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Evaluation of surface plasmon field-enhanced fluorescence spectroscopy for rapid measurement of progesterone concentration in bitches
2021
Fontbonne, Alain | Maenhoudt, Cindy | Thoumire, Sandra | Roos, Juliette | Niewiadomska, Zuzanna | Robiteau, Guillaume | Rousselière, Edwige | Buronfosse, Thierry | VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) | Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP) ; VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
International audience | OBJECTIVE To compare progesterone (P4) concentrations measured with surface plasmon field-enhanced fluorescence spectroscopy (SPFS) and chemiluminescence immunoassay (CLIA) in serum and plasma samples of client-owned bitches of various ages and breeds and to determine reference ranges for P4 concentrations at various stages of the estrous cycle. SAMPLES 102 serum samples and 104 plasma samples. PROCEDURES In experiment 1, 1 aliquot each of serum and plasma was analyzed for P4 concentration by use of SPFS incorporated in a veterinary-specific point-of-care immunologic analyzer and CLIA. In experiment 2, serum collected from bitches in various stages of the estrous cycle was analyzed for P4 concentration by use of SPFS to establish reference ranges for each stage. RESULTS In experiment 1, P4 concentrations measured by SPFS and CLIA were highly correlated (serum, r = 0.966; plasma, r = 0.968). In experiment 2, ranges of serum basal (proestrous) P4 concentrations (n = 114) and P4 concentrations at the estimated time of ovulation (76), during pregnancy or diestrus (107), and during the prepartum period (50) measured with SPFS were 0.42 to 1.46 ng/mL, 3.69 to 7.85 ng/mL, 11.73 to 28.24 ng/mL, and 1.54 to 3.22 ng/mL, respectively. CONCLUSIONS AND CLINICAL RELEVANCE Because serum and plasma P4 concentrations measured with SPFS were highly correlated with those measured with CLIA and ranges of serum P4 concentrations measured with SPFS for each of phase of the estrous cycle were well-defined for the large sample size, veterinarians may be able to accurately use this veterinary-specific point-of-care immunologic analyzer with SPFS methodology to determine P4 concentrations of bitches in their daily practice.
Mostrar más [+] Menos [-]Personality in young horses and ponies evaluated during breeding shows: phenotypic link with jumping competition results
2021
Vidament, Marianne | Lansade, Léa | Danvy, Sophie | Priest, Bernard Dumont Saint | Sabbagh, Margot | Ricard, Anne | Institut Français du Cheval et de l'Equitation [Saumur] (IFCE) | Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Génétique Animale et Biologie Intégrative (GABI) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Corrigendum to “Personality in young horses and ponies evaluated during breeding shows: Phenotypic link with jumping competition results” Journal of Veterinary Behavior 44 (2021) 1–11/ JVEB_2018_190 paru dans Journal of Veterinary Behavior Volume 66, August 2023, Page 35 DOI:10.1016/j.jveb.2023.06.011 WOS:001068066200001 | International audience | Animal personality, the result of temperament being modulated by life events, is an important factor to be considered when breeding and using domestic horses. In the breeding of sport horses, personality appears as a secondary trait in selection objectives after competition performance. Moreover, the per-sonality trait of fearfulness may be viewed as a risk factor for riders. This study aimed to estimate the variability of personality characteristics measured during breeding shows and their phenotypic correla-tion with performance in jumping competitions. Data for personality characteristics were recorded during 67 breeding shows in France on 876 jumping horses, 424 jumping ponies and 45 leisure ponies aged 2 or 3 years. Their behavior was assessed during 1) customary rounds (CR) of breeding shows (conforma-tion, free jumping and height measurement at withers) and 2) specific tests (ST) conducted in-hand that measured fearfulness (novel object, novel surface and suddenness tests) and tactile sensitivity. Not all the animals were evaluated on all the behavior tests. Jumping performances from 4 to 7 years old were recorded for 724 of the horses and for 313 of the ponies in official competitions specific for horses or ponies. Environmental effects were estimated using general linear model taking into account breeding show, age and sex. The breeding show effect was significant on 23 out of 28 characteristics. Age and sex influenced approximately one third of the characteristics: younger animals were more fearful; males moved and whinnied more; geldings appeared slightly more fearful during ST. Jumping performances were mostly independent of personality characteristics. In horses, performances were phenotypically pos-itively linked with 3 characteristics during jumping CR (whinnies ( P = 0.05), main gait when entering ( P = 0.02), evasive behaviors ( P = 0.03)) and with posture during conformation evaluation CR ( P = 0.04). In ponies, jumping performances were phenotypically positively linked only with whinnies: during CR of height measurement ( P = 0.02) and during all ST ( P = 0.01). As no main fear variables were significantly related to jumping performances in the two studied populations, it seems that less fearful horses and ponies may perform well in show jumping.
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