500 and optimum 3250 m3/fed). The results are summarized as follows: The heaviest root and highest sugar yield as well as highest water use efficiency were recorded when using subsurface irrigation system, 6000 ppm water salinity level, and moderate or optimum irrigation water (2500 or 3250 m3/fed). Oppositely, the highest penetration power values were obtained using drip irrigation systems with low water quantity (1750 m3/fed) and high-water salinity level (8000 ppm).