This study analyzed data from 5,518 lactations involving 1,749 Friesian cows raised in two experimental herds between 1992 and 2022.  The study aim was to estimate the genetic parameters for longevity traits using a multiple-trait animal model, including age at first calving (AFC), lifetime (LT), lifetime days in milk (LTDIM), total lactations (TL) and lifetime milk yield (LTMY). On average, cows lived for 80.4 months, with 41% of their lifetime spent in milk production. They averaged 3.2 lactations, starting at 32.7 months. Heritability estimates for longevity traits were 0.09 to 0.12 suggesting limited direct selection potential. Strong genetic and phenotypic correlations (0.88–0.96) were observed between lifespan traits and total milk production. Similar correlations were found between partial lifetime milk yield traits for the first three lactations and total lifetime milk yield (0.44 to 0.97). Genetic correlations between lifetime traits were consistently high (0.88 to 0.99), mirroring phenotypic correlations. Notably, heritability estimates for partial performance traits increased with more lactations considered, and correlations between partial and lifetime traits were improved with additional data. These findings suggest the feasibility of early indirect selection for longevity through correlated responses in early performance traits, particularly early lactation milk production, to enhance overall lifetime productivity.

This study analyzed data from 5,518 lactations involving 1,749 Friesian cows raised in two experimental herds between 1992 and 2022.  The study aim was to estimate the genetic parameters for longevity traits using a multiple-trait animal model, including age at first calving (AFC), lifetime (LT), lifetime days in milk (LTDIM), total lactations (TL) and lifetime milk yield (LTMY). On average, cows lived for 80.4 months, with 41% of their lifetime spent in milk production. They averaged 3.2 lactations, starting at 32.7 months. Heritability estimates for longevity traits were 0.09 to 0.12 suggesting limited direct selection potential. Strong genetic and phenotypic correlations (0.88–0.96) were observed between lifespan traits and total milk production. Similar correlations were found between partial lifetime milk yield traits for the first three lactations and total lifetime milk yield (0.44 to 0.97). Genetic correlations between lifetime traits were consistently high (0.88 to 0.99), mirroring phenotypic correlations. Notably, heritability estimates for partial performance traits increased with more lactations considered, and correlations between partial and lifetime traits were improved with additional data. These findings suggest the feasibility of early indirect selection for longevity through correlated responses in early performance traits, particularly early lactation milk production, to enhance overall lifetime productivity.

Climate change poses a significant challenge to agriculture while barley is an essential and crucial crop worldwide. This study evaluated the drought stress tolerance of 25 barley genotypes. A field experiment was carried out to investigate agronomical traits, such as plant height at 110 days (PH110) and spike length (SL), in response to different surface irrigation treatments. Subsequently, 15 barley genotypes were chosen for the second experiment which aimed to examine the impact of physiological stress generated by polyethylene glycol-6000. Several biological metrics, including seedling vigor index (SVI), and drought tolerance index (DTI), were quantified. Ultimately, six SSR primers were used to analyze the genetic diversity between different barley genotypes. The findings demonstrated that the G1, G2, and G6 genotypes were tolerant but G5, G9, and G14 were susceptible. The primers Bmag0603, EBmac0849, and Bmag770 were polymorphic. This study provides valuable initial insights into the drought resistance of various barley genotypes, highlighting the genetic diversity and potential for breeding drought-tolerant varieties. We suggest expanding the sample size and incorporating a broader range of environmental conditions in future studies to validate these findings. Additionally, the identified genetic markers could be further explored and utilized in breeding programs.

Climate change poses a significant challenge to agriculture while barley is an essential and crucial crop worldwide. This study evaluated the drought stress tolerance of 25 barley genotypes. A field experiment was carried out to investigate agronomical traits, such as plant height at 110 days (PH110) and spike length (SL), in response to different surface irrigation treatments. Subsequently, 15 barley genotypes were chosen for the second experiment which aimed to examine the impact of physiological stress generated by polyethylene glycol-6000. Several biological metrics, including seedling vigor index (SVI), and drought tolerance index (DTI), were quantified. Ultimately, six SSR primers were used to analyze the genetic diversity between different barley genotypes. The findings demonstrated that the G1, G2, and G6 genotypes were tolerant but G5, G9, and G14 were susceptible. The primers Bmag0603, EBmac0849, and Bmag770 were polymorphic. This study provides valuable initial insights into the drought resistance of various barley genotypes, highlighting the genetic diversity and potential for breeding drought-tolerant varieties. We suggest expanding the sample size and incorporating a broader range of environmental conditions in future studies to validate these findings. Additionally, the identified genetic markers could be further explored and utilized in breeding programs.

This study was conducted to examine the effects of the foliar application of moringa and germinated fenugreek extracts on the growth and biochemical parameters of wheatgrass grown under saline conditions. Aqueous moringa extract (MLE) was used at concentrations of 1.25, 0.83, 0.62 and 0.31%, whereas fenugreek extract (GFE) was used at concentrations of 0.25, 0.20, 0.15 and 0.10%. Optimum germination conditions were investigated under normal and NaCl stress (2000 ppm) conditions. Exposure to saline stress significantly reduced seed germination and grass yield as well as shoot and seedling fresh and dry weights. Ideal germination conditions were MLE 0.31% and GFE 0.15%, resulting in increased growth traits compared with the other concentrations and control. Foliar application of MLE or GFE improved growth traits, total antioxidants, phenols, Ca, Mg, K, Mn, Cu, Zn and Fe in the presence or absence of NaCl. In addition, wheatgrass powder was used to replace wheat flour in developing biscuits at 5, 10 and 15% levels. Wheatgrass showed high feasibility for formulating nutrient-rich biscuits with acceptable sensory qualities. These findings provide useful information for enhancing the nutrients in biscuits, with potential use as a natural source of bioactive compounds in functional food products.

This study was conducted to examine the effects of the foliar application of moringa and germinated fenugreek extracts on the growth and biochemical parameters of wheatgrass grown under saline conditions. Aqueous moringa extract (MLE) was used at concentrations of 1.25, 0.83, 0.62 and 0.31%, whereas fenugreek extract (GFE) was used at concentrations of 0.25, 0.20, 0.15 and 0.10%. Optimum germination conditions were investigated under normal and NaCl stress (2000 ppm) conditions. Exposure to saline stress significantly reduced seed germination and grass yield as well as shoot and seedling fresh and dry weights. Ideal germination conditions were MLE 0.31% and GFE 0.15%, resulting in increased growth traits compared with the other concentrations and control. Foliar application of MLE or GFE improved growth traits, total antioxidants, phenols, Ca, Mg, K, Mn, Cu, Zn and Fe in the presence or absence of NaCl. In addition, wheatgrass powder was used to replace wheat flour in developing biscuits at 5, 10 and 15% levels. Wheatgrass showed high feasibility for formulating nutrient-rich biscuits with acceptable sensory qualities. These findings provide useful information for enhancing the nutrients in biscuits, with potential use as a natural source of bioactive compounds in functional food products.

Genetic diversity (GD) helps in the selection of valuable parents which is the key to improving heterosis breeding and thus GD within the collected or derived germplasm should be explored. The present study aims to quantify the GD of restorer lines of rapeseeds based on seed yield components. An experiment was conducted in a Randomized Complete Block Design with three replications. The first four principal components explained 35%, 24%, 16% and 10% of total variance, respectively. PC1 exhibited a positive relationship with branches/plant, pods/plant, thousand-seed weight, and seed yield/plant. The PC1 and PC2, projected branches/plant and pods/plant, showed a positive correlation with seed yield/plant. There were also positive correlations among plant height and days with first blooming and 100% flowering and maturity. Cluster study revealed that the restorer lines were clustered into 4 clusters where Tori was the only one (cluster 4) that did not coalesce with the rest of the R lines. Clusters 1-3 consist of 10, 13 and 11 R lines. Based on the above findings, diverse genotypes can be selected for the development of high-yielding variety through hybridization.

Genetic diversity (GD) helps in the selection of valuable parents which is the key to improving heterosis breeding and thus GD within the collected or derived germplasm should be explored. The present study aims to quantify the GD of restorer lines of rapeseeds based on seed yield components. An experiment was conducted in a Randomized Complete Block Design with three replications. The first four principal components explained 35%, 24%, 16% and 10% of total variance, respectively. PC1 exhibited a positive relationship with branches/plant, pods/plant, thousand-seed weight, and seed yield/plant. The PC1 and PC2, projected branches/plant and pods/plant, showed a positive correlation with seed yield/plant. There were also positive correlations among plant height and days with first blooming and 100% flowering and maturity. Cluster study revealed that the restorer lines were clustered into 4 clusters where Tori was the only one (cluster 4) that did not coalesce with the rest of the R lines. Clusters 1-3 consist of 10, 13 and 11 R lines. Based on the above findings, diverse genotypes can be selected for the development of high-yielding variety through hybridization.

Atriplex halimus is a widely distributed species in the Mediterranean coastal areas and can grow under saline and non-saline habitats. Plant leaves were collected from two habitats; non-saline (1.14 dSm-1) and saline (30.63 dSm-1) at Borg Alarab area along the Northwestern coast of Egypt to investigate the ecomorphological and physiological behavior variations of A. halimus in relation to different habitat. leaf area, specific leaf area (SLA), water content, chl a, chl b, total chl, carotenoids, Na+, K+, Ca++ and Cl- contents were measured. A. halimus leaves generate adaptive changes as plastic responses to salinity by the decrease in leaf area, SLA, chl a, chl b, totat chl and Cl and increase in Na+ , phenylalanine ammonia lyase (PAL) specific activity and total phenols content.

Atriplex halimus is a widely distributed species in the Mediterranean coastal areas and can grow under saline and non-saline habitats. Plant leaves were collected from two habitats; non-saline (1.14 dSm-1) and saline (30.63 dSm-1) at Borg Alarab area along the Northwestern coast of Egypt to investigate the ecomorphological and physiological behavior variations of A. halimus in relation to different habitat. leaf area, specific leaf area (SLA), water content, chl a, chl b, total chl, carotenoids, Na+, K+, Ca++ and Cl- contents were measured. A. halimus leaves generate adaptive changes as plastic responses to salinity by the decrease in leaf area, SLA, chl a, chl b, totat chl and Cl and increase in Na+ , phenylalanine ammonia lyase (PAL) specific activity and total phenols content.

This study was conducted to determine the impact of sodium chloride (2000 ppm) and salicylic acid (SA) concentrations (0, 50, 100, 150, and 200 ppm) on the nutritional value and germination of radish microgreens. The experiment was based on a completely random design with three replicates. Results indicated that applying SA at 50 and 100 ppm mitigates the negative effects of salinity, even better than higher concentrations, on the nutritional value and bioactive contents of radish microgreens where SA at 50 ppm lowered the sodium contents from 1.75 to 1.10 ppm in addition to increasing the moisture content. Applying SA at 50 and 100 ppm caused a noticeable increase in carbohydrates, proteins, fats, fibers and energy under unstressed condition. In addition, SA at 50 ppm caused a significant increase in oxalic and malic acid contents under both normal and salinity conditions while SA at 100 ppm significantly enhanced the levels of total phenols, flavonoids and antioxidants under salinity conditions. This practical study highlights the significant role of salicylic acid, at 50 and 100 ppm, in increasing the levels of phytochemicals and minerals, hence improving the nutritional value of microgreen radishes.

This study was conducted to determine the impact of sodium chloride (2000 ppm) and salicylic acid (SA) concentrations (0, 50, 100, 150, and 200 ppm) on the nutritional value and germination of radish microgreens. The experiment was based on a completely random design with three replicates. Results indicated that applying SA at 50 and 100 ppm mitigates the negative effects of salinity, even better than higher concentrations, on the nutritional value and bioactive contents of radish microgreens where SA at 50 ppm lowered the sodium contents from 1.75 to 1.10 ppm in addition to increasing the moisture content. Applying SA at 50 and 100 ppm caused a noticeable increase in carbohydrates, proteins, fats, fibers and energy under unstressed condition. In addition, SA at 50 ppm caused a significant increase in oxalic and malic acid contents under both normal and salinity conditions while SA at 100 ppm significantly enhanced the levels of total phenols, flavonoids and antioxidants under salinity conditions. This practical study highlights the significant role of salicylic acid, at 50 and 100 ppm, in increasing the levels of phytochemicals and minerals, hence improving the nutritional value of microgreen radishes.

The Tomato Chlorosis Criniviruses (ToCV) and Tomato yellow leaf curl Geminivirus (TYLCV), which naturally infecting and limited to the phloem, have caused a drastic reduction in tomato yield. The current study aims to determine the incidence of single and mixed viruses using biological, serological and molecular PCR methods in natural tomato plants. The incidence of mixed infection was found more frequently, followed by ToCV and TYLCV (42.3, 28.8, 17.8 in 2020 and 49.1, 29.7 and 19.1% in 2021, respectively). ToCV causes chlorosis, TYLCV causes leaf curl and yellowing, while mixed ToCV & TYLCV cause progress symptoms. By using the heat shock protein 70 (HSP70) and coat protein (CP) genes, ToCV and TYLCV isolates could be identified. These isolates were recorded in GenBank under accession codes "ON951644.1" and "OP265136.1" respectively. Host plants responded differently to severe and common disease density between ToCV and TYLCV. The transmitted whitefly could distinguish between ToCV and TYLCV within 15-20 minutes of the acquisition period. ToCV increases in fields with high whitefly populations, requiring further research to understand effects and reduce harm.

The Tomato Chlorosis Criniviruses (ToCV) and Tomato yellow leaf curl Geminivirus (TYLCV), which naturally infecting and limited to the phloem, have caused a drastic reduction in tomato yield. The current study aims to determine the incidence of single and mixed viruses using biological, serological and molecular PCR methods in natural tomato plants. The incidence of mixed infection was found more frequently, followed by ToCV and TYLCV (42.3, 28.8, 17.8 in 2020 and 49.1, 29.7 and 19.1% in 2021, respectively). ToCV causes chlorosis, TYLCV causes leaf curl and yellowing, while mixed ToCV & TYLCV cause progress symptoms. By using the heat shock protein 70 (HSP70) and coat protein (CP) genes, ToCV and TYLCV isolates could be identified. These isolates were recorded in GenBank under accession codes "ON951644.1" and "OP265136.1" respectively. Host plants responded differently to severe and common disease density between ToCV and TYLCV. The transmitted whitefly could distinguish between ToCV and TYLCV within 15-20 minutes of the acquisition period. ToCV increases in fields with high whitefly populations, requiring further research to understand effects and reduce harm.

Mulberry trees suffer from many pest infestations. To control these pests, some natural compounds such as Techno Oil and Top 9 and some other chemicals such as Mospilan® and Chinook® were used. Mulberry trees, Morus nigra were treated with three different concentrations of each of Chinook, Techno Oil® and Top 9® to reduce infestation with Icerya sp. and Ferrisia virgate. A high reduction rate of more than 90% occurred when using these pesticides. On the other hand, the corrected mortality of mulberry silkworm larvae was 93.3%, 0.0% and 6.7% respectively after 8 days of treatment. When treating mulberry trees Morus alba with two different concentrations of each of pesticides; Mospilan and Techno Oil against whitefly, the average rate of infestation reduction was 100% and 94.94%, respectively. The corrected mortality of mulberry silkworms' larvae was 100% and 0.0% after 8 days of treatment respectively. This study proved that natural compounds could be used safely instead of chemical pesticides to reduce the pest population without affecting the life of silkworm larvae or cocoon properties.

Mulberry trees suffer from many pest infestations. To control these pests, some natural compounds such as Techno Oil and Top 9 and some other chemicals such as Mospilan® and Chinook® were used. Mulberry trees, Morus nigra were treated with three different concentrations of each of Chinook, Techno Oil® and Top 9® to reduce infestation with Icerya sp. and Ferrisia virgate. A high reduction rate of more than 90% occurred when using these pesticides. On the other hand, the corrected mortality of mulberry silkworm larvae was 93.3%, 0.0% and 6.7% respectively after 8 days of treatment. When treating mulberry trees Morus alba with two different concentrations of each of pesticides; Mospilan and Techno Oil against whitefly, the average rate of infestation reduction was 100% and 94.94%, respectively. The corrected mortality of mulberry silkworms' larvae was 100% and 0.0% after 8 days of treatment respectively. This study proved that natural compounds could be used safely instead of chemical pesticides to reduce the pest population without affecting the life of silkworm larvae or cocoon properties.

Heat stress is an environmental problem that can negatively impact tomato productivity by reducing fruit setting and disrupting pollen development. The present study aims to understand the molecular mechanism of heat tolerance induced by L-ascorbic acid. Plants were grown in the summer of 2022 (day and night temperatures were 34.4°C and 25.2°C respectively). The plants were divided into two groups: one was sprayed with L-ascorbic acid (0.5 mM) and the other served as a control. Growth parameters, antioxidant enzymes, proline, hydrogen peroxide, and malondialdehyde levels in the leaves were determined. Additionally, a molecular docking allowed understanding the interaction between the antioxidant enzymes and L-ascorbic acid. Results indicated that L-ascorbic acid increased antioxidant enzyme activities (catalase, peroxidase, ascorbate peroxidase, polyphenol oxidase), proline content, fruit setting and growth indicators (shoot and root fresh and dry weight). It also reduced hydrogen peroxide and malondialdehyde levels in tomato leaves. Furthermore, the in-silico analysis revealed that L-ascorbic acid binding energies toward antioxidant enzymes were similar to those of known activators. In conclusion, foliar application of L-ascorbic acid (0.5 mM) effectively mitigated heat stress effects, activating antioxidant enzymes and eliminating reactive oxygen species, ultimately resulting in increased fruit setting.

Heat stress is an environmental problem that can negatively impact tomato productivity by reducing fruit setting and disrupting pollen development. The present study aims to understand the molecular mechanism of heat tolerance induced by L-ascorbic acid. Plants were grown in the summer of 2022 (day and night temperatures were 34.4°C and 25.2°C respectively). The plants were divided into two groups: one was sprayed with L-ascorbic acid (0.5 mM) and the other served as a control. Growth parameters, antioxidant enzymes, proline, hydrogen peroxide, and malondialdehyde levels in the leaves were determined. Additionally, a molecular docking allowed understanding the interaction between the antioxidant enzymes and L-ascorbic acid. Results indicated that L-ascorbic acid increased antioxidant enzyme activities (catalase, peroxidase, ascorbate peroxidase, polyphenol oxidase), proline content, fruit setting and growth indicators (shoot and root fresh and dry weight). It also reduced hydrogen peroxide and malondialdehyde levels in tomato leaves. Furthermore, the in-silico analysis revealed that L-ascorbic acid binding energies toward antioxidant enzymes were similar to those of known activators. In conclusion, foliar application of L-ascorbic acid (0.5 mM) effectively mitigated heat stress effects, activating antioxidant enzymes and eliminating reactive oxygen species, ultimately resulting in increased fruit setting.

This study was conducted to investigate the effects of organic, NPK, and biofertilizers on the yield of two cowpea varieties grown under arid land conditions. Two cultivars of cowpea (Karim-7 and Dokki-331) were evaluated using different fertilizer types. The fertilizers examined were organic (farmyard manure (FYM) and chicken manure (CHM)) and biofertilizers (effective microorganisms (EM1) and technology of smart fertilizer (TS)) in addition to NPK treatments (NPK (50 kg/fed), NPK (100 kg/fed), EM1 (15 m3.fed-1) + NPK (50 kg/fed), TS (15 m3.fed-1) + NPK (50 kg/fed). A randomized complete block design (RCBD) with 16 treatments and three replications was used to set up the experiment. The measured yield parameters were seed number/plant, pod length, seed number/pod, pod number/plant, dry yield/plant, dry yield/m2, bio yield, pod weight, 100-seed weight, and grain yield. The results cleared that the cultivar dokki-331 under EM1 biofertilizer + NPK (50 kg/fed) combination treatment was the superior practice for increasing all studied traits.

This study was conducted to investigate the effects of organic, NPK, and biofertilizers on the yield of two cowpea varieties grown under arid land conditions. Two cultivars of cowpea (Karim-7 and Dokki-331) were evaluated using different fertilizer types. The fertilizers examined were organic (farmyard manure (FYM) and chicken manure (CHM)) and biofertilizers (effective microorganisms (EM1) and technology of smart fertilizer (TS)) in addition to NPK treatments (NPK (50 kg/fed), NPK (100 kg/fed), EM1 (15 m3.fed-1) + NPK (50 kg/fed), TS (15 m3.fed-1) + NPK (50 kg/fed). A randomized complete block design (RCBD) with 16 treatments and three replications was used to set up the experiment. The measured yield parameters were seed number/plant, pod length, seed number/pod, pod number/plant, dry yield/plant, dry yield/m2, bio yield, pod weight, 100-seed weight, and grain yield. The results cleared that the cultivar dokki-331 under EM1 biofertilizer + NPK (50 kg/fed) combination treatment was the superior practice for increasing all studied traits.