This study was carried out to investigate the branch and distribution of Nervus facialis of the Korean native goat. The observation was made by dissection of embalmed cadavers of ten Korean native goats. N. facialis arose from the ventrolateral surface of the medulla oblongata. In the facial canal, N. facialis gave off N. petrosus major, N. stapedius and Chorda tympani. N. petrosus major arose from Ganglion geniculi, passed through the pterygoid canal and terminated in Ganglion pterygopalatium. Chorda tympani joined N. lingualis at the lateral surface of the internal pterygoid muscle.

The anatomical structure of pelvic limb of thirty-one adult Korean native goats (Body weight : 14-17kg) was observed after skeletal preparation, and the osteometry was performed in each bone. The pelvic limb of the Korean native goat was composed of the hip bone, femur, patella, tibia, fibula, tarsal bones, metatarsal bone, phalanges and sesamoid bones. The hip bone consisted of the ilium, ischium and pubis which fused each other. The gluteal surface of the ilium was directed dorsolaterally. The tuber sacrale and tuber coxae were formed at the dorsal apex and ventrolateral part of the iliac wing, respectively. The lesser ischiatic notch was deeper than the greater one. The ischiatic tubercles were triangular and consisted of the dorsal, lateral and caudal ischiatic tubercles. The left and right hip bone indexes were 67.08, 66.20, the acetabular indexes were 93.78 and 92.10 and the obturator formen indexes were 53.84 and 54.77, respectively. In femur, both of the greater and lesser trochanter were well developed but the third trochanter was not observed. The left and right femur indexes were 26.55 and 26.14, head indexes were 81.66 and 81.49 and the trochlear-epicondyle indexes were 42.47 and 41.63, respectively. The patella was observed as an isosceles triangle with base lying proximal and the cranial surface was more convex. The tibial shaft was sigmoid (S-) shaped and the popliteal notch was deep. There was a large nutrient formen at the cranial aspect of the cranial intercondylar area. The tibial indexes were 22.09 (left) and 21.10 (right). The proximal extremity of the fibula was fused with the lateral condyle of the tibial but the distal one was observed independently as the malleolar bone.

This study was undertaken to obtain basic data of the sternal development in Korean native cattle from the earliest sternal formation to the ossification using histological and histochemical methods. Thirty three sterna were collected from a series of embryos and fetuses ranging from 11 to 225mm (estimated age 37-120 days) in crown rump length. The bilateral sternal bars were observed in the 2nd group (CRL 21-30mm) of Korean cattle embryos. Those bars initiated to be fused in the 3rd group (CRL 31-40mm) and completed in the 7th group (CRL 71-80mm). The ossification centers were detected in the 8th group (CRL 81-90mm) also bilateral ossification centers were found in the same group. The typical epiphyseal plates, endochondral bone and calcium deposit were found in the 9th group (CRL 91-100mm). Osteocytes, osteoblasts, osteoclasts and myeloid cells appeared in ossification centers in the 10th group (more than CRL 101mm). The alcianophility responded markedly in the 9th group that was decreased and showed slightly positive reaction in territorial matrix of the 10th group. Marked positive reaction to PAS was observed in bony trabeculae in the 10th group. The positive reaction to calcium deposit by trichrome stain was observed initially in the hypertrophied zone of epiphyseal plate in the 9th group and was conspicuous in the calcified zone of epiphyseal plate in the 10th group. The 1st positive reaction to the von Kossa stain was observed in the 9th group.

We have developed in situ hybridization(ISH) technique for rapid diagnosis of canine distemper(CD) which is the major infectious disease in dogs. In our experiment, we rapidly detected distribution of the specific canine distemper viral genome without disrupting morphology of tissues of cells. Two oligonucleotide probes for ISH were synthesized chemically and labelled 5' end with nonisotopic biotin by DNA synthesizer. The whole procedures of ISH was completed within 1~2 hours using the Microcapillary action system. On histological study, typical cytoplasmic or intranuclear inclusion bodies wer observed in the trachea, bronchiole, brain, and urinary bladder with the presence of prominent red positive signals on ISH, indicating specific CDV genome from the paraffin-embedded tissues of infected 13 cases. The results showed ISH can be used as a rapid and effective diagnostic method for diagnosis of CD.

Morphometrical analysis of chicken Crytosporidium baileyi in various stages of life cycle in the bursa of Fabricius wer carried out by electron microscope to establish a differential point for identification of C baileyi. By avidin-biotin complex method, protozoans of the bursa of Favricius were identified Cryptosporidium spp. The size and area on each developmental stages of C baileyi, as measured by Morphomat 10 attached to electron microscope were as follews. Trophozoites' size with range of 3.21+_0.70*2.49+_0.59 micro meter, area with range of 118. 82+_41.92 micro meter2; meronts' size with 3.99+_1.07*2.96+_0.52 micro meter, area 210.11+_57.11 micro meter2 ; merozoites' size 1.98+_0.43*0.60+_0.18 micro meter, area 24.10+_5.97 micro meter2; microgametes' size 1.36+_0.83*0.50+_0.23 micro meter, area 20.23+_6.73 micro meter2; macrogametes'size 4.57+_0.65*4.02+_0.55 micro meter, area 258.37+_51.83 micro meter2; oocytes' size 4.39+_0.56*3.44+_0.50 micro meter, area 187.21 +_62.68 micro meter2. In conclusion, the size and area on each developmental stages of Cryptosporidium baileyi is different from that of other Cryptosporidia spp. It suggests, with considering tissue tropism and life cycle, morphometrical analysis can be quite a good way to identify C baileyi.