Globally, genetically modified (GM) crops were grown on 191.7 million hectares in 2018, which were mostly sown with soybean, maize, cotton, oilseed rape, and rice. The most popular traits introduced through genetic modification include herbicide and pest insect resistance. The aim of this study was to identify and quantify genetically modified soybean used in animal feed in Poland. This research was based on the real-time PCR technique. All methods for GM soybean events were adopted from the EURL GMFF database of methods and previously verified to meet the minimum criteria of acceptance. Over 15 years of research, 665 samples were examined in total. The most common GM soybean event was MON40-3-2, tested for from the beginning of the investigation. Next, in decreasing order of frequency, were MON89788, MON87701, and A2704-12. In the majority of samples (606; 91%) GM soybeans were identified at a content level above the 0.9% GM content threshold for mandatory labelling. Only 59 soybean samples (9%) were identified as GM negative. GM negative results were mainly identified during the analyses in the last three years of the study, from 2017 to 2019. Our data clearly indicate that the majority of soybean used in Poland for animal feeding was genetically modified.

In course of realized in the conditions of the Republic of Belarus studies there was analysed the chemical composition and nutrient value rape (Brassica napus) phospholipids. Research results proved that rape phospholipids contain in their composition the required quantity of energy nutrient substances (extractive substances, protein, carbohybrates) with moisture content mass - 5,1%, dry solids weight ratio - 94,9%, crude ash weight ration - 5,7%, weight fraction of fat and extractive substances - 35,5%, weight fraction of dry ethanol extract - 50,0%, weight fraiction of crude protein - 28,2%, weight fraction of crude fiber - 6,4%, totals umber of carbonhydrates including easily hydrolized - 24,4%, carotene content - 21,50 mg/kg, iron – 225,5 mg/kg, manganese – 10,4 mg/kg

Study of the regeneration processes and raising the efficiency of rape (Brassica napus) organs and tissues cultivation in vitro methods, as well as the achievement of a stable yield of proper regenerants were realized in the conditions of the Republic of Belarus. The obtained data specified the possibility of generation of rape finished regenerants in vitro culture without additional passages and transfers by means of effectively selected concentrations of phytohormones and biologically active substances, which made it possible to realize principles of totipotency and polarity of crop vegetative organs. On the explant part which was submerged into culture medium there was formed a root, and on opposite one - the morphogen structures. The possibility of obtaining rape regenerants from hypocotyls segments by straight regeneration was studied. Application of the analyzed substances considerably simplified and reduced the price of process of full regenerants formation, and made it possible to receive a wide spectrum of various forms that was important for selection process due to the opportunity to select valuable (in protein content) samples for the further studies and their inclusion into production process

In the conditions of the Republic of Belarus there was determined of a dose of input and efficiency of feeding with rape oil cake and protein meal produced on the basis of a new rape (Brassica napus) variety Yavar as a part of a mixed fodder KR-2 in diets of young stock of cattle. Rape oil cake and protein meal with the content of 1,4-1,9% glucosinolates and 27-30 mcM per 1 kg of a dry matter of erucidic acid can be included into composition of the mixed fodder КR-2 for bull calves in a dose up to 20% on weight. Feeding with the mixed fodders КR-2 with inclusion of rape oil cake and protein meal made it possible to receive daily average weight gain of calves at a level of 827-906 gram at expenses of forages of 5,4-5,9 forage units per 1 kilogram of a weight gain. It was caused by cheaper rape forages. In course of the study there was analyzed the chemical composition of rape oil cake and protein meal; indexes of rumen digestion of experimental bull calves; morphological and biochemical blood structure; changes of body weight; daily average weight gain and expenses for forages.

Feeding replacement heifers of 6-12 months of age with mixed feed that contains 20-25% of rape and lupine grain within winter and summer diets instead of sunflower cake allows to obtain the average daily weight gain of 893-927 g at forage spends of 6,0-6,3 с of forage units. The cost of these mixed feeds is decreased at 14% and prime cost of 1 c of weigh gain-at 6-15%

Elaboration of production technology of rape (Brassica napus) phospholipids as one of components of feed additive was realized in the conditions of the Republic of Belarus. The following equipment was used for rapeseed processing: conveyer screw for seeds, conveyer for pressed seeds (seed cake), press Farraet Zralok LC preliminary, press - Farmet Zralok PL finishing, storage-capacity for oil, storage with mixing mechanism, automatic leaf filter, compressor, air vent, air filter. Rape oil was the main product of rape processing and oil seed press cake and rape phospholipides were the additional products. Quality indexes of rape phospholipids (appearance; quantity of obtained phospholipids; moisture content; dry matter content; oil and extractive matter content; total amount of carbohydrates) were analyzed. Results of phospholipids production depending on period of filtration and pressure load in the process of oil filtration were presented. Results of rape phospholipids production depending on air pressure were given. There were obtained the following optimal parameters for phospholipids production: optimal filtration time of freshly squeezed rape oil – 5-6 hours; optimal pressure load of during the filtration of raw rape oil - 2,5-3,0 atmospheres; and optimal air pressure during the filter drying - 2,5-3,0 atmospheres. The proposed production technology of rape phospholipids made it possible to produce 4,5-5,5% of phospholipids from 1 kg of rape

Rape-cake containing 1,4-1,9% of glycozinolates and 27-30 mMol of erucic acid per 1 kg of dry matter may be implemented in mixed feed KP-1 for calves in amounts of 15% on mass. Feeding animals with mixed feeds KP-1 with rape-cake allows to get the average daily weight gains of 848-865 g at forage spends of 2,49-2,52 forage units per 1 kg of weight gain