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EFFICIENCY OF CERTAIN INSECTICIDES ON WHITEFLY LEAF CURL VIRUS AND THEIR RESIDUES IN TOMATO FRUITS
2005
Ibrahim Soad, A. | Elias Nadra, M. | M.Y Ola, | El-Adl Fathy E. | El-Sheemy M.KH.
The work was conducted to clarify the efficiency of certain insecticides on the population of whitefly B. tabaci and incidence of yellow leaf curl virus (TYLCV) in tomato field. The experiment was carried out during Nili plantation of two successive seasons at El-Fayoum governorate. The obtained data showed that the alternate use of etofenprox /thiamethoxam; imidacloprid/ thiamethoxam; acetamiprid/ etofenprox; single continuous application of etofenprox; etofenprox / imidacloprid; acetamiprid/ imidacloprid; thiamethoxam/ acetamiprid; imidacloprid; thiamethoxam and acetamiprid gave excellent initial mortality over 90% on the adult stage of B. tabaci and incidence of tomato yellow leaf curl virus without significant differences between treatments. Thimethoxam as soil drench proved to be the most effective insecticide against adult and immature stages of whitefly, while acetamiprid achieved the lowest mortality for these two stages as well as short residual mortality. On the other hand, alternation of etofenprox / imidacloprid showed the highest initial mortality on nymphs. Residue levels in tomato fruits were also investigated at 30, 45 and 60 days after application of tested insecticides. For imidacloprid residues were found in amounts nearly above maximum residue levels MRLs 30 days while degraded to amounts below MRL by the progression of time to 45 and 60 days after application. Application of imidacloprid in alternate spray program with thiamethoxam; etofenprox; acetamiprid resulted in residues below MRL at the 3 preharvest intervals. Acetamiprid when used at the recommended rate showed the residue of 0.36 mg/kg at early season and then decreased to 0.08 mg/kg at late season. Alternate use of acetamiprid with thiamethoxam; etofenprox or imidacloprid reduce MRLs in tomato fruits. The successive applications of etofenprox alone at the recommended rate resulted in residues above MRL after 30 and 45 days and approximately near MRL at 60 days. On the other hand, the half rate in alternative use with imidacloprid; acetamiprid or thiamethoxam showed residues below MRL at all sampling intervals. Thiamethoxam when used in single successive applications gave residues of 2.5, 1.9 and 1.5 mg/kg at the 3 preharvest intervals, respectively
Mostrar más [+] Menos [-]EVALUATION OF PHYTOCHEMICAL COMPOUNDS IN Moringa oliefera Lam. EXTRACTS ON CYTOTOXICITY OF MCF-7 CELL LINE
2019
Marwa Abd El Alem | S. Ali | M. Sarwat | S. Hussein
Moringa olifera Lam. which called "Miracle tree" was an enormous usage of medical, nutritional and economic benefits so its leaves and roots extracts were vitro examined for phytochemicals contents, antioxidant, and cytotoxicity against MCF-7 cell line. Leaves and roots both extracts were estimate using 2,2-di phenyl-1-picryl hydrazyl (DPPH) radical scavenging assay and high performance liquid chromatography (HPLC) to determine phenolic and flavonoids compounds. The leaves aqueous and ethyl acetate extracts contained a significantly (p<0.05) more phenolic compounds than root extracts, while roots ethyl acetate extracts were significantly more active in DPPH assay comparing with aqueous extracts . However, the leaves aqueous extract and roots ethyl extracts were contained high values flavonoids compound but in case of phenolic compounds the aqueous roots ex tract recorded the greatest highest number followed by the leaves ethyl acetate extract. The ethyl acetate extractions for leaves and roots have a high inhibition percentage against breast adenocarcinoma MCF-7 cell line comparing with aqueous extracts.
Mostrar más [+] Menos [-]STUDY OF CONTAMINATION LEVEL OF OCHRATOXIN (A) IN IMPORED GRAINS WHEAT AND ITS PRODUCTS AT SOME LIBYAN FACTORIES
2016
Altaher Alzwei | Najah Elfeturi
This study was conducted to determine the present of Ochratoxin (A) (OTA) to the number of 50 samples of wheat grain from varieties of soft wheat (Tricticm astivum) and hard (T. durm), and its products from some Libyan Mills (from the north-west and south-east and north) by using a technique (IAC) immunoaffinity columns cleaning and HPLC-FD to detect, with an overall average rate of recovery of the samples of wheat and grain products and fungal isolates 86.59 ± 0.03% and 89.19 ± 0.01%, respectively. The results showed that 96% of the samples were positive for OTA, with the average concentration of OTA from different regions reached the highest concentration of 0.0928μg/kg for soft wheat. The average concentration of the OTA in the different areas that have been grain assembly resulted the highest concentration of 0.0918 μg kg-1 for soft wheat for the Western Region, followed by 0.0783 and 0.020 < μg kg-1 for the eastern and southern region respectively, while the average concentration of the OTA for hard wheat 0.0307 and 0.3316 μg kg-1 of eastern and western region respectively. However all the samples contain levels of OTA are below the limit recommended by Libyan Standards Specification and European Commission Regulation Standard. Results of statistical analysis also showed no significant differences of (P≤ 0.01) of the concentration of the for hard and soft types wheat between regions , and for soft wheat products between regions while there was significant differences between hard wheat products at level of (P≤ 0.01). The results showed that the overall average for the moisture content of the samples by region ranged between 10.6, 11.10% for soft wheat for the southern region and western region respectively, 9.54 and 10.08% for hard wheat to the western and eastern region respectively. Results also showed that the overall average for the moisture content of soft wheat products (flour) between different regions was (13.03, 11.78, 8.58%) to the north-east, north-west and south-west respectively. Hard wheat products did not exceed 13.33, 10.76% for the southern- west and north-east reigions of semolina and pasta respectively, while was within the limit to the Codex Alimentarius Commission. the results of the statistical analysis and the presence of highly significant for moisture content between types of soft wheat and hard within regions and this also applies to soft wheat and hard products between the regions at the level of (P≤ 0.01).
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