BACKGROUND: Nowadays, researchers are converted silver to nanometer dimensions and using nanotechnology to achieve suitable performance and preserve the poultry health and they are used silver nanoparticles are used for poultry nutrition as a nutritional supplement. On the other hand, at the molecular level no complete reports in poultry breeding have been provided. OBJECTIVES: This study was conducted to evaluate cytochrome P450 gene expression in broiler chickens fed with silver nanoparticles. METHODS: This experiment a completely randomized design with 450 single-day Cobb 500 broiler chicks was divided into five treatments and six replications (15 birds in each replicate). Chickens were fed with (1) control (basal diet), (2) basal diet containing 1% zeolite, (3) basal diet containing 1% of zeolite-coated with 0.5% silver nanoparticles, (4) Basal diet containing with 0.15% organic acids and (5) Basal diet containing 1% of zeolite-coated with 0.5% of silver nanoparticles and 0.15% organic acids in with or without heat stress condition. RESULTS: Results demonstrated the level of expression of cytochrome P450 in broilers fed zeolite (Z) in the 21st day of experiment, silver nanoparticles coated with zeolite in combination with organic acid (NSOA) in 42nd day without heat stress condition and silver nanoparticles coated with zeolite (NS) in day 42 with heat stress condition in intestine and liver had significant increase in expression in comparison with control (C) treatment (P<0.05). CONCLUSIONS: In conclusion, result showed that zeolite and silver nanoparticles were identified as inorganic, chemical and exogenous substances in liver and intestine and up-regulated expression of pollution biomarkers genes. Whereas this effect is not identified in organic acid.

BACKGROUND: Antioxidant compounds and unsaturated fatty acids play an important role in improving the immune response and intestinal microflora in broiler chickens. Objectives: The aim of this study was to investigate the effects of different sources of antioxidant and soybean and rapeseed oils on humoral immune responses, intestinal microflora and some of the blood parameters of broiler chicken. Methods: A total of 480 male broiler chicks (Ross 308) were randomly allocated in a factorial arrangement (2×5) based on a completely randomized design with 10 treatments and 4 replicates during 42 days of age. Experimental factors consisted of different sources of antioxidant (rosemary, thymus and satureja essential oils at level of 300 ppm and vitamin E at concentration 200 mg/kg and without antioxidant) and vegetable oils (soybean and rapeseed oils at concentration of 4 percent of diet). Immune characteristics and intestinal microflora population were evaluated. Results: The use of different soybean and rapeseed oils had no significant effect on antibody titer (P>0.05) but the use of antioxidants improved immune responses against Newcastle disease (P<0.01). Also, the use of rosemary, thymus and satureja essential oils increased serum level of blood high density lipoprotein and reduced blood low density lipoprotein (P<0.001).  Furthermore, the concentrations of cholesterol and triglyceride increased in without  antioxidant treatment (P<0.001).Also, use of antioxidant treatments caused significant decrease of heterophil to lymphocyte ratio (P<0.05), then these result are consistent with the immune response against Newcastle disease. Furthermore, escherichia coli to lactic acid bacteria ratio increased in rosemary and thymus essential oils treatments compared with without antioxidant treatment (P<0.05). Conclusions:The use of dietary soybean and rapeseed oils did not have a significant effect on different traits of broiler chicken, but adding dietary antioxidants improved immune responses and intestinal microflora and in between different antioxidants, the use of essential oils compared with vitamin E  induced significant improvement of traits.

BACKGROUND: Barley cultivars contain different anti-nutritional factors that have negative effects on the intestinal ecosystem and reduce blood cholesterol concentration. Objectives: This study aimed to determine the effect of processing barley cultivars on the intestinal morphology, enzyme activity and volatile fatty acids of the small intestine and serum lipid levels of broiler chickens. Methods: The experiment was carried out using a factorial experiment with a completely randomized design. Factors were barley cultivars (Fajr, Reyhaneh, and Yosef), diet form (mash or pellet) and enzyme level (0 and 0.05 g/kg dry matter). Around 927 one day of old Ross 308 broiler chickens were assigned into 12 treatments, 3 replicates and 27 chickens in each replicate. Results: Pancreatic amylase, lipase enzyme activity and digesta pH are affected by main (except for cultivar) and interaction effects (P< 0.05). Feed Processing (pelleting, adding enzyme or combination of pelleting and enzyme) of barley cultivars, especially diets containing Ryhaneh barley cultivar reduced digesta viscosity and increased the concentration of volatile fatty acids and relative percentage of acetic acid and butyric acid (P<0.05). Diets containing Fajr barley cultivars, enzyme or pelleted diets significantly decreased blood glucose, cholesterol and HDL concentration (P<0.05). Conclusions: Due to lower amount of soluble NSP compared with the other barley cultivars, Ryhaneh cultivar resulted in a better response to processing as well as enzyme addition, and improved the morphological characteristics of the small intestine

BACKGROUND: Leucine is one of the subgroups of amino acids, which play an important role in the anabolism of muscles, adipose tissue, and the liver by stimulating insulin secretion.OBJECTIVES: Effects of different levels of leucine were investigated on carcass yield, characteristics, and quality, and expression of insulin-like growth factor (IGF-1) and insulin genes in male broilers.METHODS: Five levels of L-leucine (100, 110, 120, 130, and 140 % of Ross strain requirements) were tested with 250 male one-day-old chicks in a completely randomized design with five treatments and five replicates (containing 10 chicks each). On day 42 of their age, the blood samples of two birds from each replicate (10 birds per treatment) were taken to determine serum IGF-1 gene expression. Subsequently, these birds were slaughtered for analysis of carcass characteristics and quality, and collecting the samples of liver and breast for expression of IGF-1 and insulin genes.RESULTS: Body weight increased by consumption of 140 % of leucine as compared to 100 %. Reduction in feed conversion ratio was observed by feeding 140 % of leucine level. The IGF-1 gene expression of breast and liver increased by 110 % of leucine level. Moreover, feeding 110 % of leucine level caused a higher expression of insulin gene in breast and liver. Consumption of 130 % of leucine improved the meat protein, fat, and ash contents. Furthermore, consumption of 110 % of leucine increased the serum IGF-1 concentration.CONCLUSIONS: Consumption of leucine in broiler diets was found to increase the expression of IGF-1 and insulin genes and consequently, improve the performance and carcass quality. It also decreased the abdominal fat in broiler chickens.

BACKGROUND: Antibacterial drugs have long been used for prevention and treatment of poultry diseases but their misuse or overuse can make adverse effects on public health including occurrence of drug residues in poultry products. OBJECTIVES: To assess the frequency and status of antibacterial drug consumption in broiler production farms in Qum province. METHODS: In the present survey, Qum province was divided into six regions (north, west, southwest, south, southeast and east) and in total 138 broiler production units (59%) were studied by direct interview using a questionnaire. RESULTS: The present study showed that the most frequently used antimicrobial drugs in broiler farms were  sulfamethoxazole+trimethoprim (93.4%) followed by enrofloxacin (60.0%), colistin (49.7 %), furazolidone (42.0 %), oxytetracycline (17.5 %), and chloramphenicol (14.6 %). Mean antibacterial consumption rate during a 42-48 day production period was 3.0± 0.37 times per farm. A notable finding in this survey was the high percentage of banned drug usage such as furazolidone and chloramphenicol, indicating the ignorance or unawareness of poultrymen regarding the potential hazards of these drugs on public health. CONCLUSIONS: Owing to widespread and frequent usage of antibacterial drugs in broiler farms, all-out actions are needed to be taken in educational, research and administrative fields of veterinary and animal production sectors for rational and responsible use of these drugs in poultry industry.

BACKGROUND: AmpC and ESBLs as mediated-plasmid extended spectrum β-lactabases are the main factors of resistance to extended-spectrum cephalosporins in enterobacteriacea especially E. coli and will follow treatment failure, high costs of treatment in human and economic losses in the poultry industry. OBJECTIVES: The purpose of this study was to screen and study the faecal E. coli isolates producing extended spectrum β-lactamases (ESBLs) and AmpC enzymes and related workers. METHODS: A total of 500 cloacal swab samples from broiler chickens and 25 rectal swab samples from workers were collected from five poultry houses around Tehran. All samples were seeded on MacConkey agar and identification of E. coli isolates were performed via biochemical tests. Antibiotic susceptibility was determined against 12 antibiotics using the disk diffusion method as recommended by the clinical and laboratory standard institute (CLSI2012). Ceftazidim / ceftazidim-clavolanic acid and cefoxitin / cefoxitin-EDTA disks were used for the detection of ESBL and AmpC phenotypes, respectively. phonetic analysis of the drug resistances was performed via SPSS software and Chi-square test. ESBL- producing E. coli screened by PCR for the presence of genes encoding beta-lactamases of TEM, CTX-M and SHV.       RESULTS: A total 467 E. coli isolates were isolated from 88.9% of the samples as 92% and 72.7% of isolates presenting MDR phenotype among chickens and workers respectively. ESBL phenotype detected in 5.5% (26) of poultry isolates while, none of the workers isolates have this phenotype. Six isolates carried both of TEM and CTX-M whereas, five and one isolates were detected only for TEM and CTX-M, respectively. Eighty-eight and nine-tenths percent of ESBL E. coli displayed AmpC phenotype. CONCLUSIONS: Since cephalosporins are not used in broilers in Iran, isolation of faecal E. coli isolates producing extended spectrum β-lactamases in broilerchickens can indicate transfer of the resistance genes via plasmids and other mobile genetic elements among Enterobacteriaceae.

BACKGROUND: Population growth and the need for food, especially proteins, have increased the interest in using inexpensive and available supplements to increase protein production. ObjectiveS: The aim of this study was to evaluate the effect of Qom region zeolite as a supplement to the histological and histochemical structure of broiler chicken intestine and to compare it with commercial zeolite. Methods: In this experimental study a total of 200 broiler chicks were purchased from Qom poultry and randomly divided into five groups including the control group; the experimental group of Qom zeolite which was subsequently subdivided into three different doses including (Z1 = 0.5 %, Z2 = 1%, Z3 =1,5%); and finally the anzymite group which received the commercial zeolite. 42 days later, 10 samples were selected from each group and after tissue preparation, the slides were stained with hematoxylin-eosin, PAS period, and toluidine blue; then microscopic assays were applied using the Image-j 1.49f software. Data analysis was done with SPSS 20 and One-way ANOVA and Tukey’s post hoc test. PResults: The height of the epithelial cells decreased in the anzymite group but the number of mucosal glands and goblet cells increased significantly (P<0.05). Except for Z1 group in other Qom zeolite treatment groups (Z2 and Z3), the number of goblet cells increased significantly (P<0.05). Therefore, the effect of zeolite higher than 1.5% may be comparable with anzymite. Conclusions: Our results showed that zeolite does not have any effect on increasing the absorption rate of the small intestine, but could stimulate the function of goblet cells and intestinal glands, so Qom zeolite with higher percentage could have the same effect as anzymite

The current work aimed to study the phenotypic and genotypic characters of oxidase positive Gram negative bacterial pathogens recovered from different pathological lesions in broiler chickens. Samples were taken from 200 Hubbard and Ross broiler chickens of different ages (3-5weeks), from different farms in Beni-Suef and El-Fayoum Governorates during the period from January 2016 to April 2016. Bacteriological examination showed that Gram negative bacteria were 165 (82.5%) of isolates of which 60 isolates (30%) were oxidase negative while 105 isolates (52.5%) were oxidase positive including 43 Pseudomonas aeruginosa, 35 Aeromonas hydrophila, 12 Pasteurella gallicida, 10 Plesiomonas shigelloides, and 5 Vibrio vulnificus with incidences of 21.5%, 17.5%, 6% 5%, and 2.5%, respectively. The in-vitro sensitivity tests were applied on a total of 59 isolates; 20 P. aeruginosa, 19 A. hydrophila, 10 P. gallicida, 5 P. shigelloides and 5 V. vulnificus against 13 different antimicrobial agents and multidrug resistant isolates were detected. Multiplex-PCR was applied on 15 different MDR isolates. The results of PCR revealed that blaTEM, CIT and FOX genes were the most prevalent where they were found in 8 isolates (53.3%) followed by blaSHV which was found only in 5 isolates (33.3%)

Bacterial disease still has serious problem in the intensive poultry production. In the recent years, particular concern has been raised by high incidence of poultry infections by E. coli. Analyses of antibacterial properties of essential oils have been carried out by range of researches. This experiment aimed to study the effect of immunomodulators on the immunogenicity of vaccine E. coli O78. In this study 250 broiler chickens were used. They were divided into 5 separated groups all groups vaccinated with E. coli O78 cebel coarse spray vaccine, except control group, 2 groups treated with immunomodulators, 3 groups challenged with untyped E. coli strain, all chickens housed in separated anavar. First group was control, 2nd group was vaccinated only, 3rd group was vaccinated and challenged, 4th group was vaccinated and received immunomodulators and 5th group was vaccinated, received immunomodulators and challenged. All chickens were observed daily food consumption, weight gained mortality rate, lesion, bioavailability, and weekly collected blood samples from 2-5 birds. The results were summarized as follows; immunomodulators have positive effect on B.W.G, decreased mortality and morbidity rate. The challenge enhanced the effect of E. coli O78 vaccine and there was marked improvement in bioavailability, B.W.G and immune defense against bacterial and respiratory diseases. Also, immunomodulators increased immunogenicity against bacterial disease through enhancing immune response system, and had synergistic effect with vaccination against E. coli.