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Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae
2022
Sánchez Antonio | Contreras Antonio | Sánchez-Corral María L. | Martínez-Nista Carmen | Collado Soledad | Sáez José L. | Minguez Olga | Fe Christian de la
Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M. agalactiae. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test.
Mostrar más [+] Menos [-]Influence of natural and climatic conditions on the distribution and forms of contagious agalactia in sheep in Bessarabia, Ukraine
2022
Volodymyrovych Bohach Mykola | Igorovych Bolotin Vitaliy | Mykolaivych Bohach Denys | Piven Olha Tarasivna | Pyvovarova Iryna Victorivna
Contagious agalactia of ruminants is an endemic disease caused by Mycoplasma agalactiae in flicting significant losses on farms in deaths and forced slaughter of sick animals, abortions, births of sick young animals, and reduced milk and wool production. The aim of the study was to determine the influence of hydrometeorological conditions on the distribution and forms of contagious agalactia in sheep in Bessarabia, Ukraine.
Mostrar más [+] Menos [-]Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae
2022
Sánchez, Antonio | Contreras Martín, Antonio | Sánchez-Corral, María L. | Martínez-Nista, Carmen | Collado, Soledad | Sáez, José L. | Minguez, Olga | Fe, Christian de la
Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M. agalactiae. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test. This study was designed to appraise the performance of two commercial indirect ELISA tests using M. agalactiae p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different M. agalactiae strains. We carried out the test evaluation using sera of confirmed M. agalactiae-positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests. All three ELISA tests showed high validity scores (Youden’s J: 72.9–84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762–0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93). The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting M. agalactiae in milk and presenting clinical signs. The agreements show that any of these ELISA tests could be equally well used for diagnosis in programmes against CA.
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