Results of chemical and toxicological estimation of an energized preparation, intended for increasing of performance ability and sports qualities of horses realized in the conditions of the Republic of Belarus were presented. The energized preparation included honey, lactalbumin hydrolyzate, pollen hydrolyzate, and teotropin. Chemical and toxicological estimation of the preparation included determination of physical, chemical and pharmacological compatibility of components of the preparation, acute and chronic toxicological characteristics, skin-resorbtive poison after single and long-term application at horse skin, topical-product-induced irritation and allergic action of the preparation. Research results showed that all components of the preparation were compatible among themselves. The preparation proved to be a low-hazardous substance (substance hazard category 4) and did not possess skin-absorptive, local-irritating and allergic action.