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Detection of African swine fever viral antigens in paraffin-embedded tissues by use of immunohistologic methods and polyclonal antibodies
1992
Martínez Fernández, Ángel | Pérez, J. | Carrasco, L. | Sierra, M.A. | Sanchez-Vizcaino, M. | Jover, A.
Tissues obtained from pigs inoculated with African swine fever virus (ASFV), fixed by vascular perfusion using glutaraldehyde, and embedded in paraffin or araldite were used for an immunohistologic electron microscopic study. To detect ASFV antigens, 4 methods were used on paraffin sections with or without pretreatment of the tissues. Use of biotinylated anti-ASFV antiserum combined with avidin -biotin complex and peroxidase proved to be the most suitable method, and antigen was detected in tissues infected with 2 ASF viruses of different virulence. Use of the glutaraldehyde fixation method should ensure optimal morphologic (structural and ultrastructural) data while allowing an immunohistologic study, and add to knowledge of the pathogenesis of ASF.
Mostrar más [+] Menos [-]Effect of administration with fat-free diet and dietary iron on polyunsaturated fatty acid, vitamin E and their derivatives in male rats
1990
Huh, R.S. | Kim, Y.H. | Do, J.C. | Lee, Y.H. | Choi, Y.S. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine) | Kim, S.H. (Korea Research Institute of Chemical Technology, Taejon (Korea Republic))
