Refinar búsqueda
Resultados 21-26 de 26
Bacteriologic and pathologic studies of hepatic lesions in sheep
1990
Scanlan, C.M. | Edwards, J.F.
At an abattoir, lesion specimens from 140 condemned sheep livers were collected for bacteriologic culture and for pathologic examination. Grossly, 23 lesions were abscesses; from 9 of which, Fusobacterium necrophorum biovar A (3 in pure culture and 6 in mixed culture) was isolated and from 14 of which, biovar B (6 in pure culture and 8 in mixed culture) was isolated. Escherichia coli was the predominant facultative anaerobic bacterium and Clostridium perfringens was the predominant obligate anaerobic bacterium isolated from the 14 lesions with mixed bacterial infection. Histologically, these lesions had a core of coagulation necrosis, encircled by a zone of necrotic phagocytic cells and bacteria with cellular characteristics of F necrophorum biovars A or B, and a connective tissue capsule. Of the 117 lesions without F necrophorum, 49 were culture-positive (for other organisms) and 69 were culture-negative. These 117 lesions were fibrous and were smaller than the 23 abscesses. A variety of gram-positive and gram-negative facultative anaerobic and obligate anaerobic bacteria was isolated from the culture-positive lesions, but always in low numbers. Eleven culture-negative and 18 culture-positive lesions were examined and had histologic characteristics of parasite-induced granulomas, with numerous eosinophils and epithelioid giant cells. Results of the study indicated that the histologic appearance of ovine hepatic lesions with F necrophorum was similar to bovine liver abscesses caused by F necrophorum, but unlike bovine liver abscesses, F necrophorum biovar B was isolated more frequently than was biovar A and often in pure culture. Most of the lesions in the condemned livers were parasite-induced granulomas.
Mostrar más [+] Menos [-]Evaluation of inhibitor activity of bacterial sialidase from Clostridium perfringens against Newcastle disease virus in the cell culture model using chicken embryo fibroblast
2022
Ryan Septa Kurnia (Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia) | Rahajeng Setiawaty (National Veterinary Drug Assay Laboratory (NVDAL), Raya Pembangunan Gunung Sindur, Bogor, Indonesia) | Ketut Karuni Nyanakumari Natih (National Veterinary Drug Assay Laboratory (NVDAL), Raya Pembangunan Gunung Sindur, Bogor, Indonesia) | Christian Marco Hadi Nugroho (Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia) | Otto Sahat Martua Silaen (Animal Health Diagnostic Unit, PT. Medika Satwa Laboratoris Kp. Kayumanis, Bogor, Indonesia) | Silvia Tri Widyaningtyas (Institute of Human Virology and Cancer Biology Universitas Indonesia, Jakarta, Indonesia) | Simson Tarigan (Indonesian Research Centre for Veterinary Science, Bogor, Indonesia) | Fera Ibrahim (Department of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia) | Pratiwi Pudjilestari Sudarmono (Department of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.)
Objective: The Newcastle disease virus (NDV) is an infectious disease that causes very high eco¬nomic losses due to decreased livestock production and poultry deaths. The vaccine's ineffec¬tiveness due to mutation of the genetic structure of the virus impacts obstacles in controlling the disease, especially in some endemic areas. This study aimed to provide an alternative treatment for NDV infection by observing the viral replication inhibitor activity of Clostridium perfringens sialidase in primary chicken embryo fibroblast (CEF) cells. Materials and Methods: The virus was adapted in CEF monolayer cells, then collected thrice using the freeze–thaw method and stored at −20°C for the next step in the challenge procedure. C. perfringens crude sialidase was obtained, but it was further purified via stepwise elution in ion exchange using Q Sepharose® Fast Flow and affinity chromatography with oxamic acid agarose. The purified sialidase was tested for its toxicity, ability to breakdown sialic acid, stopping viral replication, and how treated cells expressed their genes. Results: According to this study, purified C. perfringens sialidase at dosages of 187.5, 93.75, and 46.87 mU effectively hydrolyzes CEF cells' sialic acid and significantly inhibits viral replication on the treated cells. However, sialidase dosages of 375 and 750 mU affected the viability of mono¬layer CEF cells. Interestingly, downregulation of toll-like receptor (TLR)3 and TLR7 (p < 0.05) in the sialidase-treated group indicates viral endocytosis failure. Conclusions: By stopping endocytosis and viral replication in host cells, sialidase from C. perfrin¬gens can be used as an alternative preventive treatment for NDV infection. [J Adv Vet Anim Res 2022; 9(2.000): 335-345]
Mostrar más [+] Menos [-]Studies on the Clostridium perfringens type C infection of pig in Korea
1993
Yeh, J.K. | Park, K.Y. (Bayer Veterinary Medical Research Institute, Seoul (Korea Republic)) | Cho, S.K. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
Rapid identification and toxin type analysis of Clostridium perfringens isolated from healthy or diseased stocks with necrotic enteritis in chicken
1997
Kim, H.J. | Chung, U.I. (Miwon Institute of Animal Science, Ichon (Korea Republic)) | Kang, M.I. (Chonnan National University, Kwangju (Korea Republic). College of Veterinary Medicine)
Studies on the necrotic enteritis of chicken in Korea
1994
Park, K.Y. | Jung, S.D. | Yeh, J.G. (Bayer Veterinary Medical Research Institute, Anyang (Korea Republic)) | Seo, I.B. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
Clostridium perfringens e a enterite necrótica em frangos: principais fatores de virulência, genéticos e moleculares | Clostridium perfringens and necrotic enteritis in poultry: virulence, genetic and molecular factors
2014
Luis Antonio Llanco Albornoz | Viviane Nakano | Mario Julio Avila-Campos
<em>Clostridium perfringens</em> é o causador da enterite necrótica que afeta a produção de frangos de corte no mundo todo. Essa bactéria produz diversas toxinas e causa lesões no intestino tendo como consequências a elevada mortalidade e perdas econômicas devido à baixa produtividade. Nessa revisão são apresentados os principais fatores de virulência, a susceptibilidade aos antimicrobianos e a diversidade genética de <em>C. perfringens</em> isolados de frangos com enterite necrótica. | <em>Clostridium perfringens</em> cause necrotic enteritis affecting the poultry production worldwide. This bacterium produces various toxins and causes lesions in the intestine producing high mortality and economic loss due to the low productivity. In this review, the major virulence factors, antimicrobial susceptibility and genetic diversity of <em>C. perfringens</em> from chickens with necrotic enteritis are showed.
Mostrar más [+] Menos [-]