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Efficiency of silver nanoparticles against bacterial contaminants isolated from surface and ground water in Egypt
2015
Reem Dosoky | Saber Kotb | Mohamed Farghali
The bactericidal efficiency of silver nanoparticles (AgNP) was evaluated against bacteria isolated from surface and ground water samples in Egypt. The AgNP were synthesized by typical one-step synthesis protocol, and were characterized using transmission electron microscopy and atomic absorption spectrophotometer. The bactericidal efficiency of AgNP was evaluated by its application in three concentrations i.e., 0.1, 0.05 and 0.01 ppm to water sample, and allowed to interact with bacteria for different duration e.g., 5 min 15 min, 30 min, 1 h and 2 h. Then, the bactericidal efficiency of AgNPs was determined by comparing the counted bacteria before and after the treatments. Higher mean values of total bacterial count (TBC), total coliform count (TCC), and total streptococcal count (TFS) were detected in surface water than in ground water. Also, the results showed that TBC, TCC and TFS exceeded permissible limits. Application of AgNP at different concentration, the number of bacteria in TBC was significantly reduced in all AgNP-exposed samples as compared to the control group (p<0.05). The highest concentration of AgNP exhibited highest bactericidal efficiency in TBC, where, after two hours, 0.1, 0.05 and 0.01 mg/L AgNP was found to be sufficient to inhibit 91.85, 89.14 and 74.92%, and 92.33, 85.23 and 53.17% in TBC of surface and ground water, respectively. Moreover, the inhibition efficiency of the highest concentration (0.1 ppm) against TCC reached to 98.10 and 99.88% in surface water and 95.54 and 99.20% in ground water after 1 h and 2 h, respectively. Similar results were found against TFS count. The AgNPs were found to be effective against bacteria of water origin.
Mostrar más [+] Menos [-]Comparison between microscopic examination and competitive ELISA for diagnosis of equine piroplasmosis in Kelantan, Malaysia
2016
Azlinda A. B. | Arshad M. M. | Mohd Azam K. G. K. | Al-Obaidi, Q. T. | Al-Sultan I. I.
The objectives of the present study were to determine the infection rate of equine piroplasmosis (EP) in horses and ponies in Kelantan,Malaysia and compare the microscopic examination with competitive enzymelinked immunosorbent assay (cELISA) test as methods for diagnosis of EP. 306 blood samples were randomly collected from equids including 148 horses and 158 ponies in various districts of Kelantan, from September 2013 to March 2014. Based on microscopic examination of the staining blood smears, the infection rates ofTheileria equi, Babesia caballi and of both infections in horses were 19.59%, 25% and 8.78% respectively, whereas in ponies theinfection rates were 14.55%, 19.62%, and 5.69% respectively. Based on cELISA test, the infection rates of T. equi, B. caballi and of both infections in horses were 50.67%, 62.16% and 33.10% respectively,whereas in ponies, the infection rates were 51.89%, 63.92% and 35.44% respectively. No significant difference were observed between equids species associated with a seroprevalence of T. equi, B. caballi andof both infections (P≤ 0.05). According to the Kappa value there was no compatibility between microscopic examination and cELISA on the diagnosis of T. equi, B. caballi and of both infections which were 0.235, 0.013 and 0.080 respectively. In conclusion, the current results for this research work indicate that equine piroplasmosis is widespread in Kelantan, Malaysia and cELISA test is more efficientthan microscopic examination for diagnosis of EP.
Mostrar más [+] Menos [-]Use of a multiple logistic regression model to determine prognosis of dairy cows with right displacement of the abomasum or abomasal volvulus
1990
Grohn, Y.T. | Fubini, S.L. | Smith, D.F.
Data at admission and at surgery were collected on 458 cows with right displacement of the abomasum or abomasal volvulus, to derive multiple logistic regression models for predicting postsurgical outcome (productive, salvaged, or terminal). The derived models contained few and easily obtained variables. The weight associated with each variable was determined objectively. Three admission variables (heart rate, base excess, and plasma chloride concentration), and 5 surgical variables (heart rate, base excess, diagnosis, method of decompression used, and appearance of abomasal serosa) were used in the final models. Predicted outcomes that used the admission and surgical models were closely related with actual outcomes. Total correct classification for satisfactory (productive) versus unsatisfactory outcome (salvaged and terminal) was 78.2% for the admission model and 82.7% for the surgical model. Combining data on cows with productive and salvaged outcomes as satisfactory outcome, and terminal as unsatisfactory outcome, total correct classification was 90.7% for the admission model and 93.2% for the surgical model. Using predicted probabilities, the market value of productive and salvaged cows, and the medical and surgical costs, one can calculate the expected economic value of each outcome. Treatment can be justified if the sum of the expected value of productive and salvaged outcome exceeds the sum of the medical and surgical costs and the expected salvaged value of the cow that was not treated surgically.
Mostrar más [+] Menos [-]Influence of calving age, parity, and calf gender on lactation metrics, reproductive and economic efficiency parameters in Egyptian buffaloes
2024
Hadeel S. El-Qaliouby | Nagwa I. El-Kasrawy | Hassaballah A. Abouelghait | Mohamed M. Hegazy | Ayman H. Abd El-Aziz
Buffalo reproductive performance and sustained productivity measures remain inadequately studied. This study aimed to investigate the influences of calving age, parity, and calf gender on specific indicators, including days in milk (DIM), daily milk yield (DMY), total milk yield (TMY), and 270-days milk yield (270-DMY), alongside reproductive and economic parameters. Analyzing 2112 buffalo cow datasets from 1994-2022, acquired from five distinct farms associated with Egypt's Animal Health Research Institute (AHRI) in Kafr El-Sheikh province, notable findings emerged. There was a marked influence of calving age and parity order on the milk yields, with buffaloes aged 8-10 years exhibiting peak yields. A significant uptrend was observed in milk yield metrics from the initial to the seventh parity. Notably, reproductive durations, including days open, calving intervals, and dry periods, peaked during the first parity. Economic evaluations revealed that first parity buffaloes recorded the least production expenditures. In contrast, seventh lactation buffaloes were the most profitable. A noteworthy observation was that buffalo calving less than six years demonstrated reduced production costs. Net profit showed the peak within the age of less than 6 to 8 years before tapering off. Contrarily, calf gender remained inconsequential across the studied parameters. The study highlights the importance of integrating production, reproductive, and economic determinants in buffalo breeding optimization strategies.
Mostrar más [+] Menos [-]Antibacterial and therapeutic effects of a combination of Sophora flavescens and Glycyrrhiza uralensis Fischer ethanol extracts on mice infected with Streptococcus pyogenes
2014
Yu, E.A., Tongyeong National Quarantine Station, Ministry of Health & Welfare, Tongyeong, Republic of Korea | Cha, C.N., Gyeongsang National University, Jinju, Republic of Korea | Park, E.K., (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University, Busan, Republic of Korea | Yoo, C.Y., Department of Computer Information, Gyeongnam Provincial Namhae College, Namhae, Republic of Korea | Kim, S., Gyeongsang National University, Jinju, Republic of Korea | Lee, H.J., Gyeongsang National University, Jinju, Republic of Korea
This study evaluated the antibacterial effects of a mixture of Sophorae radix and Glycyrrhiza uralensis Fischer (1 : 1) ethanol extracts (SGE) on mice infected with Streptococcus (S.) pyogenes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of SGE necessary for antibacterial effects against S. pyogenes were 20§¡/mL. Based on the time-kill curves for S. pyogenes, SGE was effective at 4 ¡¿MIC after 16 h. On Day 12 after challenge, the survival rate of mice treated with 2.0 mg/kg SGE was 60%. In conclusion, SGE had potent in vitro and in vivo antibacterial activities against S. pyogenes.
Mostrar más [+] Menos [-]Resistance to trypanocidal drugs - suggestions from field survey on drug use in Kwale district, Kenya
2003
Mugunieri, G.L. | Murilla, G.A. (Kenya Trypanosomiasis Research Inst., Kikuyu (Kenya))