When Salmonella typhimurium and Clostridium perfringens were tested in conventional chickens, larger numbers of S typhimurium and C perfringens adhered to Eimeria tenella-infected ceca than to uninfected ceca. In germ-free chickens, S typhimurium and C perfringens adhered to the E tenella-infected cecal mucosa more than to the uninfected cecal mucosa, but fewer Bacteroides vulgatus and Bifidobacterium thermophilum adhered to the E tenella-infected ceca than to the uninfected ceca. Many bacteria adhered to the lesions caused by E tenella as observed by scanning electron microscopy. On the basis of our findings, we suggest that infection with E tenella upsets the balance of competitive adherence of bacteria, allowing more colonization of S typhimurium and C perfringens.

Studying of therapeutic effectiveness of metabolic probiotic Diamixan (sterile concentrate of waste products of Lactobacillus acidophilus, Lactobacillus debrueckii, and Streptococcus salivarius) against piglet gastroenteritis appearing at weaning period was realized in the conditions of the Republic of Belarus. The analyzed preparation contained complex of substances: bioartificial lactic acid, amino acids, polysaccharides, and some other ingdredients. In course of analysis of the state of intestinal microbiocenosis there were analyzed the following indexes: bifidus bacteria; lactic acid bacillus; E. coli; enterococcus; Proteus; Staphylococcus saprophyticus; Clostridia; yeast-like fungi; enterobacteria. Research results showed that application of the examined preparation made it possible to shorter the terms of animal recovery on 2,2 days, as well as to facilitate the severity of a disease. Bottle feeding of the metabolic probiotic promoted the stabilization and normalization of the main hematological indexes. The analyzed preparation created the optimal pH in lumen of the intestine, inhibited the rowth of opportunistic pathogenic bacteria, promoted quicker reconstruction of microecological status by means of normalization of standard flora of bifidus bacteria and lactic acid bacillus

There was studied the influence of parasites on quantitative and qualitative characteristics of gastrointestinal microflora of animals. The digestive path represents open system by means of which contact of macroorganism to environment and present at it microbes is carried out. For normal functioning of digestive system the essential role is played by its microbiocenosis condition. During the research some regularities in the rumen and intestine microflora content under the influence of parasites (e.g. N. vitulorum and in assotiation with Eimeria spp. and Strongylata spp) have been postulated. They are expressed by quantity reduction of bifido- and lactoflora with simultaneous increase of content of conditional-pathogenic intestinal stick, aerobic bacillus and micromicet; decrease of quantitative and qualitative structure of protozoa rumen, decrease in their mobility, and also decrease in activity of cicatricial microflora. | Целью работы является изучение влияния паразитов на количественную и качественную характеристики микрофлоры желудочно-кишечного тракта животных. Пищеварительный тракт представляет собой открытую систему, посредством которой осуществляется контакт макроорганизма с внешней средой и присутствующими в нем микробами. Для нормального функционирования пищеварительной системы существенную роль играет состояние ее микробиоценоза. В ходе исследований установлены определенные закономерности в изменении микробиоценоза рубца и кишечника под влиянием паразитарных агентов у телят (а именно, при паразитировании Neoascaris vitulorum в виде моноинвазиии или в ассоциации с Eimeria spp. и Strongylata spp.), которые выражаются уменьшением количества бифидо- и лактофлоры с одновременным увеличением содержания условно-патогенной кишечной палочки, аэробных бацилл и микромицет; нарушением количественного и качественного состава простейших рубца, снижением их подвижности, а также снижением активности рубцовой микрофлоры.

Development of diarrheal diseases of newborn animals has a miltifactorial character and it is very difficult to optimize the structure of microbial population in alimentary tract and to carry out the correction of the microbe status only by means medical preparations. Therefore, for regulation of standard structure of intestine microflora in a complex of medical and preventive measures for diarrhea diseases of young poultry an important role is played by application of probiotics, prebiotics, and immunoamplifiers. In course of study there were used biologically active preparations produced on the basis of ecologically pure products of a natural origin: immunostimulant Alveozan, probiotics Bifidoflorin liquid and Dialakt, and prebiotics Biofon АIL and Biofon. Research was realized on one-day-old broiler chickens of Kobb-500 cross. The obtained data on the positive influence of the studied preparations on microbiocenosis of an alimentary tract of broilers chickens correlated with the indexes of natural resistance (autarcesis).The analyzed preparations made it possible to increase the local protection, stimulation of hematopoiesis (increasing of hematoglobulin, erythrocytes and leukocytes); to increase the blood content of whole protein, albumens and globulins, bactericidal activity of blood serum. Complex introduction of the analysed preparations stimulated the natural disease resistance factors, normalized the metabolic function of poultry, promoted the increasing of chicken livability and productivity

There was studied the prebiotic (Biofon AIL) and probiotic (Bifidoflorin liquid) preparations on a condition of bacterial flora of chickens-broiler intestines in age dynamics and also to estimate efficiency of joint application of prebiotics and probiotics. Biofon AIL represents mannan-oligosaccharides which are capable to block colonisation of intestines by pathogenic microflora and possess stimulating action on growth of bifidus bacteria in culture. Bifidoflorin liquid represents liquid microbic mass of bifidus bacteria. As a result of the spent research it is revealed, that inclusion of prebiotics Biofon AIL and probiotics Bifidoflorin liquid to a ration of chickens-broilers allows to spend correction of bacterial cenosis intestines towards prevalence of bifidus bacteria and lacto bacteria. Thus the best indicators are noted at sharing of these preparations. In the tabular form the data on dynamics bacterial cenosis intestines at chickens-broilers are presented at use of prebiotics and probiotics. | Целью работы явилось изучение пребиотического (Биофон АИЛ) и пробиотического (Бифидофлорин жидкий) препаратов на состояние бактериальной флоры кишечника цыплят-бройлеров в возрастной динамике, а также оценить эффективность совместного применения пребиотика и пробиотика. Биофон АИЛ представляет собой маннанолигосахариды, которые способны блокировать колонизацию кишечника патогенной микрофлорой и обладают стимулирующим действием на рост бифидобактерии в культуре. Бифидофлорин жидкий представляет собой жидкую микробную массу бифидобактерии. В результате проведенных исследований выявлено, что включение в рацион цыплят-бройлеров пребиотика Биофон АИЛ и пробиотика Бифидофлорин жидкий позволяет провести коррекцию бактериоценоза кишечника в сторону преобладания бифидобактерий и лактобактерии. При этом лучшие показатели отмечены при совместном использовании этих препаратов. В табличной форме представлены данные по динамике бактериоценоза кишечника у цыплят-бройлеров при использовании пребиотиков и пробиотиков.