A survey of 95 wild rats which were captured from various locations was conducted to determine the diversity and distribution of ectoparasites and endoparasites infesting wild rat population around the city of Ipoh and Kuala Lumpur. The rodents captured were Rat tus norvegicus and post mortem was carried out immediately after capture, with skin and organs examined for parasite infection. Ectoparasites recovered were blood sucking louse (Polyplax spinulosa) and mites (Myocoptes musculinus). Endoparasites recovered were nematodes (Aspiculuris tetraptera, Trichuris sp., and two strongyles, one of which is Strongyloides sp.) And three intestinal protozoan parasites (Blastocystis sp., Trichomonas sp., and a coccidia). Low diversity of ecto- and endoparasites were observed infecting wild rat population caught in Ipoh as compared to Kuala Lumpur.

Data over a period of eleven years was analysed for Infectious Bursal Disease (IBD) virus isolated from chicken samples submit ted to the Regional Veterinary Laboratory at Bukit Tengah, Malaysia (RVLBT) for diagnosis. A total of 247 suspect IBD cases were tested by Virology Section, RVLBT between years of 2006 to 2016. IBD virus has been isolated by using Agar Gel Precipitation Test (AGPT), a bursal homogenate which has been used as an antigen against a known positive antiserum. About 27 cases (11%) from a total of 247 suspect cases in chickens were positive for the presence of IBD. The rate of IBD may be influenced by age of chickens with an increase in the possibility of IBD occurring in chicken older than 3 weeks. Apart from that, both broiler and local chickens are highly susceptible to this disease. Therefore, awareness on the existing IBD cases indicates the importance of strict management procedures, proper management programmes, vaccination and immunisation for chickens in Malaysia.

A study was conducted to identify the current status of Fasciolaand Paramphistomum infections in small ruminants in Terengganu. A total of 267 faecal samples from small ruminants were collected and subjected to sedimentation technique. Serum samples were diagnosed for detection of IgG antibody for Fasciola infection using sELISA method. Results showed that there were 4% of the goats positive with Paramphistomum eggs whereas Fasciola egg was not observed in any of the faecal samples. However, it was found that 89% of the serum samples from goats were positive with IgG antibody for Fasciola infection. Small ruminants in Terengganu were not infected with severe Fasciola and Paramphistomum infections yet the results obtained from this study will update the current status of the infections. This information will help the farmers and the Department of Veterinary Services to plan on management to maintain the animals’ health.

Most studies on Simulum damnosum in Nigeria has focused mainly on the problem of onchocerciasis from the ecological standpoint. However, this study is aimed at viewing it from the human angle, with focus placed on the ignorance of the rural dwellers about the influences of their environment on the increasing incidences of onchocerciasis in their population. The premise is that, as the government and the scientists are working towards finding a lasting solution to the epidemic, the rural dwellers need to wake up to the realities of their own destiny. They need to come out and collaborate with other good efforts to form a strong united force that will rid their society of this menace. A study on the level of ignorance was carried out. It is assumed that their ignorance has kept the villagers relatively inactive and passive about freeing themselves. A descriptive method was used for this research and about 450 villagers were randomly selected and verbally interviewed. A questionnaire was designed and used as the instrument of the research. The research was carried out in two different villages of Asejire and Asepari. Findings were encouraging. Some of the villagers did not know about the existence of the disease even when some of their close relatives showed the symptoms. This high level of ignorance shows that it will be difficult to attain any cooperation from them in any attempt to make their environment unfavourable for the vector.

A total of 5,142 kidney tissue samples and 5,111 serum samples from mature cattle in 49 states and Puerto Rico were collected at slaughter. Age of cattle ranged from 1 to 16 years (mean, 6.6 years). Leptospires were isolated from 88 (1.7%) kidney tissues, and 2,493 (49%) sera contained antibodies against I or more of 12 Leptospira interrogans serovars. Leptospires were observed by immunofluorescence in 41 (0.8%) kidney tissues. Using agglutinin-absorption tests, 73 (83%) isolates were identified as serovar hardjo, 11 (12.5%) as serovar pomona, and 4 (4.5%) as serovar grippotyphosa. By use of restriction endonuclease analysis studies of chromosomal DNA, all isolates differed from reference serovars but were identical to strains previously isolated from cattle or swine in the United States. Of the serovar hardjo isolates, 85% were identical to restriction endonuclease analysis type (genotype) hardjo-bovis A and 11 (15%) were identical to genotype hardjo-bovis B. Serovar pomona isolates were identical to genotypes kennewicki A (64%) or kennewicki B (36%), and serovar grippotyphosa isolates were identical to the RM 52 strain. Isolation rates were significantly (P < 0.001) higher for beef cattle than for dairy cattle and were higher (P < 0.001) for bulls than for cows. Combined culture and immunofluorescence results indicated that 2% of mature cattle were renal carriers of leptospires.

Increased emergence in microbial resistance to antibiotics is a growing problem globally. A retrospective investigation was made of antimicrobial resistance in a total of 2,345 Escherichia coli isolates from clinical cases submitted to Regional Veterinary Laboratory of Bukit Tengah, Penang, Malaysia between January 2000 and December 2015. Analysis using WHONET 5.6 revealed that resistance to ampicillin, norfloxacin and aminoglycosides was detected in isolates from poultry and pigs more often than ruminants. Resistant to colistin was higher in ruminants compared to other animal groups. Nor floxacin susceptibility was very high in isolates from ruminants, pet birds (82.2%) and zoo animals (88.6%). Isolates from pet birds had higher resistant rate to all of the antimicrobials tested compared to zoo and companion animals. Multidrug-resistant (MDR) E. coli isolates (29.25%, 684/2345) were detected in this study. Further efforts, for instance, national monitoring and surveillance, are required to quantify the exposure of animals to antimicrobial agents and resistant pathogenic or commensal bacteria in the animals and also its environment. Such useful information indeed will assist the prudent use of antimicrobial agents in veterinary practice; therefore, suppress the emergence of antimicrobial resistance in animals.

Bovine haemoparasites are cosmopolitan in distribution and are known to cause substantial losses to the cattle industry. In spite of their economic importance, there remains a dearth of information on their molecular epidemiology in many parts of the world including Malaysia. To ascertain the molecular prevalence and species co-infection of bovine haemoparasites in the country, blood samples were collected from 1,045 heads of beef and dairy cattle on 43 farms from six geographical zones throughout Peninsular Malaysia. Samples subjected to PCR amplification of parasite species-specific genetic fragments revealed that Anaplasma marginale was the most prevalent haemoparasite (72.6%),followed by Theileria orientalis(49.8%),Candidatus Mycoplasma haemobos ( 47. 0 % ),Babesia bovis(32. 5%), Babesia bigemina (30.5%) and Trypanosomaevansi(17.9%). A high percentage (92.1%) of cattle was infected with either one or more haemoparasites. Triple haemoparasite species co-infection was the most prevalent (25.6%), followed closely by double species co-infection (25.1%). The most common (8.8%) and significantly correlated(rs= 0.250; p<0.01) combination was A. marginale+ T.orientalis. The present study constitutes the first attempt in the country to document the molecular prevalence and species co-infection of bovine haemoparasites over a wide spatial distribution. The data obtained will facilitate treatment, control and prevention measures to improve the local cattle industry.

A total of 65 Pasteurella multocida were isolated and identified from various animal’s samples received by Veterinary Research Institute (VRI) during the period of 2014 to 2016. These animals comprises of cattle, goat, pig, chicken, duck and rabbit. The serogroup of Pasteurella multocida were carried out using designation system of Carter’s capsular typing and molecular serogrouping method. Based on cases submitted to VRI, the prevalence of pasteurellosis in Malaysia ranging from 1.0% to 3.2% (2014 to 2016). It is low compared to previous reports and the pattern of predominant serogroups and animal hosts were found to be changing every year. In 2014, 80% (12/15) of the isolates were Pasteurella multocida Carter’s type D where all were isolated from goats. In 2015, the predominant serogroup changed to Pasteurella multocida Carter’s type A with a prevalence rate of 40.6% (13/32) which were mostly isolated from duck and cattle. While for Pasteurella multocida Carter’s type D, the prevalence in 2015 reduced to 21.9% (7/32) compared to the previous year and it was isolated from various animal species. Interestingly, in 2015 there was one isolate of Pasteurella multocida Carter’s type B isolated from goat with no reported history of outbreak. In 2016, the prevalence of Pasteurella multocida Carter’s type A increased to 72.2% (13/18), with a high percentage (92.3%) infection in young calves showing clinical signs with high mortality and morbidity in infected farms. Furthermore, during these 3 years of study, 3 isolates of Pasteurella multocida serogroup F were also identified each from pig, goat and chicken, respectively. In conclusion, this study revealed that pasteurellosis had become sporadic in Malaysia and the distribution of serogroups were diverse in all species of animal with no definitive host.

A Survey of gastrointestinal helminthes/ parasites in camel migrated from Tehsil Jalapur Pir Wala to Multan Tehsil, was carried out during May, 2012.A total number of 50 samples (20 males and 30 females) were collected from various places at Multan. The revealed parasites were mixed helminthic infection and identified as strongylidae spp, trichostrongyle spp, coccidian/eimeria spp and isospora spp.

Serum samples obtained from 40,927 swine at various locations in North Carolina between Aug 1, 1987 and July 31, 1988, were tested for antibodies to Trichinella spiralis, using an ELISA based on a larval T spiralis excretory-secretory antigen. In the ELISA, samples were considered to have positive results if the optical density (OD) reading was equal to or 5 times greater than the mean OD value of 4 negative-control sera from trichina-free swine. Of the 40,927 serum samples tested, 154 (0.38%) were positive by ELISA; the rate for breeding swine was 0.35% (105/30,162), and the rate for cull swine was 0.45% (49/10,765). Of the 49 seropositive samples from cull swine, 11 were from out of the state, 22 had no identification, and 16 were known to originate from North Carolina. Seropositivity had a bimodally seasonal distribution, with peaks in March and September. There was no difference between the mean age of seropositive and seronegative swine, but males were at greater risk for seropositivity than were females. Pigs from lots with < 100 sera tested were at increased risk for seropositivity, as were pigs from the central coastal region of North Carolina.