Studies were accomplished to identify the important factors affecting successful virgin queens production in Sohag during 2007 and 2008 years. In order to find out the best teaching for rearing virgin queens by grafting method. Markedly differences in the mean number of virgin queens between single and double grafting methods, specially in spring and autumn seasons; in spring mean number of accepted cells of 43.77 with 37.66 emerged queens and 49.85 with 47.07 emerged queens were obtained when single and double grafting were used methods respectively. Spring season was favorable for queens rearing, followed by autumn and summer, while winter season came the latest. There was a clear relationship between larval age at grafting and the larval acceptance, one day old worker larvae gave the highest number of virgin queens emerged. On the other hand, the queen must be removed outside the hive 48 hrs before starting larval grafting to obtain the highest numbers of accepted queen cells. In general, double grafting, situating the cell cups frame in the hive center of the hive and fitting the cell cups on the top and bottom bar, gave the best method for queens acceptance and queens production. However, cell cups fitted on the edges of the bars were successfully reared when ambient temperatures were high in spring season, but queen cells in the center of the hive's brood nest were correlated with higher temperature and had a greater chance for emergence.

The effect of both beekeeping processes (compressed bees Cb and traditional beekeeping Tb) and food diets (vitamins mixed with pollen grains, pollen grains only and plain sugar syrup) on the colony build up were studied during February – April, 2004, at the apiary belonging to Faculty of Agriculture, Ain Shams University. Feeding colonies with supplementary vitamins plus pollen grains resulted after four brood cycles significantly high daily rates of rearing brood (523 and 434 brood cells for Cb and Tb, respectively) and drawing combs (4.31 and 1.61 combs for Cb and Tb, respectively) compared to either unique pollen grains (432 and 338 brood cells and 3.2 and 1.19 combs for Cb and Tb, respectively) or plain sugar syrup (313 and 219 brood cells and 2.1 and 0.79 combs for Cb and Tb, respectively). On the other hand, the worker’s longevity recorded vice versa which were 21.2  26.2, 23.8 days for colonies fed on vitamins plus pollen grains, pollen grains and sugar syrup, respectively. The colonies which fed on vitamins plus pollen grains, pollen grains and plain sugar syrup, their workers were hoarded 236, 220 and 191 mg of sugar syrup / 3 days, respectively.

Experiments were carried out on honey bee colonies (Apis mellifera) during the winter of 2008 and spring of 2009 using colonies wintered with five levels of varroa infestation (0.0, 3.8, 9.0, 13.1 and 22.9%) in the Central Region of the kingdom of Saudi Arabia. The goal of the present work is to determine varroa treatment thresholds and colony collapse level. Response variables examined included varroa mite infestation level (%), cm2 of brood number of frames of brood, number of frames covered with adult honey bees and mites recovered on sticky boards. The data obtained reveled that no significant differences were noticed in cm2 of brood, number of frames of brood, and number of frames of bees in colonies (range of 0.0 to 9.0% varroa infestation or 0.0 – 0.9 mites/bee). Colonies with 13.1% infestation (0.13 mites/bee) exhibited a significant reduction in all measures of performance. The present results suggested that honey bee colonies can tolerate a level of 9.0% varroa infestation (0.9 mites/bee) during the winter without evidence of colony injury. It also suggested that 13.1% infestation (0.13 mites/bee) is the colony collapse or economic injury threshold in the Central Region of the Kingdom of Saudi Arabia. Also colonies with 12.3 or more mites falling on sticky boards after 48-hour period were likely to have lower populations of bees and brood the following spring.

Laying workers expressed as Workers with Activate Ovaries (WAO) having ma-ture oocytes had been studied in colonies headed with natural mated carnica queens. The results showed that the WAO were affected by the age of the queen, where it reached 0.8, 1.3 and 2.2 % for colonies headed with queens 10, 20 and 30 month old, respectively. The worker ovaries started to activate when the reached 22. 18 and 12 days from emergence for colonies headed with caged mated queens, caged virgin queens and queenless colonies, respectively. Also, when the worker's age reached 30 days, the percentages of WAO occurrence reached 3.3, 7.3 and 14.7 % for the corre-sponding colonies status, respectively. In preparing swarm boxes, when the bee-keepers failed to introduce the queens into the nuclei until two days, one week and two weeks, the WAO recorded 1.4, 4.8 and 9.2 %, respectively. In case of queen rearing, the presence of WAO was not significant until the fifth day, and then in-creased gradually to reach an average of 4.6% in the tenth day.