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Sustained hypomyelination and high serum thyroid hormone in aged black tremor hamster
2003
Kim, H.O. (Hokkaido Univ., Sapporo (Japan)) | Kimura, T. | Ochiai, K. | Yazawa, H. | Itakura, C. | Umemura, T.
Oligodendrocytes and myelin in the corpus callosum of black tremor and normal hamsters aged over 1.5 years were ultrastructurally examined to determine the myelination index (ratio of myelin thickness/diameter of axon), percentage of naked axons, and proportions of oligodendroglial subtypes (light, medium and dark). The mutant hamsters were remarkably hypomyelinated, with a low myelination index and a high proportion of naked axons, and high proportions of the dark subtypes. Serum concentrations of thyroid hormones (T sub(3) and T sub(4)) in 6-week-old mutant hamsters were 2-fold (T sub(3)) to 3-fold (T sub(4)) higher than those of age-matched normal animals. However, in the aged animals (over 1.5 years old) only T sub(4) levels of the mutant hamsters were higher in the mutant than normal hamsters. The black tremor hamsters were hypomyelinated throughout their life and high serum level of thyroid hormones might have played a role in the hypomyelination.
Mostrar más [+] Menos [-]The canine alkaline phosphatases: A review of the isoenzymes in serum, analytical methods and their diagnostic application
1998
Syakalima, M. (Hokkaido Univ., Sapporo (Japan)) | Takiguchi, M. | Yasuda, J. | Hashimoto, A.
This paper reviews the alkaline phosphatases in canine serum, the analytical methods used for qualitative and/or quantitative detection of these isoenzymes, and the diagnostic significancy of each of these isoenzymes. The paper further describes some of the latest advances of our knowledge of the canine alkaline phosphatases and possible areas of future research
Mostrar más [+] Menos [-]Evaluation of a new enzyme-linked immunosorbent assay to detect keratan sulfate in equine serum
2010
Lettry, V., Hokkaido Univ., Sapporo (Japan) | Kawasaki, H. | Sugaya, K. | Hosoya, K. | Takagi, S. | Okumura, M.
This study aimed to evaluate a system that identifies cartilage turn over and/or degradation through measurement of a new keratan sulfate (KS) epitope concentration in equine sera. Blood samples were collected from 30 horses, 1 (n=15) and 2 year-olds (n=15). Serum samples were analyzed for an epitope of keratan sulfate by 1/20/5D4 (KS5D4) and new epitopes of keratan sulfate using high sensitive keratan sulfate (HSKS), measured by two respective enzyme-linked immunosorbant assays (ELISAs). There was no correlation in serum concentration of KS evaluated using 5D4 and HSKS. Age had no significant effect on concentrations of KS measured with KS5D4 while 1 year-old horses showed significantly higher amounts than 2 year-olds with HSKS. Results suggest that HSKS could detect early signs of cartilage metabolic changes.
Mostrar más [+] Menos [-]N-terminal amino acid sequence of a 28 kDa major serum high density lipoprotein of the rainbow trout Oncorhynchus mykiss
1994
Murata, M. (National Inst. of Health, Tokyo (Japan)) | Sugimoto, C. | Kodama, H. | Onuma, M.
Reference data on the anatomy and serum biochemistry of the silver fox
1991
Zhan, Y. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Yasuda, J. | Too, K.
Use of biotinylated antibody for the assay of Hanganutziu-Deicher antibodies and antigens in fluids and tissues from cancer patients
1989
Gathuru, J.K. (Yokohama Univ. (Japan). Faculty of Engineering) | Higashi, H. | Kato, S. | Usuba, O. | Naiki, M.