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Hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in Sri Lanka
2019
Nadeesha H. N. Dilrukshi | Anura P. Jayasooriya | Gamika A. Prathapasinghe
Objective: This study was conducted to determine hematological reference ranges and morpho¬logical characteristics blood cells in healthy captive Sri Lankan fresh water turtle and tortoise species. Materials and Methods: Following turtle species, namely, Melanochelys trijuga parkeri (n = 06), M. trijuga thermalis (n = 06), and Lissemys punctata punctata (n = 06) and a tortoise species Geochelone elegans (n = 06) were evaluated. Blood smears were stained using LeishmanGram staining protocol. The differential white blood cell counts and morphology were assessed using the standard protocols. Results: Both red blood cells and their nuclei were irregular in M. trijuga parkeri, whereas M. trijuga thermalis had oval-shaped and the nuclei were irregular in shape. L. punctata punctata and G. elegans had oval-shaped red blood cells and their nuclei were round in shape. In terms of differential counts, heterophils were the most abundant leukocyte type in all the species. Melanochelys trijuga parkeri had the highest heterophils value of 55% and the lowest value of 48.5% was recorded with G. elegans. Lymphocytes count was significantly high in G. elegans. Characteristic morphological features were observed in different leukocytes. In terms of thrombo¬cytes, M. trijuga thermalis and L. punctata punctata had single platelets, whereas M. trijuga park¬eri and G. elegans had platelets as clumps. Thrombocytes are ellipsoidal with centrally located dark-stained nuclei and their cytoplasm is clear. Conclusion: The findings in this study can be used as the reference values in the assessment of health of the above species and also will be useful in future hematological studies related to these species. [J Adv Vet Anim Res 2019; 6(3.000): 266-271]
Mostrar más [+] Menos [-]SERUM BIO-CHEMICAL INDICES IN CAPTIVE BONNET MACAQUES (Macaca radiata)
2024
M. Palanivelrajan | M.G. Jayarthangaraj | R. Sridhar | M. Parthiban | R. Thirumurugan
A clinical parameter study was conducted to file the reference serum bio-chemical values of Bonnet macaques (Macaca radiata) reared under captivity. The animals were chemically restrained and six blood samples (3 males and 3 females) were collected from the saphenous vein and processed. In this study, the mean ± S.E. of estimated value of serum biochemical profiles were close to the normal range reported earlier. But, serum glucose level was less and serum level of phosphorus was comparatively high.
Mostrar más [+] Menos [-]Eperythrozoonosis (Mycoplasma sp.) in Malaysian Pangolin
2014
Chandrawathani P. | Faizal H. | Erwanas A. I. | Lily Rozita | Jamnah O. | Ramlan M. | Premaalatha B.
The Malaysian Pangolin (Manis javanica) is an endangered species,
but is widely hunted for its medicinal value in body parts. A total of sixteen pangolins were screened for blood protozoa and six
pangolins were confi rmed to be positive for eperythrozoonosis infection based on morphology from blood smears stained with 8% Giemsa. The causative organism, Eperythrozoon sp., with a size of 0.3 μm were observed under a compound microscope at 100× magnification on the surface of red blood cells as blue coloured dots. This is the fi rst report of Eperythrozoon infection from pangolins in Malaysia. Further identifi cation using polymerase chain reaction (PCR) is necessary to confi rm E. ovis or wenyonii,
which is uncultivatable in artifi cial media.
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