Two field experiments were conducted during the summer season of 2003 and 2004 to study the effect of organic and bio-organic fertilizers [esterna biofert, compost, biocompost and effective microorganisms (Em)] on vegetative growth, yield and physical and chemical components in tubers of local and fuseau Jerusalem artichoke cultivars. Results indicated that Em, biocompost and biofert significantly increased plant height, number of main and lateral shoots and dry weight as well as the concentrations of Chl (a+b), carotenoids and total carbohydrates in the leaves. Moreover, dry matter, inulin, nitrogen, phosphorus and potassium of tubers showed a significant increases. The highest vegetative growth and tuber yield were obtained when Em, biocompost and biofert were applied. The respective increase in lateral shoots, Chl (a + b), carotenoids, average tuber weight and size were higher in local cultivar. Whereas fuseau cultivar tubers showed greater dry matter than those of local variety. The application of Em, biocompost and biofert fertilizers in both local and fuseau cultivar gave the relatively higher yield with good quality.