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DETECTION OF MUTAGENICITY IN SOME CURED MEAT PRODUCTS USING AMES TEST
2018
Kariman Hassan | R. Mahmmod | M. Khallaf | Lamyaa Sayed
The use of food additives must be under control specially with the improvement of many diseases such as cancer disease which became the most threaten disease all over the world, although there had become more health aware and medical culture, many unhealthy food products are being consumed increasely, so it became very important to study some food product’s mutagenicity. Detecting mutagenicity with short term assay with high percentage sensitivity are specifications available at Ames test with the mutated Salmonella typhimiurium strains and the reverse growth of the mutated bacteria was an indicator to the sample tested mutagenicity .The aim of this study is to evaluate the effect of adding sodium nitrite salt with various levels on mutagenicity in two of processed meat products (pastirma and luncheon) using Ames test. The results gave in the tested samples sign of mutagenicity at low concentrations and high reverse growth at higher concentrations , sodium nitrite extract gave highest mutagenicity at 10% (1.5 ml dose ) concentration , pastirma extract gave highest mutagenicity at 10% concentration (2 ml dose ) and luncheon extract gave highest mutagenicity at 100% concentration.
Mostrar más [+] Menos [-]ECONOMIC ASSESSMENT OF THE RURAL DEVELOPMENT PROJECT PHASE AS ONE OF THE FOREIGN FUNDED PROJECTS
2018
A. Ahmed | W. Mugahid | A. Abdel Maqsoud
This research was generally aimed at conducting an economic study to evaluate the Beheira Governorate Rural Development Project Phase II, because of the availability of data necessary to carry out the ordinary economic and financial assessment, As one of the leading rural development projects in Egypt financed by of the Bank and the African Development Fund, where research has been aimed at studying the vulnerabilities to avoid them in the future, and strengths to sustain and develop it and increase its efficiency, and this can only be achieved through a systematic scientific assessment of the project. It was one of the most important findings of the research that the project was characterized by rates of performance exceeding the target of the project in most of its activities. In addition to the feasibility of the project from the point of view of the financial and economic assessment, Despite the fact that the project is an agricultural development and most of its activities are not for profit, Despite the fact that the project is an agricultural development and most of its activities are not for profit, it achieved an internal rate of return of about 12.3% and achieved a positive net present value of about 7.7 million pounds, and The project has succeeded in recovering its capital in about two and a half years. The project has added value to beneficiaries of about 23.6 million pounds while achieving a national added value of about 10.5 million pounds and the social surplus rate is about 10. 2 million pounds, However, it is noted that the project spent a lot of money in the training activity, due to the limited education and lack of expertise among the beneficiaries and the lack of skilled labor, which led the project to pump a large part of the project funding for training activities.
Mostrar más [+] Menos [-]EFFECT OF SPRAYING WASHINGTON NAVEL ORANGE TREES WITH SELENIUM ON VEGETATIVE GROWTH, PRODUCTIVITY AND FRUIT QUALITY
2018
B. Bakr | A. El-Gazzar | Noha Mansour | M. Fawzy
ve seasons, 2012 and 2013 in a private orchard in Barshom vallage located at El-Kalubia Governorate Egypt. Washington Navel Orange trees (Citrus Sinensis) budded on sour orange rootstock (Citrus aurantium, L.) were 7- years- old and planted at 5 x 5 meters under basin irrigation system. This investigation aimed to study the effect of selenium (Se) levels (0, 20, 40, 80 and 160 ppm) as foliar spray on growth, yield, fruit quality, leaf mineral content and enzymes activity of Washington Navel orange trees. The obtained data showed that, selenium had a significant promotive effect on growth, yield, fruit quality, leaf mineral content and enzymes activity of Washington Navel orange trees. Results indicated that treatment of Se at 40 ppm gave the highest significant results, whereas, treatment 160 ppm gave the highest fruit selenium content.
Mostrar más [+] Menos [-]IMPACT OF FLAXSEED INCLUSION IN DAMASCUS GOAT'S RATION ON DIGESTIBILITY, SOME BLOOD PARAMETERS AND REPRODUCTIVE PERFORMANCE
2018
M. Meteab | H. El-Sayed | Abeer EL-Essawy | M. Nassar | N. El-Bordeny
Feeding flaxseed to dairy animals improve milk production and reproductive performance. So, the objective of our study was to investigate effect of inclusion flaxseed in Damascus goat's ration on its reproductive performance. Twenty-four Damascus goats (31.40 ±0.50 kg weight and 2-3 years old) were divided into three groups (randomly, eight animals each). The basic diet of control group (T1) consisted of 56.67% concentrate feed mixture (CFM) and 33.33% Alfalfa hay, while the groups T2 and T3 supplemented with 10% full fat soya, 5% flaxseed + 5% full fat soya and 10% flaxseed, respectively. Inclusion flaxseed in goat’s ration at rate of 5 and 10% resulted in significant increase in all nutrients digestibility, except nitrogen free extract digestibility (NFE) as well as the nutritive values as total digestible nutrients (TDN). Dry matter intakes (DMI) of the different experimental groups were comparable. Goats in T3 recorded the highest does conceived, twinning, conception and kidding rates % as well as total kids born, than other groups (T1 and T2). On the hand T3 recorded the lowest aborted does and barren does (Zero) compared to T1 and T2. Animals of T3 recorded significant higher of total protein, globulin, Albumin and urea concentration than T1 and T2. Concerning lipids profile, the animals of T3 recorded significant lower triglycerides, cholesterol and Low-density lipoprotein concentration than those of T1 and T2. While the animals of T3 recorded significant higher High-density lipoprotein concentration compared to T2 and T1. The body weight of does is not significantly affected by any of three treatments. In conclusion, flaxseed supplementations in Damascus goat's diets during the gestation period lead to improve total tract digestibility, reduced blood lipids and normalize renal and hepatic functions and improved its reproductive performance, especially those fed on diets containing 10% flaxseed.
Mostrar más [+] Menos [-]MICROPROPAGATION AND EVALUATION OF GENETIC STABILITY OF FOXGLOVE TREE (Paulownia tomentosa)
2018
K. Hassan | A. Hosni | M. Hewidy | A. Abd El razik
This reasearch work aimed to fine-tune micropropagation of Paulownia tomentosa in addition to assessing the genetic stability of in vitro raised clones from it. Paulownia tomentosa explants were surface sterilized using clorox (commercial bleach 5.25% sodium hypochlorite) at 10, 20, 25 and 30% + 0.5 g/l mercuric chloride (HgCl2) at different duration times, i.e. 10, 15, 20 and 25 min. In the multiplication stage, shoots were transferred to MS medium at ¾ strength containing BAP and Kin each at (0, 0.5, 1, 2, and 4 mg/l). Whereas, the rooting medium was MS medium at ¾ strength with IBA and NAA treatments each at 0, 0.5, 1, 2, and 4 mg/l. Sterilized explant with 30% Clorox for 20 min recorded highest survival percentage. The treatment of Kin at 4 mg/l gave higher significant shoot length. Whereas BAP application at 2 and 4 mg/l gave highest significant value of both shoot number and leaf number. Both IBA and NAA at 0.5 or 1 mg/l gave highest significant root number/shoot. Whereas, auxin at 4 mg/l gave highest significant root lengths. Young plantlets resulted from in vitro were acclimitized successfully in a mixture of peat moss: perlit (2: 1) by volume that showed 85.93% survival. The genetic stability of in vitro raised Paulownia tomentosa clones was assessed by using intersimple sequence repeats (ISSRs) markers. All of the three ISSR primers screened, produced clear, reproducible and scorable bands. The molecular size of Polymerase Chain reaction (PCR) products generated 22 fragments by these ISSR ranged from ≈460 to18660 bp. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant, indicating 100% similarity. This confirmed the true to type nature of the in vitro raised clones.
Mostrar más [+] Menos [-]HYPOCHOLESTEROLEMIC EFFECT OF SAPONIN EXTRACTS IN EXPERIMENTAL ANIMALS
2018
Amany Ali | M. Tawfik | M. Hikal | M. Tag El-Din
Hypercholesterolemia was induced by feeding of rats on a high cholesterol diet (HCD) that contains cholesterol (1%), bile salts (0.25%) and coconut butter (15%) to evaluate the protective effect of steroidal saponins extracted from fenugreek and asparagus, and triterpenoidal saponins extracted from soapwort and licorice. The rats were divided into 6 groups, and the first one was fed on a basal diet and served as a negative control group. The second group of rats received HCD without any plant extract and served as a positive control group. The other four groups of rats were fed on HCD plus the plant extracts separately at a dose of 250 mg/kg/day during the experiment period (6 weeks). The protective effect of various saponin extracts were monitored through assays of total cholesterol (TC) and triglycerides (TG) in blood, liver tissues and feces of the rats as well as other blood analyses and histological examinations of liver tissues. The data indicated a significant (P<0.05) increase in the levels of TC, TG, low density lipoprotein (LDL), very low density lipoprotein (VLDL), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyltransferase (GGT) and glucose, and a significant (P<0.05) decrease in the level of high density lipoprotein (HDL) in addition to insignificant (P<0.05) differences in the levels of total bilirubin (TB), total protein (TP), urea, creatinine and uric acid in serum of hypercholesterolemic rats (the positive control) compared to the negative control. The protective effect of various saponin extracts were established by lowering the levels of TC and TG, and obtaining the other biochemical parameters near to their normal values in serum of rats fed on these plant extracts. The data also indicate that TC and TG decreased significantly (P<0.05) in liver tissues of the rats treated with various saponin extracts compared to the positive control. Conversely, the levels of TC and TG increased significantly (P<0.05) in feces of the rats treated with various saponin extracts compared to the negative and positive controls. Histological examinations showed lower content of fats in liver tissues of the rats treated with various saponin extracts compared to the positive control. Both of steroidal and triterpenoidal saponins exhibited approximately the same efficiency in their protective effects against hypercholesterolemia. The hypocholesterolemic effect of saponin extracts may be due to the inhibition of cholesterol absorption in the intestine which led to increase of cholesterol excretion in the feces.
Mostrar más [+] Menos [-]THE OPTIMUM SIZE OF MANGO FARMS IN ISMAILIA
2018
A. Barakat | M. El-Sintrissi | M. Abdel-Fattah
This study aims to study the estimation of production cost functions as it reflects the relationship between total costs and actual production. The main economic indicators that reflect the extent to which the agricultural production units achieved economic efficiency in their use of the productive resources involved in the production process as well as the extent to which they are achieved These units of maximizing the profits of the agricultural product, as well as the study of production costs and the associated indicators are important both on the productivity unit or at the national level, which can be used in the design and analysis of price policies and help the user Farmers in their productive decision-making, which would encourage producers to continue production or stop production. As well as to determine the volume of production that maximizes profits and thus achieve economic efficiency of the use of productive elements.
Mostrar más [+] Menos [-]ESTIMATED GENE EXPRESSION IN MEAT QUALITY TRAIT UNDER STRESS CONDITIONS FOR TWO BROILER STRAINS
2018
Kareman Nasser | lamiaa Radwan | M. Mahrous | Neima Alsenosy | A. Farag
The objective of this study was to estimated gene expression in breast muscles of two broiler strains with used to Myoustatin gene in marketing age (5 weeks of age) under heat stress and compared to control group for 1 hour daily for 7 days in Shalakan farm of Faculty of Agriculture, Ain Shams University, broiler chicks (150 chicks in each strains) of 1 day of age were used in this study. So, the measurements were taken (respiratory rate, rectal temperature and estimated gene expression). The results obtained the gene expression of Myostatin gene in muscle breast was observed highest in Ross strain compared with Indian River strain under heat stress, but no different between strains ( Ross and Indian River) in control group. However, respiratory rate of Ross and Indian River under heat at 5 week of age showed that the Indian River strain was high significant at 5 week of age compared to Ross ones. As shown that rectal temperature of Ross and Indian River were a high significant effect of treatment (heat group was higher from control group) and not a significant of strain.The present experiment was conducted to estimated gene expression on meat quality traits of different tropical stress conditions in different broiler strains during 7 days and 5 weeks of age. Introgressions some major genes likes myostatin gene in muscle breast into broiler improve under the heat stress.
Mostrar más [+] Menos [-]EFFECT OF SUBSTITUTION OF WHEAT FLOUR WITH QUINOA FLOUR ON QUALITY OF PAN BREAD AND BISCUIT
2018
E. Moawad | I. Rizk | Y. Kishk | M. Youssif
This study was carried out to investigate the effect of partial substitution (5, 10 and15%) of wheat flour (72% ext.) by whole meal quinoa flour (QF) on quality parameter of pan bread. QF contained the highest percentage of protein, Lipids, ash and crude fiber. Also, QF contained the highest amount of essential amino acids such as (threonine, methionine, lysine and histidine). From the results, it could be seen that, water absorption and degree of softening increased by increasing the substitution levels, but stability, resistance to extensions and energy of dough decreased. The addition of QF adversely affected on the specific volume of pan bread. The lightness (L*) and yellowness (b*) of pan bread decreased, but redness (a*) increased gradually by increasing QF. Evaluation of the organoleptic properties of pan bread revealed that no significant differences (P≥0.05) between control sample and bread samples contained QF for taste and summitry form. The bread contained QF had higher score for crust color, pore size and overall acceptability than control sample. Hardness (g) of pan bread increased and springiness decreased gradually during storage of bread at (25˚c ±2). The rate of staling of bread contained QF lower than control sample. Gluten – free biscuit made from 100%QF contained the highest percentage of protein, lipids, ash and crude fiber compared to that of corn and rice – quinoa composite flour. The addition of corn and rice flour adversely affected on the thickness, diameter and spread ratio. The highest spread ratio was noticed in the biscuit made from 100% QF. It is worth mentioning that the biscuit made from 100 % QF or that of corn and rice – quinoa composite flour gave the biscuit with sensory acceptable. On the other hand, it could be noticed to that the biscuit contained high level of quinoa flour was darker in compared to another samples.
Mostrar más [+] Menos [-]DETECTION OF MICROORGANISMS (BACTERIA, FUNGI AND YEASTS) IN ROYAL JELLY
2018
Zeinab Ashour | M. Ali | Sawsan Abdelmegeed | K. Amin
The aim of the present study to detect the population and frequency (%) of microorganism (bacteria, fungi and yeasts) in royal jelly samples. The data indicated that, there are no significant differences were remarked in the population of microorganisms between all the samples for bacteria, fungi and yeasts, where the mean number of population was 5.923, 1.38 and 7.85 colonies/sample for bacteria, fungi and yeasts respectively, in produced royal jelly from honeybee colonies, local royal jelly collected from Egyptian market and samples of imported royal jelly collected from Egyptian market, respectively. According to the isolation and identification procedures for detected royal jelly samples, four bacteria types (Clostridium botulinum, Bacillus cereus, Bacillus wakoensis and Micrococcus luteus), two fungi types (Aspergillusniger and Penicillium sp.) and one yeast type (Saccharomyces cerevisiae) were determined. The data also summarized that Clostridium botulinum was the most frequency compared with the other bacterial types, where the percentage of frequency was 1.8 – 2.5, 0.9 – 1.4, 0.4 – 0.6 and 1.5 – 2.0% for C. botulinum, B. cereus, B. wakoensis and Micrococcus luteus, respectively. Meanwhile, Penicillium sp. the most frequency compared with A. niger, where the percentage of frequency was 0.5 - 2.9 and 0.7 – 1.0 %, respectively, in produced royal jelly from honeybee colonies, local royal jelly samples collected from Egyptian market and imported royal jelly samples collected from Egyptian market respectively. Regarding the yeasts, the data also summarized that, S. cerevisiae was the most frequency in royal jelly that produced from honeybee colonies (2.9%) followed by which local royal jelly samples collected from Egyptian market (2.1%) and imported royal jelly samples collected from Egyptian market (1.8%).
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