Egyptian radish sterilized seeds were sprouted for 3 days using tap and saline water NaCl 2000 ppm to study sprout characters, chemical composition, protein fraction and safety aspects such as microbiological examinations and biogenic amines were investigated at 3 days old. The results indicated that sterilized seeds increased sprout growth compared with non-sterilized. Seeds sprouting increased in protein content, moister, ash and fibers while carbohydrate, lipid content and energy decrease in etiolated sprouts. Using saline water and seeds sterilization for sprouting increased albumin, globulin and glutenin fractionation but decreased prolamin compared with sprouts produced with tap water and without seed sterilization.  Using sterilization by 2% calcium hypochlorite of seeds before sprouting led to decreasing in total bacterial count compared with non-sterile seeds sprouts, the same was in total coliform, total yeast and total fungi counts. The sprouts product which washed with saline water was contain total bacterial count less than which washed by tap water. All sprouts under investigation were free from feacal coliform and all examined pathogenic microorganism under investigation like Staph. aureus, B. cereus and Salmonella spp.  Use sterilized seeds for sprouting caused big decrement on biogenic amines content of radish sprout. Radish sprout contain biogenic amines but it is lower than previous ranges, sprouts can be considered a safe food and germination of seeds either use tap or saline water.

In the current research, wheat grains were used to study the effect of grain soaking and sprouting using tap water and saline water (NaCl solution) on sprout growth, proximate analysis, minerals content, anti-nutritional and antioxidant compounds of sterilized grains (soaked for 0.33h) and soaked grains for imbibition (12h) and sprouted grain for 24h old. Results revealed that the longest radical of 24h old wheat sprout was observed at 2000 ppm NaCl, and shortest was observed at 4000 ppm NaCl. Soaked wheat grains (12h) for imbibition recorded the highest moisture content (10.2 to 10.9%) while soaked for 20 min (0.33h) in calcium hypochlorite for sterilization recorded medium content (8.8 to 9.9%) and the lowest one recorded in 24h  old wheat sprouts (6.9 to 7.2%). The low moisture content the high total carbohydrate, total fats and energy and vice versa. Soaked grains for sterilization period (0.33 h) and imbibition (12h) increased zinc (Zn), manganese (Mn) and calcium (Ca) while non-sterilized only potassium (K). Tap water increased sprout magnesium (Mg), and manganese (Mn) content while saline water increased sprout magnesium (Mg), and calcium (Ca) content. Grain sprouting was effective in reducing phytic acid, oxalate and alkaloids anti-nutrient in wheat sprouts especially when using sterilized grains. Soaking non sterilized grains for imbibition (12h) in saline water contained higher total phenol, flavonoids and total antioxidant. Etiolated wheat sprouts contained lower total  flavonoids and antioxidant compared with soaked grains in saline water.