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Biological control of Flavobacterium psychrophilum infection in ayu (Plecoglossus altivelis altivelis) using a bacteriophage PFpW-3
2018
Kim, J.H., Seoul National University, Seoul, Republic of Korea | Park, S.C., Seoul National University, Seoul, Republic of Korea
The efficacy of using a bacteriophage (phage) to control Flavobacterium psychrophilum (F. psychrophilum) infection of ayu (Plecoglossus altivelis altivelis) was evaluated in this study. Intramuscular challenge failed to induce sufficient infection levels; therefore, a newly designed net-scratch challenge method was also used to induce bacterial infection. Administration of phage PFpW-3 in F. psychrophilum-infected ayu showed notable protective effects, increased survival rates and mean times to death. Additionally, the fate of inoculated bacteria and phage in ayu were investigated. Our results suggest that the phage PFpW-3 could be considered an alternative biocontrol agent against F. psychrophilum infections in ayu culture.
Mostrar más [+] Menos [-]Evaluation of in vitro and in vivo bacteriophage efficacy against Salmonella enterica serovar Enteritidis infection
2010
Cha, S.B., Seoul National University, Seoul, Republic of Korea | Rayamajhi, Nabin, Seoul National University, Seoul, Republic of Korea | Lee, W.J., Seoul National University, Seoul, Republic of Korea | Shin, M.K., Seoul National University, Seoul, Republic of Korea | Roh, Y.M., Seoul National University, Seoul, Republic of Korea | Jung, M.H., Seoul National University, Seoul, Republic of Korea | Myoung, K.S., RnD center, Korea Yakult, Yongin, Republic of Korea | Ahn, Y.T., RnD center, Korea Yakult, Yongin, Republic of Korea | Huh, C.S., RnD center, Korea Yakult, Yongin, Republic of Korea | Yoo, H.S., Seoul National University, Seoul, Republic of Korea
Salmonella (S.) Enterica infection ranks among the most common food borne bacterial infections worldwide. Although there are six subspecies of S. Enterica, the vast majority of human and animal infections are caused by strains belonging to subspecies 1 serovar Typhimurium and Enteritidis. Recent reports on antibiotic resistance of Salmonella spp. are rising steadily. The increasing problem of antibiotic resistance has rekindled interest in bacteriophage to therapy. Therefore, we investigated the efficacy of bacteriophage in S. enterica serovar Enteritidis infected mice and pigs by measuring of body condition, body weight, bacterial colonization and weight of organs based on the in vitro analysis. In vitro experiment, phage cultured with S. Enteritidis showed clear lysis pattern, the plaque forming unit (PFU) of our phage culture was 1.5 × 10∨11 PFU/mL, and phage showed its maximum activity at 4 h post inoculation. In mouse experiment, there was no significant difference among experimental groups in the general body conditions and body weight of mice. However, there was difference in weight of liver and spleen depending on the experimental group (p less than 0.05). The weight of liver and spleen were reduced by the phage treatment. Also bacterial colonization in spleen and liver were significantly reduced by the phage treatment. In pig experiment, the general body conditions and body temperature exhibited not much difference among the pigs except few pigs in group 3 which showed poor body conditions. From the feces in each group, we could isolate the S. Enteritidis only from group 3. Bacterial enrichment culture was necessary for isolating the bacteria from 5 dpi and 10 dpi, however direct isolation was possible from 15 dpi feces. In phage treated group, postmortem lesion was better than non-phage treated group. Recently, antibiotic resistance concerns on the food-borne bacterial pathogens have been increasing because of the wide spread of the antibiotics resistance genes. This concern is widely transmitted to the human related public health. As one of the alternative treatments on the bacterial pathogens, attempt using phages have been made to control the bacterial diseases. The positive possibility of the trail using phage was observed to control the S. enterica serovar Enteritidis in this study even though the further analysis has been remained.
Mostrar más [+] Menos [-]Field evaluation of Enterotoxigenic Escherichia coli-specific bacteriophage (ΦCJ19) as a feed additive
2013
Yoo, A., Seoul National University, Seoul, Republic of Korea | Cha, S.B., Seoul National University, Seoul, Republic of Korea | Shin, M.K., Seoul National University, Seoul, Republic of Korea | Park, H.T., Seoul National University, Seoul, Republic of Korea | Seo, H.S., CJ Cheil Jedang Co., Seoul, Republic of Korea | Kim, J.W., CJ Cheil Jedang Co., Seoul, Republic of Korea | Yoo, H.S., Seoul National University, Seoul, Republic of Korea
Field efficacy of enterotoxigenic Escherichia coli-specific phage (ΦCJ19) as a feed additive was evaluated in weaning piglets. Fifty-four piglets at 3~4 weeks old were allocated in three different groups and two of them were fed with bacteriophage at different concentrations (106 PFU/kg feed and 108 PFU/kg feed, respectively) for 30 days. Body weight and feed intake were measured at 10 days interval and body condition and fecal score were inspected every day. Based on the measurement, feed conversion rate (FCR) and average daily gain (ADG) of each group during 30 days were analyzed. The analysis suggests that the bacteriophage may help the improvement of FCR and ADG at 108 PFU/kg of bacteriophage feeding group in 30 days. A result from analysis of fecal score indicates that the bacteriophage also may help to relieve the intermittent diarrhea in post-weaning stage. Those results suggest that bacteriophage might help the growth of piglets in post-weaning stage.
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