he present experiment was conducted to determine the optimal source and level of phosphorus supplementation in commercial feeds with the highest growth rate and the lowest releasing in environmental and protect water quality of Nile Juveniles tilapia (Oreochromis niloticus). Fish were divided into twelve groups (groups) each group was stocked into two aquaria each contains 10 fish.  Fish were fed diet contained different levels of phosphorus (0.005, 0.007, 0.009 and 0.011%Kg diet phosphorus) from   three different sources of phosphorus (mono calcium phosphate, mono potassium phosphate and mono sodium phosphate) for a period of 12 weeks. Results showed that average daily gain, specific growth rate, protein efficiency ratio and feed conversation ratio were significantly improved at (p) 1.1% dietary phosphorus level with mono sodium phosphate as the source of phosphorus, mono calcium phosphate represented the highest significant (P<0.05) value of growth and blood parameters (Packed cell volume (PCV), Hemoglobein(Hb) ,red blood cell(RBC) and white blood cell(WBC) in Oreochromis niloticus .  Concerning levels of phosphorus the result clearly indicated that the 0.9% phosphorus level recorded the significant (P<0.05) highest growth and blood parameters in Oreochromis niloticus. Body composition analysis showed that the whole body protein and ash content were increased linearly but lipid was decreased (P<0.05) with increasing dietary of phosphorus.