Four intrauterine treatment strategies were evaluated for effectiveness in mares that were confirmed to be susceptible to chronic uterine infection. Pretreatment samples were obtained at detection of estrus, and a genital strain of Streptococcus zooepidemicus was infused into the uterus when a preovulatory (> 35 mm) follicle was detected. At 12 hours after inoculation, mares were assigned to 1 of 4 selected treatment groups: autologous plasma, 100 ml (n = 5); potassium penicillin, 5 million U in 100 ml of phosphate-buffered saline solution (PBSS; n = 5); 10 mg of prostaglandin F2alpha in 100 ml of PBSS (n = 5); and large-volume lavage with normal saline solution (1,000 ml increments). A fifth group, treated with vehicle alone (100 ml of PBSS), served as a negative control (n = 7). All treatments were administered into the uterus. To assess the effectiveness of the treatment, samples for culture and cytologic examination were collected at 96 hours after bacterial inoculation. An effect of treatment was observed on the number of uterine neutrophils (P = 0.02) and growth of S zooepidemicus (P < 0.01). Intrauterine treatment with potassium penicillin, prostaglandin F2alpha, and large-volume uterine lavage significantly reduced the growth of S zooepidemicus (P < 0.01) as well as the number of neutrophils (P < 0.02). Autologous plasma reduced the number of neutrophils (P < 0.05), but not growth of S zooepidemicus. There was significant correlation between the number of uterine neutrophils and growth of S zooepidemicus for each treatment group (r = 0.57; P < 0.05).

The increasing number of diseases with urogenital chlamydiosis became a serious problem for the modern veterinary science. The importance of urogenital clamidiosis in the infectious pathology of animals was presented by the direct multifocal affect of urogenital system and after-effects of a disease influencing the reproductive function, as well as the potential hazard of its transformation into a source of chlamydia infections of other localization. In the conditions of the Republic of Belarus the chlamydial endometritis of cattle in the majority of cases was associated with Staphylococcus aureus in 25,7% of cows. Chlamydial endometritis of cows was usually preceded by: embryotocia (9,9%), pathological calving (4,4%), retention of placenta (14,8%), subinvolution of uterus (27,1%). The biochemical tests in trial group showed the 23% decrease of carotin on twofold decrease vitamin A. In course of the immunologic studies it was established, that dilution of A class immunoglobulins was on 31% higher in animals of a trial group in comparison with the control group. The level of IgG immunoglobulin G and immunoglobulin M slightly differed between groups