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A monoclonal antibody, 169.1, against canine leukocyte surface antigen identifies cytoskeletal components in epithelial cells and peripheral neurons
1997
Iwami, Y. (Hokkaido Univ., Sapporo (Japan)) | Hashimoto, Y. | Iwanaga, T.
Effect of neutralizing monoclonal antibodies on Hantaan virus infection of the macrophage P388D1 cell line
1992
Yao, J.S. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Arikawa, J. | Kariwa, H. | Yoshimatsu, K. | Takashima, I. | Hashimoto, N.
Antigenic analysis of Japanese encephalitis virus isolated in Hokkaido with monoclonal antibodies
1989
Ochiai, K. (Yokohama Univ. (Japan). Faculty of Engineering) | Takahashi, I. | Hashimoto, N.
Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus
2008
Kariwa, H.(Hokkaido Univ., Sapporo (Japan)) | Noda, H. | Nakauchi, M. | Ishizuka, M. | Hashiguchi, K. | Hashimoto, S. | Yoshii, K. | Asano, A. | Agui, T. | Kogaki, H. | Kurano, Y. | Uchida, Y. | Fujii, N. | Okada, M. | Takashima, I.
The sudden emergence of severe acute respiratory syndrome (SARS) at the end of 2002 resulted in 774 reported deaths from more than 8000 cases worldwide. As no effective vaccines or antiviral agents are available, the most effective measure to prevent the expansion of a SARS epidemic is the rapid diagnosis and isolation of SARS patients. To establish specific diagnostic methods, we generated nine clones of monoclonal antibodies to nucleocapsid protein (NP) of SARS-coronavirus (SARS-CoV). On immunofluorescent antibody assay and Western blotting analysis, none of the monoclonal antibodies showed cross-reactivity to authentic and recombinant NPs of human coronavirus (HCoV) 229E strain. To determine the region on the NP molecule where the monoclonal antibodies bind, we generated four truncated recombinant NPs and analyzed the reactivity between monoclonal antibodies and truncated NPs. Two monoclonal antibodies reacted with a truncated NP covering from amino acid residues 111 to 230, and seven reacted with another truncated NP covering from amino acid residues 221 to 340. Epitope mapping analysis indicated that monoclonal antibody SN5-25 recognized the amino acid sequence Qsup(245)TVTKKsup(250) on SARS-NP. Within the epitope, Q245, T246, V247, K249, and K250 appeared to form an essential motif for monoclonal antibody SN5-25 to bind. The information about binding sites and epitopes of monoclonal antibodies may be useful for the development of new diagnostic methods for SARS and for analyzing the function of N protein of SARS-CoV.
Mostrar más [+] Menos [-]Characterization of two monoclonal antibodies which recognize different subpopulations of chicken T lymphocytes
1990
Kondo, T. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Hattori, M. | Kodama, H. | Onuma, M. | Mikami, T.