Cellulitis is a serious problem for the poultry industry because of increased condemnations, carcass downgrading at processing, and higher labor costs to process affected flocks. In the present study, the prevalence of cellulitis was studied in 240 broiler chickens. The correlation between cellulitis and other systemic lesions of the same bird was investigated also. Moreover, identification of the causative bacterial agents was conducted focusing on E. coli and Salmonella isolates. The prevalence rate of cellulitis in examined broiler chickens was 38.3%. Cellulitis without systemic lesion was observed in 14.2% of birds while 24.2% of birds had cellulitis associated with other systemic lesions in the internal organs while hepatitis was the most frequent. The bacteriological examination revealed that of 253 samples collected, a total of 157 bacterial isolates were recovered (62.1%). Among the recovered isolates, E. coli was the most prevalent (126 isolates; 80.3%) as well as 4 Salmonella species (2.5%), 9 Proteus species (5.7%), 7 Pseudomonas aeruginosa (4.5%), 3 Enterobacter species (1.9%) and 8 Staphylococcus aureus (5.1%). Serogrouping of E. coli isolates revealed that O125 was the most prevalent; 32%, followed by serogroups O158, O55, O78 as 24%, 12%, 10%, respectively, then both O1 and O8; 6% for each, and finally O15; 4%. Antibiogram of E. coli isolates showed a high sensitivity against enrofloxacin only (81%) while they were moderately sensitive to apramycin (65.9%) and colistin sulphate (61.9%) as well as ciprofloxacin and cefotaxime sodium (56.3% and 55.6%, respectively). On the other hand, high moderate degrees of resistances were observed against the other antimicrobials. Salmonella isolates showed complete sensitivities to ciprofloxacin and enrofloxacin while they were completely resistant to most of antimicrbials.

Chromobacterium violaceum is a facultative anaerobic, gram-negative rodshaped bacteria normally found in soil and stagnant water of tropical and subtropical countries. Infections of Chromobacterium violaceum are rare among mammals, but the first human infection were reported in Malaysia in 1927. This clinical case reports two separate cases of Chromobacterium violaceum infection in two sub-adult male Black-handed Gibbon (Hylobates agilis). Both animals were presented with the history of diarrhea, pyrexia and inappetance. From the physical examination, the findings were high body temperature, dull, inactive, soft fecal stained at the rectum and small open wound at lower limb of one of the case. The treatment given was antipyretic and multivitamin. Unfortunately, due to poor prognosis both patients died within 48 hours after presentation and treatment. Autopsy examinations wereperformed to find out the cause of death. Post mortem examination findings revealed hepatomegaly with multiple size whitish-yellow spots on the liver surface, congestion and enlargement of spleen and lymph node, congestion of lung and loss of demarcation between renal cortex and medulla of the kidney. The cellular changes via histopathological findings of kidney, spleen, lung and liver were suggestive of septicaemia. The gross and histopathologicalfindings were supported by the isolation of Chromobacterium violaceum via bacterial isolation and identification from lung, liver, spleen and kidney. Thus, the cause of death of the two sub-adult male Black-handed gibbon in this case are due to septicaemiadue to Chromobacterium violaceum infection.

Different types of skin lesions and their distribution in dogs withrecurrent pyoderma along with haematobiochemicalfindings were recorded in this study. Dogs with recurrent superficial pyoderma revealed papules, pustules, crusted papules, erythema, alopecia,crusts, scales, plaques, hyper-pigmentation and pruritus. Dogs affected with recurrent deep pyoderma had symptoms like papules,pustules, cellulitis, ulcers, crusted papules, nodules, fistulous tracts, alopecia, scale formation, crusts, hyper-pigmentation,erosions and furunculosis, pain and edema. The major locations of lesions for recurrent superficial pyoderma included lateral abdomen, lateral thorax and dorsum, axilla, groin, hind limb, foot, neck and fore limb and head. Lesions of recurrent deep pyoderma were predominantly observed over dorsum and lateral abdomen followedby head, neck, hind limb, lower abdomen, axilla and groin, forelimb and lateral thorax. Haemato-biochemical findings revealed leucocytosis, increased in absolute neutrophil count, eosinophil count and high serum cholesterol levels. Affected dogs also had decreased haemoglobin concentration, total erythrocyte count and serum albumin levels.

This study established a disease model and protocol for bacterial challenge with Escherichia coli serogroup O2 strain EC317 in Japanese quail. Five groups of 10 birds each were injected subcutaneously in the breast with 200 μL of a brain–heart infusion (BHI) culture containing 1 × 10(8), 1 × 10(7), 1 × 10(6), 1 × 10(5), or 1 × 10(4) colony-forming units/mL of the test organism, which had been isolated from a turkey with cellulitis and septicemia. Birds in a 6th group were controls that received sterile BHI alone. Localized lesions of cellulitis developed in all of the birds that received E. coli. The morbidity and mortality rates were highest (100%) in the birds receiving the highest dose of E. coli and decreased linearly with decreasing dose (P < 0.05). Severity of disease, including lesions of pericarditis and perihepatitis, was also directly proportional to the dose of E. coli. These findings indicate that this disease challenge protocol can be used to study disease resistance and immunologic consequences of contaminant exposure or other stressors in birds.

Avian cellulitis is one of the most important field problems facing the poultry sector. Sever financial losses resulted from the condemnation of the broiler carcasses infected with cellulitis lesions. In light of this, the current study was aimed to isolation and identification of the bacterial species causing cellulitis in broiler chickens in Dakahlia and Sharkia Governorates, Egypt. The bacterial isolates were tested for their antimicrobial susceptibility and molecular detection of some virulence and antimicrobial resistance genes. In addition to evaluate the antibacterial activity of quaternary ammonium compounds and glutaraldehyde (TH4 ®) against the bacterial isolates. Four bacterial species were isolated; E. coli, S. aureus, P. aeruginosa and Proteus mirabilis with percentages of (75%), (20%), (6%) and (5%) respectively. E. coli was recorded as the most predominant isolated bacteria in this study with12 different sero- groups (O1, O2, O26, O55, O78, O91, O121, O125, O126, O128, O153 and O158). E. coli O78 and O91 were the most prevalent identified sero- groups.  The antimicrobial susceptibility testing revealed higher resistances against doxycycline and ampicillin (95.6%), amoxicillin (90.7%), norfloxacin (84%), oxytetracycline (79.1%) and amikacin (71.6%) in E. coli, doxycycline (73.3%), oxytetracycline (80%)            ,ampicillin(75%), streptomycin (80%), erythromycin (73.3%), and oxacillin (86.7%) in S. aureus, doxycycline (83.3%), oxytetracycline (77.8%), ampicillin (83.3%), amoxicillin (88.9%), neomycin (72.2%) and erythromycin (77.8%) in P. aeruginosa and doxycycline, oxytetracycline, ampicillin, amoxicillin, streptomycin and erythromycin (100% for each of them) in Proteus mirabilis. All isolated bacterial species were multidrug resistance (MDR). The molecular identification showed the detection of virulence genes: iutA in E. coli, nuc in S. aureus, toxA in P.aeruginosa and rsbA in Proteus mirabilis., with percentage of (100%). blaTEM, tetA (A), qnrA, tetK, mecA, aac(6')aph (2''), ereA and aada1 resistance genes were  reported in this study. Quaternary ammonium compounds in combination glutaraldehyde (TH4 ®) with 2% concentration showed the highest antibacterial activity against the examined multidrug resistant bacterial isolates. These results suggested for application of 2% TH4 ® to achieve effective disinfectant programs in poultry farms.