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Adhesion of bacteria to the cecal mucosal surface of conventional and germ-free chickens infected with Eimeria tenella.
1992
Baba E. | Wakeshima H. | Fukui K. | Fukata T. | Arakawa A.
When Salmonella typhimurium and Clostridium perfringens were tested in conventional chickens, larger numbers of S typhimurium and C perfringens adhered to Eimeria tenella-infected ceca than to uninfected ceca. In germ-free chickens, S typhimurium and C perfringens adhered to the E tenella-infected cecal mucosa more than to the uninfected cecal mucosa, but fewer Bacteroides vulgatus and Bifidobacterium thermophilum adhered to the E tenella-infected ceca than to the uninfected ceca. Many bacteria adhered to the lesions caused by E tenella as observed by scanning electron microscopy. On the basis of our findings, we suggest that infection with E tenella upsets the balance of competitive adherence of bacteria, allowing more colonization of S typhimurium and C perfringens.
Mostrar más [+] Menos [-]Increase of mannose residues, as Salmonella typhimurium-adhering factor, on the cecal mucosa of germ-free chickens infected with Eimeria tenella
1993
Baba, W. | Tsukamoto, Y. | Fukata, T. | Sasai, K. | Arakawa, A.
To study increase of the Salmonella population in the cecum of chickens infected with Eimeria tenella, quantitative changes in mannose residues on the cecal mucosa were investigated. Inhibition of S typhimurium adherence to the cecum by a 2% carbohydrate (D-mannose, D-galactose, L-fucose, alpha-methyl-D-glucoside) in phosphate-buffered saline solution was examined. Only D-mannose had inhibitory effects. Whereas, D-galactose had somewhat enhancing effects on adherence of S typhimurium to the cecal mucosa of uninfected germ-free chickens. In infected and uninfected chickens, D-mannose inhibited adherence of S typhimurium. D-mannose significantly (P < 0.05) increased adherence of Bacteroides sp. In infected and uninfected chickens, D-mannose did not have any effect on adherence of Clostridium perfringens and Bifidobacterium thermophilum. Under microscopic observation, only concanavalin A and Lens culinaris agglutinin, of 8 lectins examined, were recognized as lectin-positive staining lines or spots in the cecal mucosa, indicating presence of mannose residues on the cecal mucosa. In E tenella-infected chickens, lectin-positive staining was seen strongly on the coarse surface of damaged cells and at the bottom of the crypts. These results indicate that coccidial infection may induce increase of mannose residues on the intestinal surface and allow adhesion of more salmonellae to the intestine.
Mostrar más [+] Menos [-]Alpha-difluoromethylornithine: A promising lead for preventive chemotherapy for coccidiosis
1982
Hanson, W.L. | Bradford, M.M. | Chapman, W.L., jr | Waits, V.B. | McCann, P.P.
Eimeria tenella, chickens (exper.), alpha-difluoromethylornithine promising for preventive chemotherapy, perhaps prevents schizont formation, anticoccidial effect possibly due to inhibition of putrescine biosynthesis; immunity to reinfection after treatment
Mostrar más [+] Menos [-]Anti-parasitic activity of zinc oxide nanoparticles against Eimeria tenella in broilers experimentally infected
2022
Anah, S.A. | Anah, S.A. | Al-Khalidy, K.A.H.
In the study, zinc oxide nanoparticles (ZNOPs) at concentrations of 20, 40, and 60 mg/kg were tested for their antimicrobial action against the oocysts of Eimeria tenella. The oocysts of E. tenella were isolated from the feces of broilers received at the veterinary hospital in Diwaniyah Province and initially diagnosed by compound optical microscopy. The oocysts were confirmed molecularly by polymerase chain reaction targeting the ITS1 gene with a molecular weight of 409 bp. The results in the first week showed that ZNOP concentrations of 20 and 40 mg/kg possess various activities against E. tenella, while 60 mg/kg was the most effective in reducing excreted oocysts compared to the positive control and amprolium group, along with the appearance of mild symptoms and a mortality rate of 0.8%. In the second week of infection, excreted oocysts and mortality rates generally decreased in all treated groups. A comparison of all groups showed that the 60 mg/kg ZNOP-treated group had a significantly lower number of excreted oocysts, and all birds in this group recovered during the second week of infection. These findings revealed the prospect of using ZNOPs against E. tenella in challenging situations of the appearance of resistance to anticoccidial agents.
Mostrar más [+] Menos [-]Adhesion of bacteria to the cecal mucosal surface of conventional and germ-free chickens infected with Eimeria tenella
1992
Baba, E. | Wakeshima, H. | Fukui, K. | Fukata, T. | Arakawa, A.
When Salmonella typhimurium and Clostridium perfringens were tested in conventional chickens, larger numbers of S typhimurium and C perfringens adhered to Eimeria tenella-infected ceca than to uninfected ceca. In germ-free chickens, S typhimurium and C perfringens adhered to the E tenella-infected cecal mucosa more than to the uninfected cecal mucosa, but fewer Bacteroides vulgatus and Bifidobacterium thermophilum adhered to the E tenella-infected ceca than to the uninfected ceca. Many bacteria adhered to the lesions caused by E tenella as observed by scanning electron microscopy. On the basis of our findings, we suggest that infection with E tenella upsets the balance of competitive adherence of bacteria, allowing more colonization of S typhimurium and C perfringens.
Mostrar más [+] Menos [-]Use of monoclonal antibodies directed against Eimeria tenella sporozoites to determine stage specificity and in vitro effect on parasite penetration and development
1983
Danforth, H.D.
3 hybridoma-produced monoclonal antibody cell lines elicited against Eimeria tenella sporozoites showed various degrees of reactivity with the other intracellular developmental stages, all inhibited sporozoite-host cell penetration and coccidial development in vitro
Mostrar más [+] Menos [-]Immunohistologic examination of monoclonal antibodies generated against Eimeria bovis sporozoites for reactivity to meronts and sexual stages of E bovis and other eimerian parasites
1991
Lindsay, D.S. | Dubey, J.P. | Fayer, R.
Seven monoclonal antibodies (MAB) generated against sporozoites of Eimeria bovis were tested for reactivity against immature and mature first-generation meronts, sexual stages, and oocysts in tissues from experimentally infected calves by use of an avidin-biotin peroxidase complex (ABPC) immunohistologic test. Three of the 7 MAB reacted in the ABPC test. One of these, MAB-4FB4, reacted only with mature E bovis meronts. The other 2 MAB, MAB2AE7 and MAB4AD7, reacted with all the developmental stages of E bovis tested. Asexual stages and sexual stages of E tenella from chickens and E papillata from mice also were examined in the ABPC test. Monoclonal antibodies MAB-2AE7 and MAB-4AD7 reacted with all stages of these eimerian protozoa. None of the other 5 MAB reacted with these parasites. Results of this study suggested that antigens are shared among the asexual and sexual stages of several diverse Eimeria species.
Mostrar más [+] Menos [-]Oocysticidal effects of morestan combination preparations against oocysts of Eimeria tenella
1994
Noh, J.W. | Kim, B.G. (Bayer Vetchem Ltd., Anyang (Korea Republic). Bayer Veterinary Medical Research Institute) | Oh, H.G. (Seoul National University, Seoul (Korea Republic). College of Veterinary Medicine)
Effects of gamma-irradiation from cobalt-60 on pathogenicity of Eimeria tenella
1993
Youn, H.J. | Kang, Y.B. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute) | Jang, D.H. (Seoul National University, Seoul (Korea Republic). College of Veterinary Medicine)
Immunization of young chicks using graded dose of wild strain of Eimeria tenella
Kimbita, E.N.(Sokoine Agricultural Univ., Morogoro (Tanzania). Dept. of Veterinary and Microbiology and Parasitology) | Samani, P.M. | Machange, E.N. | Silayo, R.S.