Two hundred and ten unsexed broiler chicks (Arbor Acres) at 7 day of age were used to study the effect of dietary supplementation of different Copper (Cu) forms i.e. inorganic (copper sulfate, CuSO4), organic (copper methionine Cu2-Met) or nano inorganic copper particles (Cu-NP) on the productive performance, hematological and biochemical constituents of blood and immune response of broiler chicks. They were randomly divided into seven dietary treatments with five replicate cages per treatment, six chicks for each. The first group was fed the basal diet without any supplementation (control); while the 2nd and 3rd groups were fed the same diet supplemented with 50 and 100 ppm of inorganic Cu (copper sulfate, CuSO4), respectively. The 4th and 5th groups were fed the basal diet supplemented with 50 and 100 ppm of organic Cu (copper methionine Cu2-Met), respectively. The 6th and 7th groups were fed the basal diet supplemented with 50 and 100 ppb of nano inorganic copper (Cu-NP), respectively. Results showed that chicks fed the basal diet supplemented with different forms of Cu had significantly better LBW, BWG, FCR, economic efficiency and production index. Both organic and nano Cu-fed groups showed significantly better productive performance traits compared with the inorganic Cu – fed groups. Supplementation of different Cu forms decreased serum levels of total lipids, triglycerides, cholesterol, low density lipoprotein (LDL), whereas RBCs count, hemoglobin, PCV, glucose, globulin, thyroid hormones (T3-T4), immunoglobulin (IgM, IgG), and antioxidant enzymes activity (TAC– GPX- GSH-SOD) were significantly increased compared with the control treatment. Additionally, Cu supplementation increased lactobacillus sp. while decreased total bacterial count (Salmonella, E. coli and Proteus) compared with the control group. In conclusion, Cu supplementation improved the growth performance, immune response and physiological status of broiler chickens. Broilers fed organic and nano Cu-supplemented diets had better growth performance and immune response than those fed inorganic copper.