The present study was conducted to prepare nano-chitosan particles (CN) by ultrafine grinding  from crude chitosan powder (CC) using a ball mill with 130 numbers of zirconia beads in range from 0.5 to 1.5 mm diameter (75 beads 0.5 mm diameter, 30 beads 1.0 mm diameter and 25 beads 1.5 mm diameter). In addition, physical and rheological properties of chitosan solutions were determined. The results revealed that ultrafine grinding has effectively milled the chitosan particles to nanoscale. Prepared chitosan nanoparticles were characterized by  devices  X-Ray Diffraction (XRD), Zetasizer particle sizes and Zeta potential of chitosan particles (after milling for 30, 60 and 90 minutes). The particle size of nano-chitosan was distributed in a range of 250-600 nm. with a polydispersity index use as an anti-oxidant and antimicrobial with reducing the amount used to deliver the desired purpose. Additional research is required to further investigate the potential value-added utilization of these chitosan derivatives in improving the quality and safety of some food products.

Twenty-one new promising lines in addition to four standard cultivars of snap bean (Phaseolus vulgaris L.) were evaluated under eight environmental conditions (four sowing dates in two seasons of 2015/2016 and 2016/2017). A randomized complete block design (RCBD) with three replicates was used for each sowing date at Barrage Horticulture Research Station (BHRS), Qalubia Governorate, Horticulture Research Institute (HRI), Agricultural Research Center (ARC), Egypt. Data were recorded for some traits i.e., number of days to flowering, pod length, pod diameter, fiber content, total green yield and rust disease severity. The results showed that the linear response of environments was highly significant for all studied characters, indicating the differences between environmental conditions and their considerable influence on these traits. Mean squares of genotypes (G) were highly significant for all studied traits, suggesting the presence of wide range of differences between studied genotypes concerning all investigated traits. Mean squares of the other main sources of variation, i.e., years (Y) and sowing dates (D) were also highly significant for all traits except year for total green yield/feddan trait. The mean square due to environment + (Genotypes × Environment) was significant for all studied traits, indicating that there was a considerable interaction of genotypes with environmental condition in different eight environments. Significance of Genotypes × Environment (linear), reveals that genotypes differed in their considerably across different environments. The linear response of environments was highly significant for total green yield trait, indicating that genotypes differed in their regression on the environmental index. The results of stability analysis conclude that the six new lines G1, G2, G3, G7, G10 and G18, were the most stable genotypes, which gave the maximum total green yield overall the eight studied environments and were adapted to environments for total green yield. It is evident that these genotypes could be used as stress tolerant genotypes under stressed environments.

Facing the food industry in Egypt, the challenges of external and internal negative impact on their competitiveness and access to global markets and the study showed that the number of enterprises operating in this sector amounted to about 37.7 thousand that run around 357 thousand workers. It also represents the food exports of approximately 31.7% and 14.3% of the total value of exports of industrial goods and the College Rankings 2016, the average annual value of the products of that sector, about 21.4 million pounds, accounted for about 31% of the value of industrial production for the same year. The research problem was the small size of the internal and external markets of frozen Egyptian vegetables. Despite the increasing global demand for frozen products, Egyptian exports do not increase at the expected rate. The rate of increase in global demand for frozen food products exceeds 510% annually, Compared to its Egyptian counterparts. The study aimed at studying the internal and external markets of Egyptian frozen vegetables. The study aims at identifying the economic characteristics of the local market for frozen vegetables in general, and in particular for the field sample of vegetable freezing plants and processing them in addition to the internal markets for marketing these vegetables. Prices, categories and consumer attitudes towards them. The study was based mainly  on the available and available data published and unpublished by government agencies such as the Central Administration of Agricultural Economics in the Ministry of Agriculture and Land Reclamation, the General Organization for Industrialization, the Chamber of Food Industries in the Federation of Industries, the Commodity Council for Food Industries, the Holding Company for Food Industries, the Commercial Representation Authority, General for Export and Import Control, as well as foreign trade base data at the National Information Center of the Central Agency for Public Mobilization and Statistics. And the most important results as follows that the difference between the prices of the products, vegetables, frozen for different companies in Super accumulator different varieties where ranked Alabama to buy smile from the above parties between the varieties by about 71% of the total parties the company has a smile, and then are classified Mallow estimated at around 14.8 % of the total group has a company-Bessemer, as expected from the results I classified the grape of the biggest differences between varieties increased by approximately 26.7% of the total team purchase Montana, the following varieties of spinach increased by approximately 24.4 % of the total group company Montana.

Pot experiments were carried out using sand (collected from Ismailia ARS), in the greenhouse to study the effect of biochar application, on K and P release from their natural bearing minerals i.e.,K- feldspar and rock phosphate and\or bentonite on some growth of maize plants parameters, their P and k uptake and the soil available P and K. sixty three pots ever filled with sand(10 kg each biochar was added to all pots expect control at rates 0,1.5 , 3 ton per fed. The studied mineral are K feldspar and rock phosphate as source of K and P respectively. Bentonite was also studied because it is the most common mineral used for reclamation of sandy soil .Each mineral was added at rate of 0,1.5, 3 ton / fed the pots are as follow : biochar alone , biochar + K feldspar and biochar +bentonite and biochar and rock phosphate . maize was planted as the common way in which maize seed 6 grains for each pot inoculated with P and K solubilizing bacteria were planted . the usual N fertilizer (NH4 )2SO4 200kg /fed and irrigated as needed . Maize grow parameter i.e plant height and dry weight of maize plant were measured after 2 months of plantation . K and P as well as their uptake were measure. Results revealed that, the use of biochar at 3 tons fed-1 in combination with 3 tons of any of feldspar, bentonite or rock phosphate enhanced significantly growth parameters of maize plants compared to the control treatments .The highest values plant length and dry matter were were 89.00 cm, 11.4 g pot-1 against 36.6 cm and 2.8 g pot-1 for the control treatments., same treatments increased significantly P and K uptake of maize plants and the soil available p and K compared to the control treatments. The highest significant values in P uptake were 22.5, 18.3 & 16.7 mg plant-1 for the treatments (3 tons fed-1)of biochar with either 3ton rock phosphate ,3ton bentonite or 3 ton K-feldspar .while K uptake were 63.9, 61.8 & 60.1 mg plant -1 for biochar ( 3 tons fed-1 )with bentonite (3 tons fed-1 ), feldspar (3 tons fed-1 ) and bentonite (1.5 tons fed-1 ). Regarding available P the highest obtained values were 49.3 mg Kg -1 for 3 ton biochar + 3ton rock phosphate , 24.1 mg Kg -1 for 3 ton biochar + 1.5 ton bentonite and 24.0 mg/ kg-1 for 3 ton biochar + 1.5 ton K- feldspar. Increase either K- feldspar or bentonite to 3 ton had negative effect on available p .

Bacterial wilt disease caused by Ralstonia solanacearum (Smith) Yabuuchi, et al. (1995) is one of the most important bacterial diseases over the world. This study was planned to control the disease using interaction between biotic and abiotic agents, under greenhouse experiment. In vitro, tested bactericides (gentamycin, ceflaxcin and copper sulphate) were decreased growth of R. solanacerum compared with control treatment. Copper sulphate was the most effective compared with ceflaxcin and gentamycin, but ceflaxcin was moderately effective and gentamycin was less effective against the pathogen. Inhibition zone diameter was increased with increasing concentrations of tested bactericides. Application of different bio-agent isolates (Pseudomonas fluorescence, Bacillus subtitlis, Bacillus megaterium and Serratia mareacescne) were reduced the growth of the pathogen compared with control treatment. B. subtilis isolate was the most effective, but isolates of Ps. fluorescens and B. megaterium were moderately effective and S. marescences isolate was less effective against the pathogen. Greenhouse experiments, disease severity were decreased and potato yield were increased with interaction between bio-agents (Bacillus subtilis, and Pseudomonas fluorescence isolates) and bactericides (gentamycin and ceflaxcin), or bio-agents and resistance inducers factors (salicylic acid and jasmonic acid) or bactericides and resistance inducers factors compared with control treatment. However, interaction between bio-agents as tuber treatment and bactericides as soil drench treatment or interactions between bactericides as soil drench treatment and resistance inducers as foliar treatment were the most effective against the disease and the yield. But, interactions between bio-agents as soil drench treatment and resistance inducers as foliar treatment were moderately effective to control the disease and the yield. But, interaction between ceflaxcin as bactericide and Ps. fluorescens isolate as bio-agent or salicylic acid as resistance inducer were the most effective on disease severity and the yield compared with other treatments, but interaction between B. subtilis isolate as bio-agent and jasmonic acid as resistance inducer were less effective compared with other treatment.

In vitro somaclonal variation via callus is considered as one of the most effective tool for improvement of vegetative propagated crops. Different combinations of cytokinine (BA) and auxine (NAA) were used to induce somaclonal variation from leaf explants of the Egyptian geranium cultivar, Pelargonium graveolens L’Herit, Ait. El Qanater El khayria cv.8. Callus induction and regenerated shoots from callus were evaluated; 2.5 mg/L BA and 0.5 mg/L NAA was the optimal for callus induction and number of regenerated shoots. The obtained plantlets were acclimatized under glasshouse conditions with 80% to 90% survival and were transferred to the open field. The evaluation of growth traits and oil analysis were done for the new 13 clones compared with the mother plant in randomized block design for two successive seasons. Characterization of these new clones for morphological traits and essential oil yield and quality demonstrated the induction of variability in negative and positive directions in some characteristics in comparison with the mother plant. Clone no.7 was the superior clone in herb 2264.7 & 2357.3 g/plant and oil yield 2.436 & 3.065 ml/plant in first and second season, respectively, than the mother plant and other new clones with acceptable content of citronellol 25.63% and geraniol 26.52% as a parameter for volatile oil quality. The genetic variation of the new geranium clones were tested using SRAP technique based on  PCR, the polymorphism was detected using 16  pairs of SRAP primers in combinations as forward and reverse. The genetic differences between new clones and the mother plant were ascertained with SRAP primer pair (me2 em4); a unique profile for clone no.7 was detected in SRAP analysis. The similarity percentage varied between 66.7% to 100% homologies among the new clones and their mother plant. The presence of genetic variation between new clones and the mother plant proved that the calliclones were somaclonal mutants, and the geranium cultivar, El Qanater El khayria cv.8, was improved for high yield in herb and essential oil.

The research aimed at identifying risks facing agriculture from water availability and natural hazards by planning the cropping pattern under low risk, and studying the impact of both climate change and geographical area on the productivity per Feddan. The study estimate risk of cropping pattern in the first scenario was estimated to be about 6.6% under normal restrictions. In the second scenario, it was 3.04% under normal restrictions in addition to stabilize the area of rice at 750,000 feddans, with self-sufficiency of 10% and 20% of wheat and maize The total return on risk under the first and second scenarios was about 73.315 and 75.133 million pounds. The cropping pattern in the second scenario is better than the first, as it achieves a higher net return and also achieves state policies  in terms of reducing the rice area. It also increases the self-sufficiency of  wheat and maize. The effect of climate change and geographical area variation on productivity during the period (2013-2016) showed that their impact ranged from 61 to 92%. There Also was a negative impact of climate change on the productivity per Feddan. except for wheat crop the effect of either wheat has not been determined. The study recommended pulling the optimal cropping pattern, the need to increase the awareness of the seriousness of the negative effects of climate change and the development of awareness programs through the various media, and the production of new varieties afford climate change and salinity, and the use of modern technological methods Such as protected agriculture.

Hydrolytic enzyme producing Trichoderma species have long been recognized as an agent for controlling plant diseases caused by various phytopathogenic fungi. A study was done to characterize four isolates of Trichoderma isolated from the rhizosphere of different crop plants to characterize and identify certain biocontrol related enzymes (β1,3-glucanase and chitinase). Morphological and molecular characterize action of antagonistic ability of Trichoderma species were studied. On the basis of morphological and culture characteristics, the Trichoderma isolates were identified as T. harizanum (1 isolate), T. viride (3 isolates). These isolates were tested against plant pathogens (Rhizoctonia solani, Pythium   and Fusarium oxysporum). The best isolate producing chitinase (overproduction) was T. harizanum from (160 to 0.64) mg/ml. On the other hand the best isolate gave over production β-1; 3-glucanase over production was T. viride from (2250 to 2.20 mg/ml)

The aim of this work was to conduct pot experiment to study the ability of plant roots on solubilizing various sources of phosphorus and factors that may facilitate or inhibit their activity. A split medium – split root technique experiment was conducted to study the effect of CaCO3, pH, organic matter (humic acid) and bentonite on the pH, P solubility in the medium and P concentration in bean plants (Vicia faba var. balady). The changes in pH of the lower solution was recorded, also the root exudate was collected in 500 ml of CaCl2 solution 0.5 × 10-4 M and pH 6.85.  Results showed that the highest recorded total dry weight was found when both N forms were applied in the ratio of 1:4 NO-3: NH+4 or NO-3 alone. The mechanism of solubilizing rock phosphate by exudating protons or organic, amino and other organic compounds is possible at the root surface even in alkaline soil as long as the rock phosphate material was added near the root and organic matter was added to limit the fixing power of the inorganic components as CaCO3 and excess soluble Ca. Also, the availability of P from rock phosphate sources depends on its reactivity value.

The drying industry for vegetable crops, especially the onion crop, is one of the most important food industries that achieve quality and safety of food in addition to open the doors of export. Germany is the main importer of Egyptian dried onions by 28.8% of the average total Egyptian onion exports with an average of 3448 tons as an average for the study period (2013-2017) .Followed by the Netherlands in the list of the most important importers of Egyptian dried onions with an average quantity of about 2816 tons, representing 23.5% of the average Egyptian exports of dried onions. Japan accounted for 9.2% of Egypt's total exports of dried onions with an average of 819 tons, followed by Greece, Belgium, Britain and Brazil respectively. The most important variables effecting on Egyptian dried onion were import price of Egyptian dried onion in the foreign markets and export pric es of the competing countries, as well as the average per capita income of importing countries, as well as their population. It was found that the study of external demand functions for the Egyptian dried onions were low price elasticity of demand (inelastic demand) for Japan. where, the increase in the price of Egypt's dried onion exports to Japan by 1% led to a decrease in the quantity of exports by 0.51% ,While the demand of the United Kingdom  was elasticity equal with unit , where, the increase in the price of Egypt's dried onion exports to the Kingdom by 1% leads to a decrease in the quantity of exports by the same percentage, while it was found that the demand for Egyptian exports of dried onions is flexible for the countries of the Netherlands - Brazil  Belgium, where the price of Egypt exports of dried onions by Ratio of 1% leads to a decline in the amount of exported quantity by 1.36%, 1.7%, 1.8% respectively