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MICROBIAL PRODUCTION OF THERMOALKALIPHILIC ENZYMES FROM EL-KHORMA GOVERNORATE FOR APPLICATION IN BIODETERGENT TECHNOLOGY
2011
Detergents are an undetectable source of pollution, which hidden in most of our daily activities. Detergents could cause harmful effects before they are completely degraded. It is wise to reduce the amount of detergents that usually used by invention new bio-friendly formula contains efficient enzymes such as protease. Screening studies were carried out for one hundred and fifty bacterial isolates with respect to their ability to produce protease(s), after growing on slaughter house wastes (SHW) isolated from El-Khorma governorate,Taif, Kingdom of Saudi Arabia (KSA) at 55ºC, and pH 9. The most potent thermophilic bacterial isolate concerning of alkaline thermostable protease(s) production was identified as Bacillus licheniformis EGT50. Alkaline thermostable proteases productivity by the most potent bacterial isolate was affected by substrate concentrations (solid substrate), carbon source, nitrogen source, amino acid supplements, incubation temperature, incubation period, and inoculum size. Maximum both enzymes production by B. licheniformis EGT50 was obtained on SHW concentrations, 7.5 %; galactose; diammonium hydrogen phosphate; arginine at 55°C for 72 h. when inoculated by 0.5 ml. The protease production under all optimal conditions was increased many folds from 563.68 to 17825 U/ml (31 fold). The purification fold of B. licheniformis EGT50 alkaline thermostable protease increased to 394.7 after applying Sephadex G200 column chromatography techniques. The enzyme productivity of protease has been determined and the result proved the possibility to use the crude and purified enzymes in biodetergent technology
Mostrar más [+] Menos [-]ULTRASTRUCTURE OF TWO GENUS Przhevalskiana silenus (Brauer, 1858) AND Cochliomyia hominivorax (Coquerel, 1858) WHICH AFFECTS GOATS BY MYASIS IN SAUDI ARABIA
2011
Sara Aljubran | Souad Alsaqabi
Ultrastructure study revealed two types of fly larvae Przhevalskiana silenus (Brauer, 1858) and Cochliomyia hominivorax (Coquerel, 1858) that causes myasis in Goats in Saudi Arabia, The study recorded that the exact composition of these two genus showed differences in morphological characteristics, which cannot be identified using an optical microscope. All previous studies recorded on the same region never before carried out ultrastracture studied.
Mostrar más [+] Menos [-]IDENTIFICATION OF THE GENETIC RELATIONSHIP OF ALMOND SEED WASP Eurytoma Amygdali End IN MID AND SOUTHERN OF SYRIA
2011
Ghali Roda S. | Alkassis W. | Lawand S.
The almond seed wasp Eurytoma amygdali Enderlein (Eurytoma amygdale End) is an important insect on almonds Amygdalus spin its plantig regions, causing a large loss in the production. The relationship between the adults of almond seed wasp in five regions: mid Syria (Homs), Southern region (Al Qunaitera, Swedaa), and the countryside of Damascus (Beet Tema,, Deer Ateah) was studied by ISSR. The results indicated that the females from Homs formed a separated cluster, and the females from Beet Tema and Deer Ateah were the most closed to each other,also the female from Al Qunaitera and Swedaa were closed to each other. It was noted that the males separated into two clusters, the first one contained the males from Homs and Swedaa, and the second contained the males from Beet Tema, Al Qunaitera and Deer Ateah.
Mostrar más [+] Menos [-]Taxonomic evaluation using pollen grain sculpture and seed coat characters of 11 taxa of genus hibiscus, malvaceae, in Egypt
2011
El-Kholy, M.A. | Kasem, W.T. | Mabrouk, A.S.
Pollen grain morphology and seed coat characters of 11 cultivars belonging to two species of genus Hibiscus (Family Malvaceae) namely H. esculentus (H. Abelmoschus) and H. sabdariffa were investigated. This study was carried out using light microscope (LM) and Scanning Electron Microscopy (SEM). Pollen morphology of this genus is fairly uniform. Generally radially symmetrical apolar, mostly spheroidal, pantoporate. Seed exomorphic characters revealed four types of ornamentations; reticulate, ocealate, foveolate and ruminate. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was employed to characterize those taxa. Thirty one bands of seed protein profiles have been constructed from the gel. The produced dendrograms were analyzed by STATISCA program using UPGMA clustering method showed a close affinity among the seven H. esculentus cultivars and the four H. sabdariffa cultivars.
Mostrar más [+] Menos [-]Anti-tumor activities and oil constituents of zingiber officinale, Zingiberaceae
2011
El-Swaify, Z.A. | Abd El-Kawy, A.M.
The phytochemical studies on Zingiber officinale rhizomes revealed that it contains traces of flavonoid, carbohydrates, tannins, steroles or terpenoids and it is free from alkaloids .Assay of essential oil of Zingiber officinale rhizome was also carried out. The alcoholic extract of the plant rhizome, was examined against five tumor cell lines, BHK-2, HEPG-2, MCF-7, HCT-2 and HEP-2, using SRB. assay. All cell lines were growth inhibited in a dose dependent manner after exposure to the plant extract. The antitumor activity of the plant extract using E.A.C. method, showed a high activity against mice tumor.
Mostrar más [+] Menos [-]Cellulase productrion two fungal strains isolated from Taif in Saudi Arabia
2011
Nasr, S.A. | Hussein, N.A. | Abuo zaid, A.A. | Al-Salemi, F.A
Among 17 fungal isolates isolated from soil of El-hawia, El-hada, El-kaym and Karwa in Taif governorate in Saudi Arabia, two isolates showed high efficacy in producing cellulases enzymes. They were identified to be Altemaria altemata and Aspergillus wentii. Some factors such as carbon and wheat bran as a raw material, nitrogen, pH and incubation temperature were investigated. Results indicated that glucose and cellulose were the most effective as a carbon source while, urea was the best nitrogen source for cellulases production. Initial pH 5.0 and incubation temperatures at 25 or 35°C achieved high cellulases production.
Mostrar más [+] Menos [-]Influence of green-house cover on potential evapotranspiration and cucumber water requirements
2011
Hashem. F.A. | Medany, M.A. | Abd El-Moniem, E.M.
The experiment was conducted through two successive seasons of 2008 and 2009 at ElBosaily farm, EI-Behira governorate at the North Coastal of the Nile Delta, in Egypt. This work aimed to study the effect of three green-house covers (polyethylene sheet, white and black net) and three irrigation levels [80%, 100% and 120% of the potential evapotranspiration estimated according to class A pan equation (ET,)], applied by drip irrigation system, on plant growth and crop yield of Cucumber (Cucumis sativus L.. cv. Reda F1). The experimental design was split- plot with three replicates. The results showed that white net green-house cover optimized growth and yield of cucumber plant. White net cover treatment recorded the highest vegetative growth (plant height, number of leaves, total leaves area, total fresh and dry weights), and significantly increased total yield. The highest vegetative growth was obtained by 100% ET, compared to 80 and 120% ETo treatments. The interaction among water treatment and green-house cover indicated the highest vegetative growth and crop yield were obtained under white net cover wi1th 100% ET0,.
Mostrar más [+] Menos [-]Micropropagation of damask rose, Rosa damascena
2011
Alsemaan, T. | Albatal, N. | Almaarri, Kh.
This study aimed to establishing a protocol for producing in-vitro plants of Rosa Damascene. The culture was conducted at the Commission of Biotechnology in Damascus. Four types of explants were cultured on MS medium and some factors affecting culture were examined. The results showed that no viruses were observed, the lateral buds were superior over other explants, then the lateral microcuttings, after that, the apical microcuttings, and, finally, the shoot tips. The highest multiplication rate was observed at the hormonal combinations of (benzyl adenine BA 3mg/I with indole-3-acetic acid IAA 0.1 mg/1), and the highest elongation average were observed at ( IAA 0.1 mg/I with BA 2-6mg/I) or ( indole-3-butyric acid (IBA) 0.1 with (BA) 5-6 mg/I). The transferring was positively effective. The highest rooting percentage was observed when naphthalene acetic acid NAA or IBA were used. (Berlite: peatmoss, 1:1) was the best growing medium for hardening.
Mostrar más [+] Menos [-]Modernity of farmers a study of two villages in Minufiya Governorate
2011
Keneber, Kh.A. | Shahin, A.S.
.The results revealed that The highest proportion of farmers was between 28 and 60 years. Mean values for family size in the different study areas were 5.49, the main occupation of respondents was agriculture (60%) A large share of farmers (70%) received formal education, while (30%) of farmers did not receive any formal education. Farmers in the study area were small-scale farmers, it showed that the average size of land owned was one feddan, the average level of living was 12.19, the average annual gross farm income in the study area was 11362 L_.E per household. All farmers in the study areas were members of the agricultural cooperative, which exist in all villages. Membership of these cooperatives was mandatory for those farmers who had reasonable access to it; 51.5% of farmers have a medium level of cosmopoliteness, 37% have a high level. More than 75% of farmers were falling in the medium level of communication with agricultural information, more than 65% of farmers had a medium level of aspiration, only 1.5% of farmers had a high level of aspiration, The highest proportion of farmers was a medium degree of filiations to community. The results showed that non of the respondents from the sample size had a high level of agricultural modernity, 76.5% had a medium level and 23.5 % only had low level. The coefficients of the logistic model used to investigate factors affecting the modernity of farmers. The chi-square test showed the level of significance of the parameters included in the model (at P 0.01). Modernity of farmers was correctly predicted for 77.5% of the farmers. The factors that contribute to modernity of farmers according to the Wald chi-square statistic were in order of importance: degree of affiliation, cosmopoliteness, family size, farm size, and aspiration level.
Mostrar más [+] Menos [-]Taxonomic study of two genus in lice linognathus africanus kellogg, and paine, 1911 bovicola, damalinia,ovis 1781 by scanning electronic microscope, sem
2011
Aljubran, S.A. | Alsaqabi, S.M.
This study presents the Mallophaga and Anoplura lice which affects farm animals (camels sheep and goats), a local area east of the Saudi Arabia of different ages, races, classes. Study the classification of lice was recorded as using the optical microscope and electronic scanning. The study shows scanning electron microscopy micro-structure of the species: Bovicola (Damalinia) ovis, Linognathus africanus and the study showed the installation of a portfolio of head lice, and the distribution of filament head, chest and abdomen, as shown over the centuries, the difference in the form of remote sensing and Swallows in thesexuallice, the first study in this area.
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