A comprehensive study was conducted on the clinical observationsincluding clinical history, physical examination along with haematobiochemical alteration on 41 naturally occurring cases of canine babesiosis from Punjab state, India. Examination of 964 dogs revealed 4.25 percent (41/964) prevalence of the disease including 3.84 percent (37) B. gibsoni and 0.41 percent (4) B. canis infected cases. Clinical and parasitological diagnosis was finally confirmed by polymerase chain reaction. A large variation of clinical anifestations including rare findings of paraplegia, blindness, ocular bleeding, immune mediated haemolytic anaemia (IMHA), ascites and skin lesions were observed among the affected animals. Bloodfilms showed anisocytosis and nucleated erythrocytes indicating regenerative anaemia. Blood parameters of the affected dogs revealed significant decrease in Hb, TEC, PCV and thrombocytes. Significantdecrease in lymphocytes was found in B. gibsoni affected animals. The affected dogs showed significant increase in serum bilirubin, ALT, AKP, BUN and creatinine. Haemato-biochemical observations wereindicative of severity of babesiosis in dogs.

The study was conducted in 18 clinically healthy male dogs of about 1 year of age, weighing up to 12 kg and divided into three groups of 6 animals each. Bupivacaine alone, bupivacaine + hyaluronidase and hyperbaric bupivacaine were given epidurally at lumbosacral space @2 mg/kg. bwt. with a maximum dose of 3 ml in the animals of groups I, II and III, respectively. A significant increase (P0.01) in rectal temperature, heart rate and respiration rate was recorded at initial intervals of observation in all the groups and thereafter these values follow a declining trend in all groups consistently and reached to almost base line value at 120 min. of observation The alterations of glucose, serum urea nitrogen, aspartate aminotransferase and creatinine estimated at 1hr, 2hrs, 4hrs and 24 hrs were remained within physiological limits in all the groups.Therefore induction of regional anaesthesia with bupivacaine and its combination revealed no permanent alteration in the biochemical and physiological parameters hence it is safe and satisfactory anaesthesia.

The canines affected with skin affections were subjected to blood biochemical profile assay. The affections included dermatitis, sarcoptic mange, demodectic mange, eczema, flea allergy dermatitis, skin allergy and bacterial dermatitis. The blood profile of serum iron, triglyceride, cholesterol, uric acid, urea, creatinine, lactate dehydrogenase, and gamma glutamyl transpeptidase were compared with the blood of healthy dogs. The serum iron, urea and creatinine were comparable in both the groups. It was concluded that stress of dermatosis reflected in enzyme (higher in GGT) and lipid profile (higher in triglyceride, cholesterol) of patients, while renal parameters (urea and creatinine) remain unaffected on account of skin ailments.

It was established that in blood serum of pigs vaccinated against leptospirosis there was an authentic increasing of alanine aminotransferase, alkaline phosphatase and gamma-glutamyltransferase that made proved the presence of residual reactogenicity of biological preparations. In course of the experiment there were used 4 groups of swine which were administrated with 4 types of vaccines: 1. polyvalent vaccine produced in Belarus; 2. experimental thiosulfate vaccine in which there was used 30% sodium solution; 3. experimental emulsified vaccine; experimental emulsified + sodium + thiosulfate vaccine.

The research directed on perfection of a cattle protein food system, taking into account its fractional structure, and also establishment of objective indicators of an estimation of forage protein nutrient density, have great value. It is established, that for young growth of cattle of 4-6 months of age the optimum parity of decomposable and un-decomposable protein in rations corresponds to size 68:32, allowed to raise digestibility of nutrients on 3-4%, nitrogen adjournment in an organism of animals on 10% and daily average a gain on 4% in comparison with parity 80:20. The optimum parity of fractional structure of protein makes positive impact on oxidation-reduction processes in organism to what testifies morphological-biochemical structure of blood. Thus concentration of crude protein raises on 1,7%, urea quantity decreases on 23%. The least cost price of a gain has appeared in group with a parity of decomposable and un-decomposable protein in rations 68:32, or it is better on 4,4% than an indicator of group with a parity 80:20%

In the conditions of the Republic of Belarus there was studied the clinical and biochemical status of pregnant cows, as well as the determination of the main reasons of disease incidence of new-born calves with dyspepsia (dyspeptic disorder). Realized clinical and biochemical studies showed that the inappropriate feeding of pregnant cows in animal breeding farms in housing season of husbandry caused the disorders of metabolic processes in the experimental animals. In blood there was noted the lowering of indexes of alkalinity reserve, glucose, phosphorous, carotine, disturbance of calcareous and phosphorous ratio. In other words, in pregnant cows there were stated the latent forms of osteodystrophia, hypovitaminosis A, hypoglycemia, and acidosis. All these factors caused the irritation and damage of liver and other organs. Mammary gland of diseased animals released colostrum milk of low quality and according to the content of nutritious and protective elements quantity it was of low quantity. Besides, derangements in calf feeding of pregnant nonmilking cows led to birth of calves with low resistance indexes to the influence of external environment factors.

This article is devoted to a problem of biochemical interaction of selenium and antioxidational system of animals and people organism. The problem we have touched is about the efficiency of influence of organic and inorganic taking place on subcellular level. The advantages of organic selenium are proved, the degree of its bjochemical influence on an antioxidationi status of health are indicated.

In the conditions of the Republic of Belarus after fasciolosis invasion of cattle for the biochemical diagnostics of liver pathologies there was composed a hepatospecific metabolic profile of blood serum of cattle into which there were included several enzymes (alanine aminotransferase; aspartate aminotransferase; gamma-glutamyl transpeptidase; alkaline phosphatase; and cholinesterase), content of total albumin, serum albumin, total cholesterin and total bilirubin, as well as colloid tests (thymol turbidity test and test for apoliproteins). There was revealed a intrahepatic cholestasis developed during fasciolosis infestation, as well as dysproteinemia as a compensatory mechanism of liver proteinsyntetic function.