A pot study was conducted to investigate the potential of alfalfa for phytoremediation of soil artificially contaminated with different concentrations of Pb and Cd and their combinations. Harvested plants were divided and used for two purposes: (1) separation into roots and shoots, which were digested to determine N, P, K, Pb and Cd concentrations, and (2) the use of fresh shoots to prepare alfalfa extract representing each treatment. Subsequently, lettuce seedlings were grown in the same pots with the same soil and without adding fertilizers; the alfalfa shoot extract prepared from each treatment was added to the treatment itself. Lettuce crops harvested after 67 days from transplanting were divided into roots and shoots which were digested to determine the previously studied elements. In addition, soil samples were collected after harvesting alfalfa and lettuce plants and prepared for chemical analyses. Results showed that alfalfa is an effective accumulator plant for the phytoremediation of Pb- and Cd-contaminated soils. In addition, using the alfalfa shoot extract to fertilize lettuce crops was beneficial to their growth without any risk of translocation of heavy metals. Thus, we recommend adding alfalfa to crop rotations, especially where soils are contaminated with heavy metals.

A pot study was conducted to investigate the potential of alfalfa for phytoremediation of soil artificially contaminated with different concentrations of Pb and Cd and their combinations. Harvested plants were divided and used for two purposes: (1) separation into roots and shoots, which were digested to determine N, P, K, Pb and Cd concentrations, and (2) the use of fresh shoots to prepare alfalfa extract representing each treatment. Subsequently, lettuce seedlings were grown in the same pots with the same soil and without adding fertilizers; the alfalfa shoot extract prepared from each treatment was added to the treatment itself. Lettuce crops harvested after 67 days from transplanting were divided into roots and shoots which were digested to determine the previously studied elements. In addition, soil samples were collected after harvesting alfalfa and lettuce plants and prepared for chemical analyses. Results showed that alfalfa is an effective accumulator plant for the phytoremediation of Pb- and Cd-contaminated soils. In addition, using the alfalfa shoot extract to fertilize lettuce crops was beneficial to their growth without any risk of translocation of heavy metals. Thus, we recommend adding alfalfa to crop rotations, especially where soils are contaminated with heavy metals.

In contrast to the global countries, ectomycorrhizae members are not common in Egypt, however, they are very important in the afforestation of poor lands. Their occurrence in Pinus sp., Clerodendrum sp., and Eucalyptus sp. may help to explain why these trees are the most extensively dominant around the world, especially in Egypt. The identification and characterization of numerous ectomycorrhizal fungi often involve large morphological descriptions of sporocarps alone, which consequently, in some instances, raise arguments about the accuracy of these studies. The present work was achieved to isolate, identify, and characterize three ectomycorrhizal fungi from sporocarps combining morphological and molecular techniques. The morphological characteristics of tested species were assessed and compared to standard taxonomic literature. ITS-rDNA was utilized for molecular analysis using the universal fungal primers ITS1. Identification of these species was confirmed by comparing the sequences of amplified genomes of these species with respective species sequences in GenBank, followed by blast analysis.

In contrast to the global countries, ectomycorrhizae members are not common in Egypt, however, they are very important in the afforestation of poor lands. Their occurrence in Pinus sp., Clerodendrum sp., and Eucalyptus sp. may help to explain why these trees are the most extensively dominant around the world, especially in Egypt. The identification and characterization of numerous ectomycorrhizal fungi often involve large morphological descriptions of sporocarps alone, which consequently, in some instances, raise arguments about the accuracy of these studies. The present work was achieved to isolate, identify, and characterize three ectomycorrhizal fungi from sporocarps combining morphological and molecular techniques. The morphological characteristics of tested species were assessed and compared to standard taxonomic literature. ITS-rDNA was utilized for molecular analysis using the universal fungal primers ITS1. Identification of these species was confirmed by comparing the sequences of amplified genomes of these species with respective species sequences in GenBank, followed by blast analysis.

The main objectives of this study were to use rye grain in the diet of Turkish sheep as a substitute for barley grain and to understand the bio-logical value of rye in feeding lambs and weight gain. Animals were divided into 3 experimental groups with 3 replicates in each group. Control group (T1) received standard diet with barely grain while groups T2 and T3 fed on diets with substitution of 20 and 30% barely grain by rye grain. The results of the study showed that lambs fed on 20% rye seeds have a significant high-er weight gain as a results of better feed intake and feed conversion.

The main objectives of this study were to use rye grain in the diet of Turkish sheep as a substitute for barley grain and to understand the bio-logical value of rye in feeding lambs and weight gain. Animals were divided into 3 experimental groups with 3 replicates in each group. Control group (T1) received standard diet with barely grain while groups T2 and T3 fed on diets with substitution of 20 and 30% barely grain by rye grain. The results of the study showed that lambs fed on 20% rye seeds have a significant high-er weight gain as a results of better feed intake and feed conversion.

The study investigated the influence of traditional nitrogen, phosphorus and potassium (NPK) mineral fertilizers and nano-fertilizers on yield, and its attributes, and fiber characters of two Egyptian cotton varieties Giza 94 and Giza 96. The design of the experiment was a split- plot with four replicates. Results indicated that Giza 94 cultivar surpassed Giza 96 in agronomic characteristics, although Giza 96 exhibited the highest fiber reading. Nano-chitosan (NPK)-fertilization significantly improved the mean value of boll weight, seed yield, lint yield, lint percentage, upper half mean (UHM), fiber uniformity index, fiber tenacity, and Micronaire value in both seasons. Conversely, the most minimal values for the aforementioned characteristics were recorded with the control NPK fertilizer over the two seasons.

The study investigated the influence of traditional nitrogen, phosphorus and potassium (NPK) mineral fertilizers and nano-fertilizers on yield, and its attributes, and fiber characters of two Egyptian cotton varieties Giza 94 and Giza 96. The design of the experiment was a split- plot with four replicates. Results indicated that Giza 94 cultivar surpassed Giza 96 in agronomic characteristics, although Giza 96 exhibited the highest fiber reading. Nano-chitosan (NPK)-fertilization significantly improved the mean value of boll weight, seed yield, lint yield, lint percentage, upper half mean (UHM), fiber uniformity index, fiber tenacity, and Micronaire value in both seasons. Conversely, the most minimal values for the aforementioned characteristics were recorded with the control NPK fertilizer over the two seasons.

The importance of water footprint (WFP) is providing information related to water resource management, especially for countries that have water scarcity and rely on irrigation to enhance food security. A field experiment was conducted during two winter seasons in 2018, and 2019. The current study sought to evaluate the impacts of various nitrogen levels (1.N1: 120, 2.N2: 150, and 3.N3: 180 kg /fed) on potato production as well as the water footprint and water requirement. Vegetative characteristics, yield parameters, N contents, and climate data were measured. The obtained results are clarified that increasing the nitrogen rate up to 180 kg/fed led to increase the vegetative growth characteristics, yield parameters, and water footprint of potato crop. The most significant vegetative growth values were obtained using a 180 kg/fed nitrogen level followed by 150 kg/fed. The greater marketable tuber yield was recorded at 180 kg/fed with 18117 & 17753 Kg/fed values, followed by 150 kg/fed with 16864 and 16545 Kg/fed values for the first and second seasons respectively. The water footprint of potato in Egypt ranges from 237 to 267.8 for nitrogen levels of 120 kg /fed and 180 kg /fed.

The importance of water footprint (WFP) is providing information related to water resource management, especially for countries that have water scarcity and rely on irrigation to enhance food security. A field experiment was conducted during two winter seasons in 2018, and 2019. The current study sought to evaluate the impacts of various nitrogen levels (1.N1: 120, 2.N2: 150, and 3.N3: 180 kg /fed) on potato production as well as the water footprint and water requirement. Vegetative characteristics, yield parameters, N contents, and climate data were measured. The obtained results are clarified that increasing the nitrogen rate up to 180 kg/fed led to increase the vegetative growth characteristics, yield parameters, and water footprint of potato crop. The most significant vegetative growth values were obtained using a 180 kg/fed nitrogen level followed by 150 kg/fed. The greater marketable tuber yield was recorded at 180 kg/fed with 18117 & 17753 Kg/fed values, followed by 150 kg/fed with 16864 and 16545 Kg/fed values for the first and second seasons respectively. The water footprint of potato in Egypt ranges from 237 to 267.8 for nitrogen levels of 120 kg /fed and 180 kg /fed.

This study was conducted to investigate the effect of amino acid and vitamin foliar applications on improving iceberg lettuce (Lactuca sativa L.) production and quality during the summer. First, two hybrids (Patagonia and Bruma) were grown hydroponically in June 2018 and 2019. Then, for five consecutive weeks, plants were sprayed once a week with Stress Free (a commercial product comprising 0.5% vitamins [vitamin B complex/ascorbic acid] and 0.5% selenomethionine) at concentrations of 1.0, 2.0, and 3.0 g/L and Aminomad (20% free amino acids) at concentrations of 2.0, 3.0, and 4.0 cm3 /L. Investigations revealed that all plant growth aspects were positively improved with all foliar spray treatments than with the control. Furthermore, while mineral contents (nitrogen, phosphorus, potassium, calcium, and magnesium) showed positive responses to the applied treatments, the highest dose of amino acids showed the highest marketable and total yield effects among all foliar spray treatments with the Patagonia hybrid. However, the marketable and total yield of lettuce heads was lowest with the control treatment without spraying.

This study was conducted to investigate the effect of amino acid and vitamin foliar applications on improving iceberg lettuce (Lactuca sativa L.) production and quality during the summer. First, two hybrids (Patagonia and Bruma) were grown hydroponically in June 2018 and 2019. Then, for five consecutive weeks, plants were sprayed once a week with Stress Free (a commercial product comprising 0.5% vitamins [vitamin B complex/ascorbic acid] and 0.5% selenomethionine) at concentrations of 1.0, 2.0, and 3.0 g/L and Aminomad (20% free amino acids) at concentrations of 2.0, 3.0, and 4.0 cm3 /L. Investigations revealed that all plant growth aspects were positively improved with all foliar spray treatments than with the control. Furthermore, while mineral contents (nitrogen, phosphorus, potassium, calcium, and magnesium) showed positive responses to the applied treatments, the highest dose of amino acids showed the highest marketable and total yield effects among all foliar spray treatments with the Patagonia hybrid. However, the marketable and total yield of lettuce heads was lowest with the control treatment without spraying.

Soil temperature under mulching conditions has a significant impact on crop development, growth rate and other parameters. However, it is not included in the AquaCrop model. Thus, this study aims to improve the AquaCrop model performance for better simulation of soil mulches by considering the heat changing under mulch materials. The proposed modification is conducted through AquaCrop-Open Source software to identify the differences between the temperatures under the mulched soil and air temperatures. It will also help to describe them as additional heat units in specific growth stages. The field data used to evaluate the proposed model has previously been used to calibrate and validate the AquaCrop model in simulating melon growth under different irrigation treatments and soil mulching practices. The results show that the proposed model performs better than the original model in simulating mulched melon under different irrigation regimes. The root mean square error of biomass values was reduced under the modified model by 40%-75% under different irrigation treatments. Additionally, the coefficient of determination (R2) of the modified model slightly increased from the original one. Thus, the proposed model provides a more reliable and robust model.

Soil temperature under mulching conditions has a significant impact on crop development, growth rate and other parameters. However, it is not included in the AquaCrop model. Thus, this study aims to improve the AquaCrop model performance for better simulation of soil mulches by considering the heat changing under mulch materials. The proposed modification is conducted through AquaCrop-Open Source software to identify the differences between the temperatures under the mulched soil and air temperatures. It will also help to describe them as additional heat units in specific growth stages. The field data used to evaluate the proposed model has previously been used to calibrate and validate the AquaCrop model in simulating melon growth under different irrigation treatments and soil mulching practices. The results show that the proposed model performs better than the original model in simulating mulched melon under different irrigation regimes. The root mean square error of biomass values was reduced under the modified model by 40%-75% under different irrigation treatments. Additionally, the coefficient of determination (R2) of the modified model slightly increased from the original one. Thus, the proposed model provides a more reliable and robust model.

Liver toxicity is a common condition that can be induced by environmental pollutants. The present study explored the hepatoprotective activity of steroidal saponins extracted from the yam plant versus CC14-induced hepatotoxicity in albino rats. Twenty-five albino rats were classified into 5 groups. Rats of group (G1) were provided a basal diet and drinking water and served as un-treated controls. Other groups were administered CC14 orally twice a week at a dose of 400 mg/kg. The second group (G2) did not receive any further treatment and served as positive controls while rats in the groups G3, G4 and G5 were administered saponins (50,100 and 200 mg/kg body weight, respectively) for six weeks in the remaining groups. The hepatoprotective activity of saponins was assessed by measurement of liver enzymes, kidney function tests, malondialdehyde content, and antioxidant defense enzymes activities in serum of these rats. Saponins administration improved liver and renal function and significantly increased the activities of catalase, glutathione peroxidase (GSH-PX), glutathione reductase GSH-RD and superoxide dismutase SOD. These increases were linked to a considerable decrease in serum malondialdehyde levels, indicating that lipid peroxidation was being mitigated. Thus, the concentration of saponins (200 mg/kg) is the best concentration of protection against CC14-induced hepatic injury, improved liver and renal function, and reduced oxidative stress in rats.

Liver toxicity is a common condition that can be induced by environmental pollutants. The present study explored the hepatoprotective activity of steroidal saponins extracted from the yam plant versus CC14-induced hepatotoxicity in albino rats. Twenty-five albino rats were classified into 5 groups. Rats of group (G1) were provided a basal diet and drinking water and served as un-treated controls. Other groups were administered CC14 orally twice a week at a dose of 400 mg/kg. The second group (G2) did not receive any further treatment and served as positive controls while rats in the groups G3, G4 and G5 were administered saponins (50,100 and 200 mg/kg body weight, respectively) for six weeks in the remaining groups. The hepatoprotective activity of saponins was assessed by measurement of liver enzymes, kidney function tests, malondialdehyde content, and antioxidant defense enzymes activities in serum of these rats. Saponins administration improved liver and renal function and significantly increased the activities of catalase, glutathione peroxidase (GSH-PX), glutathione reductase GSH-RD and superoxide dismutase SOD. These increases were linked to a considerable decrease in serum malondialdehyde levels, indicating that lipid peroxidation was being mitigated. Thus, the concentration of saponins (200 mg/kg) is the best concentration of protection against CC14-induced hepatic injury, improved liver and renal function, and reduced oxidative stress in rats.

The scarcity of fresh water is a primary problem in remote regions. Thus, an economical and related water distillation solar still coupled with a solar collector was designed and studied experimentally. This study aims to investigate and evaluate the performance of an active single-slope solar still (ASSSS) coupled with a solar evacuated tube collector (ETC) as the water heater. The results showed that the average distillate productivity of ASSSS combined with U pipe solar (ETC) was 1.085 l/m2 in December 2020, but 3.12 l/m2 in August 2021. These quantities of the water distiller were higher than those of a passive solar distiller with a single slope (PSSSS). The average value of the water temperature increased using the ASSSS coupled with ETC in August 2021 at 79.1°C, whereas it was 71°C in August 2021 for PSSSS. These results indicate that the ASSSS is more effective than the PSSSS.

The scarcity of fresh water is a primary problem in remote regions. Thus, an economical and related water distillation solar still coupled with a solar collector was designed and studied experimentally. This study aims to investigate and evaluate the performance of an active single-slope solar still (ASSSS) coupled with a solar evacuated tube collector (ETC) as the water heater. The results showed that the average distillate productivity of ASSSS combined with U pipe solar (ETC) was 1.085 l/m2 in December 2020, but 3.12 l/m2 in August 2021. These quantities of the water distiller were higher than those of a passive solar distiller with a single slope (PSSSS). The average value of the water temperature increased using the ASSSS coupled with ETC in August 2021 at 79.1°C, whereas it was 71°C in August 2021 for PSSSS. These results indicate that the ASSSS is more effective than the PSSSS.

Two field experiments were conducted during in 2019/20 and 2020/21 seasons to study the impact of integration among mineral and nanoparticle nitrogen (N) fertilizer levels on yield traits and chemical characters of some canola genotypes grown under salt stress conditions. Four treatments of N fertilization (190 kg N/ha as recommended dose; 50% of the recommended, 95 kg N/ha+nano nitrogen (5 L/ha); 25 % of the recommended, 47.5 kg N/ha+nano-nitrogen (5 L/ha), and nano-nitrogen (rate of 5 L/ha). canola genotypes (Trabber, Agamax, and Serw4) performance were assessed under three levels of saline irrigation water (control, 2000, and 4000 mg L-1). Results showed that increasing salinity levels up to 4000 mg L-1 led to decreasing in all studied yield parameters compared with those of control (tap water). Trabber genotype excelled significantly in most of the yield characteristics. Integration between nanoscale and mineral nitrogen fertilizer, i.e. 95 kg N/ha+5 nano N L/ha) showed superiority over all applied N treatments, recording the highest values. It could be concluded that since application of 95 kg N/ha+5 nano N L/ha exploiting the nano form of N saves about 50% of applied nitrogen in canola under saline conditions. Accordingly, nanoparticles help the environmental pollution to be reduced.

Two field experiments were conducted during in 2019/20 and 2020/21 seasons to study the impact of integration among mineral and nanoparticle nitrogen (N) fertilizer levels on yield traits and chemical characters of some canola genotypes grown under salt stress conditions. Four treatments of N fertilization (190 kg N/ha as recommended dose; 50% of the recommended, 95 kg N/ha+nano nitrogen (5 L/ha); 25 % of the recommended, 47.5 kg N/ha+nano-nitrogen (5 L/ha), and nano-nitrogen (rate of 5 L/ha). canola genotypes (Trabber, Agamax, and Serw4) performance were assessed under three levels of saline irrigation water (control, 2000, and 4000 mg L-1). Results showed that increasing salinity levels up to 4000 mg L-1 led to decreasing in all studied yield parameters compared with those of control (tap water). Trabber genotype excelled significantly in most of the yield characteristics. Integration between nanoscale and mineral nitrogen fertilizer, i.e. 95 kg N/ha+5 nano N L/ha) showed superiority over all applied N treatments, recording the highest values. It could be concluded that since application of 95 kg N/ha+5 nano N L/ha exploiting the nano form of N saves about 50% of applied nitrogen in canola under saline conditions. Accordingly, nanoparticles help the environmental pollution to be reduced.