Therapeutic and anti-oxidant potential of vitamin E and selenium was evaluated in mastitic cows. The CMT point score and milk somatic cell count (SCC) was significantly (P0.05) higher in mastitic cows prior to treatment. Reduced activity of enzymatic anti-oxidant catalase was observed in mastitic animals. The parameters studied in mastitic cows treated amoxicillin sulbactum (group II) and with amoxicillin sulbactum, vitamin E and selenium (group III) were compared with that of group I healthy cows. Significant reduction (P0.05) in CMT and SCC was observed in both the treated groups. However, reduction in CMT point score and SCC was more pronounced in group III than group II cows. Synergistic action of antibiotic and vitamin E selenium significantly (P0.05) enhanced the activity of catalase in group III mastitic animals on day 8 time period. Therefore, vitamin E and selenium supplementation could be advised in the therapy of mastitis.

Oxidative stress is a common mechanism contributing to initiation and progression of hepatic damage in a variety of liver disorders. Hence, there is a great demand for the development of agents with potent antioxidant effect. The aim of the present investigation is to evaluate the efficacy of raw and nanoforms of resveratrol and silibinin as a hepatoprotective and an antioxidant against diethylnitrosamine induced hepatocellular damage. 0.01% diethylnitrosamine administration to rats resulted in significantly elevated levels of lipid peroxidation (LPO) and decreased levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GSH) and total protein (TP) in the liver tissue. Post-treatment with the nano resveratrol (50 mg/kg), nano silibinin (50 mg/kg), raw resveratrol (50 mg/kg), raw silibinin (50 mg/kg) orally for 30 days significantly reversed the diethylnitrosamine induced alterations in the liver tissue and offered almost complete protection. The results from the present study indicate that both nano forms exhibit good hepatoprotective and antioxidant potential against diethylnitrosamine induced hepatocellular damage in rats as compared to raw forms.