Different concentrations of artemisia extract were experimentally tested to treat Lernaea and Argulus infestations in common carp (Cyprinus carpio) fish with different methods. A total number of 210 carps (50 ± 10 g) were collected from private farms at Kafr El-Sheikh Governorate, Egypt, and divided into 7-groups in triplicate: G1 negative-control (apparently healthy carps), G2 positive-control (infested carps with Lernaea and Argulus), G3: infested carps treated with dipping in a NaCl solution (5g /L for 5 min. for 3 consecutive days), G4-G5: infested carps treated with dipping in a solution of artemisia extract with concentration 50% and 60% respectively, for 5 minutes, then fishes were accommodated at a recovery tank and inspected for 10 min., G6-G7: infested carps were placed in aquarium supplied with artemisia extract (3 mg/ L and 6mg/L respectively, for 15 consecutive days). The results revealed that artemisia extract had a noticeable positive effect on survival, recovery of infested carps and the parasitic elimination% which increased proportionally with increasing the concentration of the extract, whereas the best results were obtained at G5. Also, there were an improvement in serum biochemical parameters (increased total protein, albumin and globulin with reduced AST, ALT, creatinine and urea), which were proportionally improved with increasing both concentration and the duration of exposure to the extract, (especially at G7). Gross and histopathological examination and alterations of skin, gills, liver and intestine also revealed a noticeable improvement to the normal in the groups treated with artemisia extract in comparison with G2 especially G6 and G7. Consequently, application of artemisia extract is a safe and optimal method for treatment of Argulus and Lernaea parasitism in cultured carp.

Objective: To evaluate the effect of water temperature on intramuscular injected alfaxalone anesthesia in carp (Cyprinus carpio). Materials and Methods: Six healthy adult carp (C. carpio) were intramuscularly injected with alfaxalone (2.5, 5.0, or 7.5 mg/kg) at normal water temperature (25°C) and at low water temperature (2.5 mg/kg, 15°C). The respiratory rate, heart rate (HR), and anesthesia depth (AD) were evaluated every 5 min for 30 min after administration and every 1 h after 60 min after injection. Results: The respiratory and HRs did not change significantly upon alfaxalone injection, regardless of dose. However, a dose-dependent increase in AD scores was observed. Furthermore, 2.5 mg/kg alfaxalone injected in 15°C water showed an almost equal anesthetic effect to that of 5.0 mg/kg alfaxalone in 25°C water. Conclusion: Alfaxalone is readily available, and its anesthetic effect in carp was enhanced by lowering water temperature, illustrating the possibility of intramuscular injection of alfaxalone in fish. [J Adv Vet Anim Res 2024; 11(1.000): 27-32]