is study aimed to evaluate the in-vitro antioxidant activities of Moringa Oleifera Lam (MO) extracts, and their protective effects in acetaminophen (APAP)- induced liver injury in rats caused by oxidative damage. The antioxidants activities of ethanolic, aqueous and hexane extracts of different MO edible parts were investigated by DPPH radical scavenging capacity and malondialdehyde (MDA) assays. The ethanolic extracts of different parts of MO were found to have higher antioxidant capacity compared to the aqueous and hexane extracts. The flowers ethanolic extract has the highest total antioxidants capacity among the other different parts of MO follo wed by leaves, pods, roots and finally seeds. HPLC-MS scanning of ethanolic leave extracts showed the presence of flavonoid derivates Apigenin, quercetin and kaempferol in addition to chlorogenic acid.  In the hepatoprotective study, either leaves or pods extracts (300mg/Kg bw or 600mg/Kg bw stomach tube orally) were administrated to rats one hour prior to administration of a single dose of APAP (4g/Kg bw by stomach tube orally). The hepatoprotective activity of MO leaves and pods extracts were followed for 21 days by observed in the levels of liver markers such as alanine aminotransferase (ALT) and the levels of oxidative damage markers including superoxide dismutase (SOD) and malondialdehyde (MDA) and catalase (CAT), wich analysed and compared between groups pretreated with MO extracts +APAP to those treated with  APAP alone. The outcome of this parameters indicate reduction in the severity of liver damage in group treated with MO extracts + APAP and compared to those treated with APAP alone . Also, histopathological examination of liver tissues of rats treated with MO extracts showed an improvement at the end of experiment.  The results of this study indicate the hepatoprotective properties of MO leaves and pods ethanolic extracts against liver injury and thereby signify its traditional use.

Hepatoprotective effect of ethanol extract of ginger, ginger oil or rice bran oil against fatty liver disease which induced by ethanol stress was investigated in the present study. Thirty six (36) male albino rats were classified into 6 groups as follows: 1- Normal control (NC), 2- Positive control (induced fatty liver by ethanolic stress) (PC+), 3- rats group administered ethanol and ginger extract (GE group), 4- rats group administered ethanol and ginger oil (GO group), 5- rats group administered ethanol and rice bran oil (RBO group) and 6- rats group administered ethanol and DMSO (DMSO  control group, because GE, GO and RBO were dissolved in DMSO as a vehicle). Results revealed that hepatic triglycerides was significantly (p≤0.05) raised to 80.7 mg/g liver, in positive control (PC+), compared to 15.98 mg/g liver in normal control (NC). Also significant increase (p≤0.05) in levels of ALT (69.41 U/L), AST (62.98 U/L) and ALP (121.65 U/L) in PC+, compared to their levels in NC (23.35 U/L), (27.95 U/L) and (73.45 U/L) respectively. In addition, high significant level was observed in serum triglycerides (214.37 mg/dl), total cholesterol (TC) (99.81 mg/dl) and LDL cholesterol (47.75 mg/dl) in PC+, compared with its values in NC group: (74.22 mg/dl), (31.45 mg/dl), (4.21 mg/dl) respectively. However, significant (p≤0.05) decrease was noticed in HDL cholesterol level (9.18 mg/dl) in PC+, compared to NC (12.39 mg/dl). On the other hand, treatment by ethanolic ginger extract (200 mg/kg body weight) showed a hepatoprotective effect which confirmed by remediation the values of hepatic TG, ALT, AST, ALP, TP, Alb, besides serum TG, TC, HDL-C and LDL-C in GE group as compared with their values in NC and PC+. Moreover, treatment by ginger oil (200 mg/kg body weight) and rice bran oil (200 mg/kg body weight) displayed a protective effect in GO or RBO groups, but lower than GE. In addition, ethanol extract of ginger disclosed very high antioxidant activity (IC50 = 18.25 µg/ml) compared to both ginger oil (IC50 = 6714.38 µg/ml) or rice bran oil (IC50 = 1409.57 µg/ml). Finally the present study indicates that ethanol extract of ginger showed hepatoprotective effect more than either ginger oil or rice bran oil.