The aim of the present study to detect the population and frequency (%) of microorganism (bacteria, fungi and yeasts) in royal jelly samples. The data indicated that, there are no significant differences were remarked in the population of microorganisms between all the samples for bacteria, fungi and yeasts, where the mean number of population was 5.923, 1.38 and 7.85 colonies/sample for bacteria, fungi and yeasts respectively, in produced royal jelly from honeybee colonies, local royal jelly collected from Egyptian market and samples of imported royal jelly collected from Egyptian market, respectively. According to the isolation and identification procedures for detected royal jelly samples, four bacteria types (Clostridium botulinum, Bacillus cereus, Bacillus wakoensis and Micrococcus luteus), two fungi types (Aspergillusniger and Penicillium sp.) and one yeast type (Saccharomyces cerevisiae) were determined. The data also summarized that Clostridium botulinum was the most frequency compared with the other bacterial types, where the percentage of frequency was 1.8 – 2.5, 0.9 – 1.4, 0.4 – 0.6 and 1.5 – 2.0% for C. botulinum, B. cereus, B. wakoensis and Micrococcus luteus, respectively. Meanwhile, Penicillium sp. the most frequency compared with A. niger, where the percentage of frequency was 0.5 - 2.9 and 0.7 – 1.0 %, respectively, in produced royal jelly from honeybee colonies, local royal jelly samples collected from Egyptian market and imported royal jelly samples collected from Egyptian market respectively. Regarding the yeasts, the data also summarized that, S. cerevisiae was the most frequency in royal jelly that produced from  honeybee colonies (2.9%) followed by which local royal jelly samples collected from Egyptian market (2.1%) and imported royal jelly samples collected from Egyptian market (1.8%).

This work was carried out to study the effect of using some natural dietary supplements; i.e. powders of date palm pits and pollen, ginseng roots, moringa leaves and royal jelly which giving orally in capsule to improve endurance of weightlifter athletes throughout 12 weeks in camp of sport Championship Sector belongs to the Armed Forces, Ministry of Defense, Future City, Cairo, Egypt. Collecting data showed that the suggested dietary supplement capsule had the highest value of the antioxidant activity (96.3%) and significant increases in total phenolic and flavonoids content (52.42 & 63.1 mg/g, respectively). Also, the anthropometric measurements showed a significant increase in weight and height in males compared to females. likewise, waist and mid upper arm circumference had significant increases in males compared to females, Male weightlifter athletes had significant muscles mass bigger than female one, and the fat mass for male was assessed as ‘‘Fitness’’ which ranged between (14 and 17%) but the female had fat mass that assessed as ‘‘Athletes’’ which ranged between (14 and 20%). The weights lifting by male and female increased during three months, and also revealed a significant increase in the efficiency of males in lifting weights higher than in the females during the period of 12 weeks. Thus, the results indicated that by incorporating natural dietary supplement powders in suggesting capsule, it is possible to enhance the performance of weightlifter athletes.