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Studies on staphylococci isolated from bovine mastitis, 3; activity of penicillins and gentamicin to isolates
1984
Park, C.K. (Kyongbuk National Univ., Taegu (Korea R.). Dept. of Veterinary Medicine)
The in vitro activity of penicillins (penicillin G, ampicillin, carbenicillin, methicillin and cloxacillin) and gentamicin to Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitic milk samples was determined. The growth of all isolates of staphylococci tested was inhibited by cloxacillin and methicillin at concentration of 0.78 micro g/ml and by gentamicin at a minimum inhibitory concentration (MIC) of 1.56 micro g/ml
Mostrar más [+] Menos [-]Studies on the factors influencing the transformation in Escherichia coli with pBR 322 DNA
1984
Yoo, H.S. | Mah, J.S. (Seoul National Univ., Suwon (Korea R.). Coll of Veterinary Medicine)
To investigate the factors influencing the artificial transformation in Escherichia coli, E. coli C 600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCL-EtBr equilibrium density gradient centrifugation from E. coli HB 101. The best conditions for the transformation of E. coli C 600 with pBR322 DNA were treatment with 100mM CaCl2, ice incubation at 0 deg C for 45 minutes, heat shock at 42 deg C for 4 min., 30 min. of ice incubation and incubation at 37 deg C for 100 min. for gene expression in that order
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