A pot experiment was carried out to evaluate the effect of arbuscular mycorrhizal fungi (AMF) inoculation timing on growth and flowering of Chrysanthemum morifolium cuttings. AMF inocula were either directly applied to cutting (AMFC), or applied at transplanting stage (AMFT). The data showed: a significant difference in plant growth of AMF treatment compared with non-inoculated treatment at transplanting stage. Rooting rate in AMF treatment was 99% whereas it was 77% in non-mycorrhizal inoculated. The colonization rate was 53.9% in AMF treatment, while no in non-AMF treatment. Tap root length and number of lateral roots in AMF treatments were twice of those recorded for non-AMF treatments. Inoculation of AMF significantly increased shoot and root growth at transplanting stage. After transplantation, chrysanthemum plants in AMFC and AMFT treatments had 76.42 and 64.24% colonization rate, respectively. Plant height, leaf area, root length, fresh and dry weight of shoots, stems and roots in AMF inoculation treatments (AMFC and AMFT) increased significantly than those of control plants. AMF inoculation significantly shortened flowering time compared with non-AMF plants. Fresh weight, width and length of flowers in AMFC and AMFT treatments were generally higher than those in control. However, a significant increase in fresh weight, width and length of flowers was found in AMFC compared with AMFT treatment. A significant increase of macronutrient concentrations in leaves was observed for AMFC treatment compared with control. Mn concentration in AMFC and AMFT was more than double of that in control. In roots, macro and micronutrient concentrations were generally higher in AMFC than AMFT or control treatments