The researcher’s attention nephrotoxicity from antibiotics (as aminoglycosides), non-steroidal anti-inflammatory drugs, and antifungals, angiotensin-converting enzyme (ACE) inhibitors. Several drugs have resulted in produce an adverse effect on kidneys. Angiotensin-converting enzyme (ACE) is a component of the renin-angiotensin system (RAS), which leads to the conversion of Angiotensin-I to Angiotensin-II in vascular tissues. The aim of this work was to investigate the effect on serum angiotensin converting enzyme of the amikacin. In this study, two different groups were formed as control (10 rats / Wistar-albino female) and experimental group (30 rats / Wistar-albino female). The experimental group was administered 15 mg/kg amikacin intraperitoneally (ip) for 14 days, and the control group was administered saline solution at the same rate.When the groups are compared according to the statistical results, it is seen that there is a significant increase in ACE activity of the experimental group compared to the control group (p˂0.001).As a result, it was determined that amikacin administered increased serum ACE activity and it was concluded that it may be useful to investigate the possibilities to evaluate it as a risk factor and indicator in the development of hypertension.