Q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium Coxiella burnetii. The aim of this study was to investigate the presence of C. burnetii infection in aborted sheep in eastern Turkey using PCR. A total of 200 fetuses were collected from aborted sheep belonging to 200 herds in different locations in the eastern part of Turkey. Foetal organ samples such as liver, spleen, lung and stomach were taken and the DNA was purified from two hundred pooled samples. PCR analysis of C. burnetii presence in infected organs was performed, and 4 samples (2%) were found positive. In addition, the pooled organ suspensions were inoculated to embryonated chicken eggs, and PCR analysis of yolk sacs showed C. burnetii DNA in 5 samples (2.5%). This study shows that C. burnetii infection has an important role in sheep abortions in eastern Anatolia region.

Meat sheep production in most countries relies on grazing and profitability (gross margin per ewe and per year) is low. Profitability is in part based on the level of numeric productivity (number of lambs produced per ewe and per year). Numeric productivity depends on fecundity and survival rates of ewes and lambs. These survival rates are extremely variable from one farm to another. These variations are detrimental to sustainability of the farms. We will study the mortality rates of ewes and lambs and other health related traits, taken as indicator in meat sheep farms in different locations-plain or semi-mountain areas (34 farms) in relation to gross margin taken as an indicator of economical sustainability as well as other markers of sustainability. We constructed a method for evaluating sustainability based on the above mentioned parameters in the field of production, animal health, and economics. It was based on two step procedure: first, selection of farm parameters using clustering methods, second ranking farms on sustainability using principal component analysis with an orientation obtained by the incorporation in the analysis of two virtual farms (high and low sustainability) constructed on a choice of sustainability for each parameter. This choice of sustainability is flexible and dependant on the views of stakeholders and may modify the ranking of farms. The scale of sustainability we proposed was tested in relation to the management of the farm (organic or conventional) and location (plain or semi-mountain areas): the semi-mountain farms were more sustainable than plain farms and organic farms were often, but not always, more sustainable than conventional farms. The method can be easily applied to any animal production.

To measure florfenicol concentrations in ovine tear fluid after IM and SC administration and determine minimum inhibitory concentrations (MICs) of florfenicol against field isolates of Mycoplasma organisms potentially involved in infectious keratoconjunctivitis. ANIMALS: 9 healthy adult Lacaune ewes. PROCEDURES: Animals received an IM and SC administration of florfenicol (20 mg/kg) in a 2-way crossover design. Samples of blood and tear fluid were collected before and for 24 hours after administration. Concentrations of florfenicol in plasma and tear fluid were measured via high-performance liquid chromatography. The MIC of florfenicol for various Mycoplasma strains cultured from sheep and goats was determined via an agar dilution method. RESULTS: Mean florfenicol concentration in tear fluid for the 24-hour period was significantly higher after IM administration (0.70 μg/mL) than after SC administration (0.22 μg/mL) and was maintained for a longer duration. The lacrimal fluid-to-plasma concentration ratio was not different between the 2 routes of administration, with mean values of 40.2% and 32.5% after IM and SC administration, respectively. The MIC for Mycoplasma agalactiae, Mycoplasma conjunctivae, and Mycoplasma mycoides isolates ranged from 0.5 to 8 μg of florfenicol/mL. Two strains of M agalactiae could be considered resistant to florfenicol. CONCLUSIONS AND CLINICAL RELEVANCE: Florfenicol readily penetrated the preocular tear fluid of sheep after IM and SC administration. For both routes of administration, doses > 20 mg/kg would be necessary to achieve tear fluid concentrations of florfenicol greater than the MICs for most strains of Mycoplasma organisms.

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In Burkina Faso, rural livestock farmers in arid and semi-arid areas rely heavily on woody plant resources such as Acacia nilotica and Acacia raddiana to satisfy the needs of small ruminant rearing. The assessment of the availability and the use of these two species are therefore essential to better manage them sustainably for their preservation. The survey carried out in the area showed that the questioned farmers exploited the leaves and pods of the two Acacia for the needs of the ruminants. 100% of the farmers exploit A. raddiana only for the feeding of small ruminants. However, A. nilotica was exploited for animal feed and health. The most commonly reported treatments are Foot and Mouth Disease (98.7%) followed by diarrhea (73.7%) and foot wounds (51.2%). In order to compare the results of the survey, a botanical inventory was carried out in the area and the results showed that A. raddiana is more available compared to A. nilotica. Also, other local species such as Faidherbia albida, Acacia senegal, Balanites aegyptiaca and Acacia siberiana are also exploited for the needs of small ruminant rearing in the study area. The most important constraints were excessive cutting, drought and overexploitation that negatively impact their survival. Data on these constraints provide the basis for a campaign to raise awareness among herders' communities to preserve these plants to sustainably improve the productivity of small ruminants and consequently farmer’s income.

Necrotoxigenic Escherichia coli (NTEC) are associated with intestinal and extraintestinal diseases in animals and human beings and produce Cytotoxic Necrotizing Factor 1 (CNF1) or 2 (CNF2). Fourty-three NTEC1, 42 NTEC2, and 32 CNF-negative isolates from cattle were tested by colony DNA hybridization, by plasmid DNA hybridization and by PCR assays for the presence of DNA sequences homologous to the operons coding for fimbrial (PAP/PRS, SFA/FIC, and F17) and afimbrial (AFA/Dr) adhesins of extraintestinal E. coli. Most NTEC1 isolates hybridized with the PAP probes and either the SFA probe (37%) or the AFA probes (49%). Most NTEC2 isolates, in contrast, hybridized with the F17 probe (45%), the AFA probes (19%), or the F17 and AFA probes (22%). A probe-positive plasmid was identified in each of the 19 NTEC2 isolates studied. They all hybridized with the CNF2 toxin probe (Vir plasmids) and most of them with the F17 (6 plasmids) or AFA (7 plasmids) probes. PCR amplification was obtained with 6 of the 11 NTEC isolates tested for the papGII/prsG genes; with all 5 NTEC isolates tested for the sfa and related operons; but with none of the 18 NTEC isolates tested for the afa and related operons. pap-, sfa-, and afa-related sequences are thus present in NTEC isolates from cattle in addition to f17-related operons and may code for adhesins corresponding to specific colonization factors. f17- and afa-related sequences can be located on the Vir plasmids along with the cnf2 gene. Existence of new variants of the AFA/Dr family is evident from the negative results of this family-specific PCR assay.

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