Objective: The purpose of this study was to detect the incidence of multi-drug resistant (MDR) and the spread of tet genes that encode tetracycline (TE) resistance in E. coli in pig farms in the city of Kupang, Indonesia.Materials and Methods: Samples of pig feces have been obtained from 96 pig farms in Kupang city, Indonesia. Escherichia coli bacteria were isolated and identified morphologically and biochemically, and finally confirmed by the API test. The disk diffusion method has been used to observe the antibiotic sensitivity effects and has been followed by observing resistant genes encoding TE resistance using the multiplex polymerase chain reaction (m-PCR) method to detect the presence of tet genes such as tet (A), tet (B), tet (C), tet (D), and tet (E), respectively. Results: A total of 82 (85.4%) of E. coli isolates have been found in all pig feces samples obtained from 96 pig farms in Kupang city. This study has shown a high level of antibiotic resistance dominated by erythromycin (85.4%) and cephalothin (58.5%) and followed by several other antibiotics with a percentage below 34.1%. The prevalence of MDR E. coli was 57.3% by showing 39 different patterns. The most common pattern was showed by the Cephalothin-Colistin-Erythromycin pattern. The resistance of E. coli to TE appears to be related to the presence of tet (A) and tet (E) genes.Conclusion: This study has encouraged the need for public awareness (farmers) of the wise use of antibiotics in preventing the spread of resistant bacteria that can cause health problems in animals and humans. [J Adv Vet Anim Res 2018; 5(4.000): 388-396]

Objective: The study was carried out to detect the carriage of methicillin-resistant Staphylococcus aureus (MRSA) and their antimicrobial susceptibilities in village chickens sold at Maiduguri poultry markets using phenotypic characterization. Materials and Methods: This was a cross-sectional study where 120 samples comprised 50% each of Nasal and cloacal swabs, were, respectively, collected from live village chickens sold at Maiduguri poultry markets and examined for the presence MRSA based on bacterial culture, biochemical characteristics, growth on oxacillin resistant screening agar base, and antimicrobial susceptibility tests. Results: The study revealed an overall occurrence of 38.33% S. aureus and 32.60% MRSA, respectively. Antimicrobial susceptibility test was carried out on MRSA positive isolates against seven antimicrobials. All the isolates (100%) exhibited resistance against cefoxitin, whereas the least antimicrobial resistance was recorded against erythromycin and streptomycin each with 26.6%, respectively. In the same way, the highest antibiotic susceptibility in this study was observed against erythromycin (60%) and least susceptibility was against vancomycin and streptomycin with 20% each. A varying intermediate antibiotic susceptibility ranging from 13.33% to 53.33% was observed. Multiple-drug resistance patterns were exhibited by MRSA isolates from this study with 73.3% of the isolates exhibiting resistance to two or more antibiotics. Conclusion: This study has shown the carriage of MRSA by village chickens which calls for serious public health concern and concludes that these birds might have acquired these pathogens from the environment or infected humans since they normally receive no less medical attention. [J Adv Vet Anim Res 2019; 6(2.000): 163-167]

Objective: This study aimed to isolate and identify Escherichia coli from broiler samples from Sukabumi, Indonesia. Also, antibiogram studies of the isolated bacteria were carried out consid¬ering the detection of the antibiotic resistance genes. Materials and Methods: Cloaca swabs (n = 45) were collected from broilers in Sukabumi, Indonesia. Isolation and identification of E. coli were carried out according to standard bacterio¬logical techniques and biochemical tests, followed by confirmation of the polymerase chain reac¬tion targeting the uspA gene. Antibiotic sensitivity test, using several antibiotics [tetracycline (TE), oxytetracycline (OT), ampicillin (AMP), gentamicin (CN), nalidixic acid (NA), ciprofloxacin (CIP), enrofloxacin (ENR), chloramphenicol, and erythromycin] was carried out following the Kirby Bauer disk diffusion method. Detection of antibiotic resistance coding genes was carried out by PCR using specific oligonucleotide primers. Statistical analysis was carried out with one-way anal¬ysis of variance. Results: The results showed that 55.6% (25/45) of the samples were associated with the pres¬ence of E. coli. Antibiotic sensitivity test showed that the E. coli isolates were resistant to TE (88%; 22/25), OT (88%; 22/25), AMP (100%; 25/25), CN (64%; 16/25), NA (100%; 22/25), CIP (88%; 22/25), ENR (72%; 18/25), chloramphenicol (0%; 0/25), and erythromycin (92%; 23/25). On the other hand, the antibiotic resistance coding genes were tetA (86.4%; 19/22), blaTEM (100%; 25/25), aac(3)-IV (0%; 0/16), gyrA (100%; 25/25), and ermB (13%; 3/23). It was found that chlor¬amphenicol is markedly different from other antibiotic treatment groups. Conclusion: Escherichia coli was successfully isolated from cloacal swabs of broiler in Sukabumi, Indonesia. The bacteria were resistant to TE, OT, AMP, CN, NA, CIP, ENR, and erythromycin. Chloramphenicol was more sensitive and effective than other antibiotics in inhibiting the growth of E. coli. The antibiotic resistance genes detected were tetA, blaTEM, gyrA, and ermB. [J Adv Vet Anim Res 2021; 8(1.000): 84-90]

Objective: This study was conducted to determine the occurrence of Salmonella in various meat products (beef from cattle, chevon from goats, mutton from sheep and jaziir from camel), by screening the various selling points which includes; meat retailers in abattoir, markets and shops in Maiduguri and its environs. Materials and methods: A total of 120 samples of fresh meat from cattle, sheep, goats and camels sampled from ten meat retailers in abattoir, markets and shops in the Maiduguri metropolis, using simple random sampling technique. All samples were processed and examined according to standard bacteriological protocols. Results: Percentage occurrence of Salmonella species had the highest value of 15 (50.1%) from the market, found in sheep, while the lowest occurrence of Salmonella species was associated with 3(10.0%) in goats sampled from shop meat.. Antibiotic susceptibility pattern of Salmonella species from cattle meat revealed high resistant to Erythromycin (52%). In sheep, the higher percentage of resistance occurred against Ampicillin (33.3%) and less resistant to Amoxicillin (4%) was obtained. The isolates from camel meat recorded 25% resistant against Ampicillin, Gentamycin and 12.5% to Streptomycin. A total of 28.4% of the isolates were resistant to Ampicillin, Gentamycin and 23.1% to Ofloxacin. Conclusion: The study has shown that Salmonella species are present in fresh meat sold in abattoir, retail markets and shops. We recommend strict hygienic measures in places where fresh meat are sold in Maiduguri metropolis, Nigeria to ensure consumers right to have safe food. [J Adv Vet Anim Res 2017; 4(3.000): 227-233]

Objective: The study was conducted to determine the presence of methicillin resistant Staphylococcus aureus (MRSA) in chickens and farm personnel in Sokoto, North-western Nigeria.Materials and method: A total of 160 samples (136 from chickens and 14 from personnel) were collected and screened for the presence of MRSA using cultural characteristics, biochemical tests and latex slide agglutination test for the presence of Penicillin binding protein 2α (PBP2α). Results: MRSA were detected in 8.82% (n=12/136) of chickens, while 14.29% (n=2/14) in farm personnel. All the isolates were positive for PBP2α.Conclusion: This study established for the first time the occurrence of MRSA in both chickens and farm personnel from poultry farms in Sokoto, Nigeria. Thus, the study provided baseline information for further studies on the epidemiology of MRSA. [J Adv Vet Anim Res 2017; 4(3.000): 255-260]

In this study, efficacies of different therapeutic regimens along with 10% zinc-sulfate footbath for the treatment of acute foot rot in adult sheep were evaluated. The research work conducted on the adult sheep (n=104) of both sexes which were presented to the Teaching Veterinary Clinical Complex during April 2013 to May 2014. Foot rot was confirmed based on clinical and physical examinations. The sheep were divided into four equal groups; G-I, G-II, G-III, and G-IV. The animals of the G-I, II and IV were treated with a mixture of amoxicillin and cloxacillin at 15 mg/kg body weight (b.wt.) through intramuscular (IM) route, oxytetracycline at 20 mg/kg b.wt., IM, and enrofloxacin at 5 mg/kg b.wt., IM, respectively. The animals of G-III were treated with gamma benzene hexachloride cream. Along with the above treatments, all four groups were given footbath with 10% zinc-sulfate. Mean recovery time (days) was recorded as lowest in G-II (3.83±0.64) followed by G-I (4.17±0.31), G-IV (4.38±0.0.79) and G-III (5.67±0.98), respectively. The mean±SE values of rectal temperature and ruminal motility that were recorded before and after the treatment showed significant (p<0.05) differences. In conclusion, administration of parenteral antibiotics in combination with footbath was highly effective to treat combination with footbath was highly effective to treat the acute foot rot in sheep.

A crucial first step in preventing the introduction and spread of harmful microorganisms in poultry farms is biosecurity. The objectives of this investigation were to assess biosecurity measures and the prevalence of different E. coli species in commercial chicken farms in Sharkia province, Egypt. Sensitivity of E. coli strains was identified, and their susceptibility to antibiotics and disinfection was evaluated as well. Four farms provided 21 samples each, for a total of 84 samples. Three samples of each were collected for water, feed, litter, cloaca, wall, hand, and foot boots. All the studied farms obtained a "poor biosecurity" grade, meaning that their overall biosecurity score, which is comprised of 40% external and 36.54% internal biosecurity, was less than 50%. Escherichia coli species were found in 79.76% of the samples. It was isolated from water, feed, litter, cloaca, walls, hands, and foot boots with percentages of 91.6; 83.3; 91.6; 100; 58.3; 58.3 and 75%, respectively. Biosecurity level had significant negative correlations with the isolated E. coil species. Escherichia coli O119 was the most common serotype in litter and the cloaca, while E. coli O1 was the most prevalent serotype in walls and hands. Additionally, serotypes for E. coli O26, O159, O128 and O78 were included. According to the patterns of antibiotic sensitivity, amikacin, nitrofurantoin, gentamycin, and levofloxacin showed intermediate sensitivity, whereas E. coli O119 bacteria were highly sensitive to ciprofloxacin. In the absence and presence of organic matter, Virocid, Cid 2000 and finally Virkon S were the most efficient against E. coli O119. According to this study, there were differences in the investigated farms' biosecurity levels in relation to the occurrence of Escherichia coli. To reduce the introduction and persistence of E. coli in poultry farms, several biosecurity practices and management, including stringent cleaning and disinfection measures should be applied.

Mycoplasma synoviae (MS) and Mycoplasma gallisepticum (MG) are two of the most serious infections in poultry, causing financial losses across the globe. Antibiotics treatment can lessen the clinical signs of MG and MS infection in birds and help restrict the spread of these infections. However, the rise of antibiotics multidrug resistance (MDR) among poultry diseases is still a problem for the world's health. Minimum inhibitory concentration (MIC) is crucial to resolving this issue since it helps ensure correct antibiotic use and fights the development of antibiotic resistance. This investigation aims to assess the in vitro and in vivo efficacy of several antibiotics (tilmicosin, tylosin, erythromycin, spiramycin, lincomycin, and doxycycline) against MG and MS field isolates, in addition to guava leaves extract (GLE) against MG. For in vivo study, 510, one day old chicks were randomly split into 17 groups the birds were experimentally infected at 3 days old and supplied with different treatments in drinking waters for 5 successive days at 18 days old. The results of in vitro study showed that tilmicosin was the most effective against MG isolates with MIC values ranging from 0.0078 to 0.0156 μg/ml and spiramycin considered effective against MS with MIC values ranging from 0.015625 to16μg/ml and MIC value of GLE was 0.25 μg/ml that was considered as moderate sensitive to MG infection. The in vivo study revealed that tilmicosin and spiramycin were able to significantly lower the clinical score, lesion score, and re-isolation rate of MG and MS, respectively in the treated birds at p-value < 0.05 in contrast with control positive infected untreated groups. Additionally, the treated groups with tilmicosin in MG infection and spiramycin in MS infection showed significantly higher mean body weights (MBW) compared to the infected untreated ones at p-value < 0.05. The current study demonstrates that MG and MS's sensitivity to many of the most popular drugs changed as in tylosin become low sensitive and erythromycin become resistant. GLE has moderate sensitivity antibacterial effect against MG isolates both in vivo and in vitro and can be used as a natural antibiotic alternative. Therefore, we recommend periodical monitoring of the sensitivity of the circulation MG and MS strains using MIC test to overcome MDR of avian mycoplasmas with further suggestion to evaluate GLE as nano-preparation against MG infection.

Objective: In the context of the fast-expanding shrimp farming industry in Sri Lanka, this study aimed to evaluate the distribution and understanding of BMPs, examine the relationship between BMPs and disease outbreaks, and analyze the current antibiotic usage trends through a fact-finding survey. Materials and Methods: A questionnaire survey was conducted in 131 shrimp farms located in Puttalam District in North Western Province, where shrimp farming is thriving in Sri Lanka. The survey was conducted from September to October 2021. In addition, in August 2022 and August 2023, interviews were conducted with shrimp farms in Puttalam district and Batticaloa district, shrimp hatcheries, and export companies. Data were analyzed using descriptive statistics, Probit, and Tobit regression analysis. Results: The item-count technique revealed a significant (p &lt; 0.05) difference in the use of antibiotics without consulting experts, signifying inappropriate use. No aquaculture farmer reported being unaware of BMPs, and among the 45 farmers who attended a BMPs seminar, 30 claimed to possess a good understanding of BMPs. Probit and Tobit regression results revealed that the rate of understanding of BMPs, education level, and obtaining information on the sanitation management of shrimp farming from extension and guidance organizations were inversely associated with both disease incidence in shrimp farming and shrimp discards. Conclusion: Although the use of antibiotics is prohibited in BMPs, the analysis results suggest inappropriate use of antibiotics. The findings indicate that enhanced BMP understanding can reduce disease incidence and shrimp discards, emphasizing the need for incentives to promote BMP adoption and reduce the necessity for antibiotics. [J Adv Vet Anim Res 2024; 11(1.000): 33-39]