Forty-eight lactating Holstein Friesian cows were utilized to assess impact of supplementing probiotic ZAD (mixture of live bacterial cells and enzymes) compared with T5X (antitoxins product) on some productive, reproductive and antitoxins activity parameters. we have divided the animals to three experimental categories (16 each). The first category, control treatment, was fed basal diet without any supplements. The second category, ZAD treatment, was fed basal diet supplemented with ZAD probiotic (1.1 L/ton). The third category, T5X treatment, was fed basal diet supplemented with T5X antitoxins product (1.1 Kg/ton). Milk yield was recorded. we have collected and analyzed milk samples, blood samples and feed samples in order to constitutes. Time-interval between calving and fertilizing artificial insemination and pregnancy rate were recorded. The gained outcomes demonstrated that milk yield was significantly raised by ZAD treatment than other treatment. Milk components yield were significantly raised by ZAD probiotic and T5X treatments than control. Blood serum total protein, albumin, globulin, glucose, urea and total lipids were not significantly influenced by treatments. Total aflatoxins in concentrate mixture and aflatoxin M1 in milk were significantly diminished by ZAD and T5X than control. Number of days between calving and fertilized artificial insemination were diminished by treatments than control. Pregnancy rate was significantly raised by ZAD  probiotic than T5X and control treatments, respectively. The overall conclusion of this study illustrated that Probiotic ZAD, potentially, has antitoxins activity leading to reinforcing the productive and reproductive performance of lactating cows.

A study was conducted to investigate the effects of feeding different levels of threonine and tryptophan on growth performance, some serum parameters and carcass traits of broiler chicks under Egyptian summer conditions. Three hundred and sixty Ross 308, one-day-old broiler chicks were randomly allocated into nine treatments with 4 replicates of 10 chicks each. Three levels of threonine (100, 150 or 200 %) and three levels of tryptophan (100, 150 or 200%) according to dietary requirements for Ross 308 chick's guidebook were used in a factorial design arrangement to obtain nine experimental treatments. Two periodical diets were used (starter phase 1-21 days and finisher phase 22-35 days). Growth performance traits including live body weight (LBW), Body weight gain (BWG), feed consumption (FC) and feed conversion ratio (FCR) were recorded at the end of each week. Carcasses were manually eviscerated and weighed. Components of total protein, albumin, cholesterol, and triglycerides were analyzed by using commercial kits. The results showed that chicks fed diet containing 200 % threonine had an improvement in live body weight, body weight gain and feed conversion ratio. The performance index increased with the addition of threonine and tryptophan in the broiler diets. Serum total protein and globulin concentrations were affected by supplemental threonine and tryptophan. Dietary threonine, tryptophan did not affect serum albumin and triglycerides. Total cholesterol was increased with supplementation of threonine, tryptophan and interaction between them. Chicks fed 100% dietary threonine had the significantly better results in terms of the relative weights of carcass, edible parts and bursa compared to the chicks fed other dietary threonine levels, although tryptophan amino acid had not any significant effect on the above-mentioned traits. Giblets, liver, and heart weights percentage increase with increasing tryptophan level in the broiler diets. The relative weight of gizzard reduced with increasing level of both amino acids in the diets.In this study, it concluded that supplementation of threonine and tryptophan improves productive performance and health status of broiler chicks without imposing any toxicity as all blood components were in normal range.