is study aimed to evaluate the in-vitro antioxidant activities of Moringa Oleifera Lam (MO) extracts, and their protective effects in acetaminophen (APAP)- induced liver injury in rats caused by oxidative damage. The antioxidants activities of ethanolic, aqueous and hexane extracts of different MO edible parts were investigated by DPPH radical scavenging capacity and malondialdehyde (MDA) assays. The ethanolic extracts of different parts of MO were found to have higher antioxidant capacity compared to the aqueous and hexane extracts. The flowers ethanolic extract has the highest total antioxidants capacity among the other different parts of MO follo wed by leaves, pods, roots and finally seeds. HPLC-MS scanning of ethanolic leave extracts showed the presence of flavonoid derivates Apigenin, quercetin and kaempferol in addition to chlorogenic acid.  In the hepatoprotective study, either leaves or pods extracts (300mg/Kg bw or 600mg/Kg bw stomach tube orally) were administrated to rats one hour prior to administration of a single dose of APAP (4g/Kg bw by stomach tube orally). The hepatoprotective activity of MO leaves and pods extracts were followed for 21 days by observed in the levels of liver markers such as alanine aminotransferase (ALT) and the levels of oxidative damage markers including superoxide dismutase (SOD) and malondialdehyde (MDA) and catalase (CAT), wich analysed and compared between groups pretreated with MO extracts +APAP to those treated with  APAP alone. The outcome of this parameters indicate reduction in the severity of liver damage in group treated with MO extracts + APAP and compared to those treated with APAP alone . Also, histopathological examination of liver tissues of rats treated with MO extracts showed an improvement at the end of experiment.  The results of this study indicate the hepatoprotective properties of MO leaves and pods ethanolic extracts against liver injury and thereby signify its traditional use.

Hepatoprotective effect of ethanol extract of ginger, ginger oil or rice bran oil against fatty liver disease which induced by ethanol stress was investigated in the present study. Thirty six (36) male albino rats were classified into 6 groups as follows: 1- Normal control (NC), 2- Positive control (induced fatty liver by ethanolic stress) (PC+), 3- rats group administered ethanol and ginger extract (GE group), 4- rats group administered ethanol and ginger oil (GO group), 5- rats group administered ethanol and rice bran oil (RBO group) and 6- rats group administered ethanol and DMSO (DMSO  control group, because GE, GO and RBO were dissolved in DMSO as a vehicle). Results revealed that hepatic triglycerides was significantly (p≤0.05) raised to 80.7 mg/g liver, in positive control (PC+), compared to 15.98 mg/g liver in normal control (NC). Also significant increase (p≤0.05) in levels of ALT (69.41 U/L), AST (62.98 U/L) and ALP (121.65 U/L) in PC+, compared to their levels in NC (23.35 U/L), (27.95 U/L) and (73.45 U/L) respectively. In addition, high significant level was observed in serum triglycerides (214.37 mg/dl), total cholesterol (TC) (99.81 mg/dl) and LDL cholesterol (47.75 mg/dl) in PC+, compared with its values in NC group: (74.22 mg/dl), (31.45 mg/dl), (4.21 mg/dl) respectively. However, significant (p≤0.05) decrease was noticed in HDL cholesterol level (9.18 mg/dl) in PC+, compared to NC (12.39 mg/dl). On the other hand, treatment by ethanolic ginger extract (200 mg/kg body weight) showed a hepatoprotective effect which confirmed by remediation the values of hepatic TG, ALT, AST, ALP, TP, Alb, besides serum TG, TC, HDL-C and LDL-C in GE group as compared with their values in NC and PC+. Moreover, treatment by ginger oil (200 mg/kg body weight) and rice bran oil (200 mg/kg body weight) displayed a protective effect in GO or RBO groups, but lower than GE. In addition, ethanol extract of ginger disclosed very high antioxidant activity (IC50 = 18.25 µg/ml) compared to both ginger oil (IC50 = 6714.38 µg/ml) or rice bran oil (IC50 = 1409.57 µg/ml). Finally the present study indicates that ethanol extract of ginger showed hepatoprotective effect more than either ginger oil or rice bran oil.

A total of 12, 36-week-old of Muscovy drakes were divided randomly into three equal groups (4 individuals each) and subjected to supplementation of VE-Se in combine form in drinking water. The first group was served as control group (C), while the second and third groups (T1 and T2) were supplemented with 1.0g/l and 1.5g/l of VE-Se in drinking water, respectively. The study was performed at poultry research station which belongs to department of poultry production, faculty of agricultural, Ain Shams University and lasted 30-dayes period during summer season. Semen quality traits in terms of ejaculate volume (ml), spermatozoa concentration (×109), sperm motility (%), dead and live (%), PH value and abnormal sperms (%) were determined. Sperm reserves in ducts, seminiferous tubules diameter (STD) and relative spermatogenic activity were also studied. The obtained results indicated that addition of vitamin E-Se improved ejaculate volume, sperm concentration and motility score, while reduced the percentage of abnormalities and dead sperms. Results showed that semen ejaculate volume was significantly increased (P<0.05) with increasing the level of VE-Se supplementation in drinking water. Spermatozoa concentration, progressive motility (%), and packed sperm volume were increased significantly with VE-Se treatment. The percentages of dead and abnormal spermatozoa were significantly decreased (P<0.01) in VE-Se treated compared with the control drakes. Concerning with the effect of two levels (T1 and T2) of VE-Se supplementation in drinking water of Muscovy drakes on the morphometric traits of testis, the obtained results showed that highly significant effects of antioxidant (VE-Se) supplementation on the morphometric of seminiferous tubules (p < 0.05) and the higher values of sperm reserves and spermatogenic activity percentage in both T1 (4.4/ml×109 and 82.8%) and T2 (4.5/ml×109 and 83.0%) compared with control group (3.2/ml×109 and 61.3%). We conclude that VE-Se supplementation could lead to greater economic benefits through increased reproductive efficiency within the ducks production industry.

This study was carried out through two consecutive seasons 2016 and 2017 to investigate the effect of spraying with some antioxidants on vegetative growth, yield, fruit quality and nutritional status of twelve years old Navel orange trees on sour orange rootstock grown in a private orange orchard in Qalubia Governorate, Egypt cultivated in a silty clay loam soil. This experiment involved three antioxidant materials named (salicylic acid, citric acid and folic acid) and each one spraying by three levels of each of with (250, 500 and 1000 ppm) from each salicylic, citric and folic acid plus control treatment so the experiment included ten treatments. Selected trees were spraying three times (at the beginning of the growth cycle in March, just after fruit setting in May and just after June drop in July) by aqueous solution of salicylic acid, citric acid and folic acid until the point of runoff. The highest values of vegetative growth were obtained by spraying 500 ppm salicylic acid, 1000 ppm citric acid and 500 ppm folic acid. Regarding to yield, results pointed out that the foliar spraying with 500 ppm salicylic acid, 500 ppm citric acid and 250 ppm folic acid gave the highest values of yield. Treatments 500 ppm salicylic acid, 250 ppm citric acid and 250 ppm folic acid gave the highest values of physical and chemical fruit properties. In respect to nutrients content, the highest values of macronutrients were obtained by spraying 250 ppm folic acid, while that spraying 1000 ppm salicylic acid and 250 ppm folic acid gave the highest values of micronutrients. Therefore, using treatments 500 ppm salicylic acid, 500 ppm citric acid and 250 ppm folic acid may be recommended from economical point of view and as good treatments for enhancing Navel orange growth, yield, fruit quality and nutritional status especially under old citrus orchards.