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A study of oocyst shedding pattern and weight changes in neonatal calves experimentally infected with Cryptosporidium parvum
2015
Zarghami, Faisal | Mokhberdezfouli, Mohammadreza | Rahbari, Sadegh | Shayan, Parviz | Ebrahimzadeh, Elahe | Boloorchi, Mamood | Lotfolahzadeh, Samad
BACKGROUND: Cryptosporidium parvum is a protozoan parasite which belongs to apicomplexa phylum. The parasite infects both wild and domesticated animals and human beings as well. OBJECTIVES: The purpose of the present study was to detect oocyst shedding and diarrhea pattern in experimental cryptosporidiosis and their correlation with weight loss in neonatal calves. METHODS: Twelve Holstein calves of both sexes were obtained at birth from dairy farm and randomly divided into two groups of 6 calves. Six calves were orally infected with 107 C.Parvum oocysts at the 12h post parturition. The control group was not infected. Clinical signs were examined and fecal samples were collected by the rectal examination twice a day. All calves were weighed from day 0 to day 30 with 3 days intervals to determine effects of cryptosporidiosis on weight gain. RESULTS: All infected calves were noticeably depressed and had a decreased appetite from 3 days post inoculation (DPI) while they received colostrum. Subsequently, watery diarrhea with clumps of mucus and yellow or pale changes of feces color were observed. The infected calves have had diarrhea for 5-8 days that remarkably had got dehydrated. The most severity of diarrhea was 4-6 DPI. Oocyst excretion started 4 DPI, peaked at 6 DPI (60.48×106±9.03oocysts/g feces) and continued until 11 DPI. Control calves had no diarrhea and other clinical signs during the whole period of the trial. The mean weight gain of control group was significantly higher than inoculated group during experiment (p<0.001). The Weight of the infected calves was retarded until 9 days old and then risen subsequently. CONCLUSIONS: Present study showed the role of C.Parvum as the primary cause of diarrhea and weight loss among neonatal calves.
Mostrar más [+] Menos [-]The effect of dystocia on physiological and behavioral characteristics related to vitality and passive transfer of immunoglobulins in newborn Holstein calves
2015
Murray, Christine F. | Veira, Doug M. | Nadalin, Audrey L. | Haines, Deborah M. | Jackson, Marion L. | Pearl, David L. | Leslie, Ken E.
The objective of this study was to examine the effect of calving difficulty or dystocia on the vitality of newborn calves and its association with blood pH, the apparent efficiency of immunoglobulin G (IgG) absorption (AEA), and weight gain. A total of 45 calving events (N = 48 calves) were monitored from the first sight of fetal membranes. All calves were assessed at the time of first attaining sternal recumbency (SR), at 2 and 24 h, and at 7 and 14 d of age. Measurements included time to SR, rectal temperature, respiration and heart rate, analysis of blood gases and other blood measures, suckling response, time to standing, passive transfer of IgG, and weight gain. Calves were separated from their dam 2 h after birth and fed a commercial colostrum replacer containing 180 g of IgG by esophageal tube feeder. Calves born following dystocia had lower venous blood pH and took longer to attain SR and attempt to stand than those born unassisted. Duration of calving interacted with the number of people required to extract the calf by pulling as a significant predictor of pH at SR. No association was found between pH at SR and AEA. However, reduced AEA was found in calves that were female and in calves that did not achieve SR within 15 min of birth. A longer calving duration, being born in July or August rather than June, and a shorter time spent standing in the first 2 d of life were significantly associated with reduced weight gain to 14 d. It was concluded that factors at calving impact the physiology, vitality, and subsequent weight gain of newborn calves.
Mostrar más [+] Menos [-]Effects of isoflurane on somatosensory-evoked potentials in calves: A pilot study
2015
Truchetti, Geoffrey | Burns, Patrick | Nichols, Sylvain | Parent, Joane
Somatosensory evoked potentials (SSEP) are used to monitor sensory function and are often recorded under general anesthesia. The objective of the study was to evaluate the effects of isoflurane on SSEPs in calves as it has not been reported. Eight calves (mean age: 40 days), were included in the study. Calves were anesthetized with a randomized sequence of four different isoflurane partial pressures. Blood gas analysis was performed before each measurement. SSEP were induced by repeated stimulation of the common dorsal digital nerve III. SSEPs were recorded from the lumbo-sacral junction (s-SSEP) and the head (c-SSEP). Latency and inter-amplitude of each peak were measured. For s-SSEP: One negative (Nsp1) and two positive (Psp1 and Psp2) peaks were identified in all tracings except for two calves. There was a significant effect of isoflurane on the latency of Psp2 (P = 0.01). Inter-amplitude decreased significantly with PaO2, PaCO2 and temperature (P < 0.05). Psp2 latency decreased with PaO2 (P = 0.01). For c-SSEP: two positive (Pc1 and Pc2) and two negative (Nc1 and Nc2) peaks were identified. There were identifiable peaks for the analysis of Pc1 latencies only. There was a significant positive linear relation between end-tidal isoflurane partial pressure (ETiso) and Pc1 latency (P = 0.04). None of the co-variables had a significant effect on the latency of Pc1 (P > 0.1). Isoflurane has a major impact on the recording of c-SSEP. Recording should be done at the lowest ETiso as possible, and anesthesia parameters should be kept constant.
Mostrar más [+] Menos [-]Immunohistochemical expression of nuclear factor erythroid-2-related factor 2 and heme oxygenase 1 in normal bovine lung and bovine lung infected with Mannheimia haemolytica
2015
Moussa, Amira Talaat | Balajīta Siṅgha, | Al-Dissi, Ahmad N.
Mannheimia haemolytica is an important cause of pneumonia in feedlot cattle. Nuclear factor erythroid-2-related factor 2 (Nrf2) is a redox-sensitive transcription factor responsible for the induction of antioxidant enzymes, such as heme oxygenase 1 (HO-1), within the lung. The expression of Nrf2 and HO-1 was immunohistochemically evaluated in 4 calves 24 h after experimental infection with M. haemolytica. Calves receiving normal saline served as controls. In the infected lungs, cytoplasmic Nrf2 expression was high in macrophages and bronchioles and low in alveolar epithelium, whereas nuclear expression was high in endothelial cells, macrophages, and bronchioles and lowest in alveolar epithelium. Normal lung samples displayed only faint Nrf2 cytoplasmic staining within bronchiolar epithelium. Expression of HO-1 was detected within the cytoplasm of macrophages and bronchiolar epithelial cells in all infected lung samples, whereas normal lungs displayed only weak cytoplasmic staining in bronchiolar epithelial cells. These findings suggest that bronchiolar epithelial cells and macrophages up-regulate Nrf2 expression early in the course of infection, which results in increased expression of HO-1 within these cells.
Mostrar más [+] Menos [-]Comparative efficacy between ciprofloxacin and gentamycin in neonatal calves in Bangladesh
2015
Hossain, M.K | Islam, M.S. | Alam, M.S | Uddin, A.S.M.A | Uddin, A.H.M.M. | Hossain, M.M. | Lucky, N.S
Diarrhoea in calves is considering a serious clinical problem inBangladesh. Calf is the back bone of the dairy industry. Diarrhoea in farm animals, especially in neonatal calves is one of the most challenging clinical syndromes encountered by large animal Veterinarypractitioners. The present investigation was conducted with the aimed to evaluate the efficacy of ciprofloxacin and gentamycin in the treatment of diarrhoea in neonatal calves. The studied was carried out for a period of six month from January 2013 to June 2013 in Kanaighat, Sylhet, Bangladesh. Calves (n=60) under 10 days of age with clinical signs of diarrhoea were randomly divided into two groups. One group was treated with intramuscular ciprofloxacin at a dose of 5 mg/kg once daily, while other group was treated withintramuscular (IM) gentamycin (dose of 5 mg/kg) once daily for three consecutive days. Calves evaluated clinically from the treatment and the recovery rates for the ciprofloxacin and gentamycin groups afterthree days of treatment were 73.33% and 66.67%, respectively. This trial revealed ciprofloxacine administered through IM route from the first day of the clinical sign observed was more efficacious in calfdiarrhoea compared to gentamycin.
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