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Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C
2021
Ahmed Seioudy | Esmat Abdalla | Alaa Zeidan | Farouk Khalil | Zienab Abdel-Salam | Magdy Badr | Maiada Allam
Total number of forty testes from twenty Sudani camels (Camelus dromedarius) were used in the present study (>5-10 years old and 500-600 kg body weight). The experimental work was executed to define the effect of green laser irradiation with short-wavelength 532 nm and continuous wave from a diode laser light with a total output power of 3 mW on epididymal camel spermatozoa quality at different exposure times of 0 (control, non-irradiated), 2, 4, 6, 8, and 10 min. Following irradiation, the percentages of motile spermatozoa, storagability, viability, and acrosomal damage were assessed of the epididymal camel spermatozoa stored at 5°C for 4 days. Epididymal spermatozoa was diluted with lactose-yolk-citrate (LYC) extender. The obtained results showed that the highest (P<0.05) value of the percentages of motile and storagability of spermatozoa was recorded with spermatozoa exposed to 6 min of laser irradiation and the lowest (P<0.05) value was recorded with the control group. Otherwise, the highest (P<0.05) value of the percentages of dead and acrosomal damage of spermatozoa was recorded with spermatozoa exposed to 10 min and the lowest (P<0.05) value was recorded with 2 min. The advancement of storage time at 5°C decreased (P<0.05) the percentages of motile and storagability of spermatozoa, while increased (P<0.05) the percentages of dead and acrosomal damage of spermatozoa during storage at 5°C for 4 days. Consequently, enhancing the artificial insemination program can be achieved using the laser irradiation which is considered a cost-effective technique for improving semen quality. The profitable effects of laser irradiation on epididymal camel spermatozoa quality raised the motile spermatozoa, storagability, livability, acrosomal integrity which consider an indicator to improve mitochondrial function which extends the survival of spermatozoa
Mostrar más [+] Menos [-]A Comparative Study Between Different Preservation Methods on The Viability of Some Yeast Cultures
2021
Reham Abdallah | Ibrahim Sayed Ahmed | amal hassan | hemmat Elshesheetawy
The influence of chilling, freezing, and dry-ing preservation methods on morphological properties and viability of four yeast cultures, isolated Saccharomyces boulardii (SB1 and SB2), Saccharomyces cerevisiae (SC), and Kluyveromyces lactis (KL), was studied. chilling preservation had no effect on yeast colony morphology parameters till the end of the preservation period in contrast with freez-ing and drying preservation methods. Drying preservation resulted in the highest viability decrement % of yeast cultures ranged between 59.38 to 40.21% as compared with other preservation methods. There is a noticeable re-duction in D values of preserved yeast cultures by drying, which varied from (33 to 26 day) in comparison to chilling and freezing preserva-tion that recorded (119 to 83 and 180 to 57 day), respectively. The obtained results re-vealed that chilling preservation under proper conditions, as well as freezing, is more prefer-able for the preservation of studied yeast cul-tures than that preserved yeast by the drying method.
Mostrar más [+] Menos [-]Viability and yield of Treated seeds with an oxygenated aqueous medium after storage
Boras, M.(Tishreen Univ., Lattakia (Egypt). Faculty of Agriculture)
Simultaneous selection for fiber yield and stability of crop performance in jute
Abo-Kaied, H.M.H. | El-Sweify, A.H.H.Ministry of Agriculture, Cairo (Egypt). Field Crops Research Inst.)